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PHOU_PSEAE
ID   PHOU_PSEAE              Reviewed;         242 AA.
AC   Q51547;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 122.
DE   RecName: Full=Phosphate-specific transport system accessory protein PhoU homolog;
DE            Short=Pst system accessory protein PhoU homolog;
GN   Name=phoU; OrderedLocusNames=PA5365;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=8628229; DOI=10.1007/bf02172980;
RA   Nikata T., Sakai Y., Shibata K., Kato J., Kuroda A., Ohtake H.;
RT   "Molecular analysis of the phosphate-specific transport (pst) operon of
RT   Pseudomonas aeruginosa.";
RL   Mol. Gen. Genet. 250:692-698(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=8083184; DOI=10.1128/jb.176.18.5874-5877.1994;
RA   Kato J., Sakai Y., Nikata T., Ohtake H.;
RT   "Cloning and characterization of a Pseudomonas aeruginosa gene involved in
RT   the negative regulation of phosphate taxis.";
RL   J. Bacteriol. 176:5874-5877(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Plays a role in the regulation of phosphate uptake.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: Mutants are constitutive of alkaline phosphatase
CC       and show chemotactic response to phosphate regardless of wheather the
CC       cells are starved for phosphate. {ECO:0000269|PubMed:8083184}.
CC   -!- SIMILARITY: Belongs to the PhoU family. {ECO:0000305}.
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DR   EMBL; D45195; BAA08138.1; -; Genomic_DNA.
DR   EMBL; D28587; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AE004091; AAG08750.1; -; Genomic_DNA.
DR   PIR; S68596; S65576.
DR   RefSeq; NP_254052.1; NC_002516.2.
DR   RefSeq; WP_003096661.1; NZ_QZGE01000020.1.
DR   PDB; 4Q25; X-ray; 2.28 A; A/B=1-242.
DR   PDBsum; 4Q25; -.
DR   AlphaFoldDB; Q51547; -.
DR   SMR; Q51547; -.
DR   STRING; 287.DR97_2736; -.
DR   PaxDb; Q51547; -.
DR   PRIDE; Q51547; -.
DR   EnsemblBacteria; AAG08750; AAG08750; PA5365.
DR   GeneID; 881627; -.
DR   KEGG; pae:PA5365; -.
DR   PATRIC; fig|208964.12.peg.5622; -.
DR   PseudoCAP; PA5365; -.
DR   HOGENOM; CLU_078518_2_1_6; -.
DR   InParanoid; Q51547; -.
DR   OMA; WKHGIET; -.
DR   PhylomeDB; Q51547; -.
DR   BioCyc; PAER208964:G1FZ6-5487-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0060326; P:cell chemotaxis; IMP:UniProtKB.
DR   GO; GO:0030643; P:cellular phosphate ion homeostasis; IEA:InterPro.
DR   GO; GO:0050922; P:negative regulation of chemotaxis; IMP:PseudoCAP.
DR   GO; GO:0045936; P:negative regulation of phosphate metabolic process; ISS:UniProtKB.
DR   GO; GO:2000186; P:negative regulation of phosphate transmembrane transport; ISS:UniProtKB.
DR   GO; GO:0050928; P:negative regulation of positive chemotaxis; IMP:PseudoCAP.
DR   GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.58.220; -; 2.
DR   InterPro; IPR028366; P_transport_PhoU.
DR   InterPro; IPR038078; PhoU-like_sf.
DR   InterPro; IPR026022; PhoU_dom.
DR   PANTHER; PTHR42930; PTHR42930; 1.
DR   Pfam; PF01895; PhoU; 2.
DR   PIRSF; PIRSF003107; PhoU; 1.
DR   TIGRFAMs; TIGR02135; phoU_full; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chemotaxis; Cytoplasm; Phosphate transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..242
FT                   /note="Phosphate-specific transport system accessory
FT                   protein PhoU homolog"
FT                   /id="PRO_0000155175"
FT   HELIX           15..45
FT                   /evidence="ECO:0007829|PDB:4Q25"
FT   HELIX           49..56
FT                   /evidence="ECO:0007829|PDB:4Q25"
FT   HELIX           59..78
FT                   /evidence="ECO:0007829|PDB:4Q25"
FT   HELIX           84..120
FT                   /evidence="ECO:0007829|PDB:4Q25"
FT   HELIX           127..149
FT                   /evidence="ECO:0007829|PDB:4Q25"
FT   HELIX           153..183
FT                   /evidence="ECO:0007829|PDB:4Q25"
FT   HELIX           185..187
FT                   /evidence="ECO:0007829|PDB:4Q25"
FT   HELIX           188..220
FT                   /evidence="ECO:0007829|PDB:4Q25"
SQ   SEQUENCE   242 AA;  27487 MW;  BBA58E297E6C973C CRC64;
     MINKDSLTHH ISQQFNAELE DVRSHLLAMG GLVEKQVNDA VNALIDADSG LAQQVREIDD
     QINQMERNID EECVRILARR QPAASDLRLI ISISKSVIDL ERIGDEASKV ARRAIQLCEE
     GESPRGYVEV RHIGSQVQKM VQEALDAFAR FDADLALSVA QYDKTVDREY KTALRELVTY
     MMEDPRAISR VLNIIWALRS LERIGDHARN IAELVIYLVR GTDVRHIGLT RMKEEVENNR
     GE
 
 
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