PHOU_PSEAE
ID PHOU_PSEAE Reviewed; 242 AA.
AC Q51547;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Phosphate-specific transport system accessory protein PhoU homolog;
DE Short=Pst system accessory protein PhoU homolog;
GN Name=phoU; OrderedLocusNames=PA5365;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8628229; DOI=10.1007/bf02172980;
RA Nikata T., Sakai Y., Shibata K., Kato J., Kuroda A., Ohtake H.;
RT "Molecular analysis of the phosphate-specific transport (pst) operon of
RT Pseudomonas aeruginosa.";
RL Mol. Gen. Genet. 250:692-698(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8083184; DOI=10.1128/jb.176.18.5874-5877.1994;
RA Kato J., Sakai Y., Nikata T., Ohtake H.;
RT "Cloning and characterization of a Pseudomonas aeruginosa gene involved in
RT the negative regulation of phosphate taxis.";
RL J. Bacteriol. 176:5874-5877(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Plays a role in the regulation of phosphate uptake.
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Mutants are constitutive of alkaline phosphatase
CC and show chemotactic response to phosphate regardless of wheather the
CC cells are starved for phosphate. {ECO:0000269|PubMed:8083184}.
CC -!- SIMILARITY: Belongs to the PhoU family. {ECO:0000305}.
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DR EMBL; D45195; BAA08138.1; -; Genomic_DNA.
DR EMBL; D28587; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AE004091; AAG08750.1; -; Genomic_DNA.
DR PIR; S68596; S65576.
DR RefSeq; NP_254052.1; NC_002516.2.
DR RefSeq; WP_003096661.1; NZ_QZGE01000020.1.
DR PDB; 4Q25; X-ray; 2.28 A; A/B=1-242.
DR PDBsum; 4Q25; -.
DR AlphaFoldDB; Q51547; -.
DR SMR; Q51547; -.
DR STRING; 287.DR97_2736; -.
DR PaxDb; Q51547; -.
DR PRIDE; Q51547; -.
DR EnsemblBacteria; AAG08750; AAG08750; PA5365.
DR GeneID; 881627; -.
DR KEGG; pae:PA5365; -.
DR PATRIC; fig|208964.12.peg.5622; -.
DR PseudoCAP; PA5365; -.
DR HOGENOM; CLU_078518_2_1_6; -.
DR InParanoid; Q51547; -.
DR OMA; WKHGIET; -.
DR PhylomeDB; Q51547; -.
DR BioCyc; PAER208964:G1FZ6-5487-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR GO; GO:0060326; P:cell chemotaxis; IMP:UniProtKB.
DR GO; GO:0030643; P:cellular phosphate ion homeostasis; IEA:InterPro.
DR GO; GO:0050922; P:negative regulation of chemotaxis; IMP:PseudoCAP.
DR GO; GO:0045936; P:negative regulation of phosphate metabolic process; ISS:UniProtKB.
DR GO; GO:2000186; P:negative regulation of phosphate transmembrane transport; ISS:UniProtKB.
DR GO; GO:0050928; P:negative regulation of positive chemotaxis; IMP:PseudoCAP.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.58.220; -; 2.
DR InterPro; IPR028366; P_transport_PhoU.
DR InterPro; IPR038078; PhoU-like_sf.
DR InterPro; IPR026022; PhoU_dom.
DR PANTHER; PTHR42930; PTHR42930; 1.
DR Pfam; PF01895; PhoU; 2.
DR PIRSF; PIRSF003107; PhoU; 1.
DR TIGRFAMs; TIGR02135; phoU_full; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chemotaxis; Cytoplasm; Phosphate transport;
KW Reference proteome; Transport.
FT CHAIN 1..242
FT /note="Phosphate-specific transport system accessory
FT protein PhoU homolog"
FT /id="PRO_0000155175"
FT HELIX 15..45
FT /evidence="ECO:0007829|PDB:4Q25"
FT HELIX 49..56
FT /evidence="ECO:0007829|PDB:4Q25"
FT HELIX 59..78
FT /evidence="ECO:0007829|PDB:4Q25"
FT HELIX 84..120
FT /evidence="ECO:0007829|PDB:4Q25"
FT HELIX 127..149
FT /evidence="ECO:0007829|PDB:4Q25"
FT HELIX 153..183
FT /evidence="ECO:0007829|PDB:4Q25"
FT HELIX 185..187
FT /evidence="ECO:0007829|PDB:4Q25"
FT HELIX 188..220
FT /evidence="ECO:0007829|PDB:4Q25"
SQ SEQUENCE 242 AA; 27487 MW; BBA58E297E6C973C CRC64;
MINKDSLTHH ISQQFNAELE DVRSHLLAMG GLVEKQVNDA VNALIDADSG LAQQVREIDD
QINQMERNID EECVRILARR QPAASDLRLI ISISKSVIDL ERIGDEASKV ARRAIQLCEE
GESPRGYVEV RHIGSQVQKM VQEALDAFAR FDADLALSVA QYDKTVDREY KTALRELVTY
MMEDPRAISR VLNIIWALRS LERIGDHARN IAELVIYLVR GTDVRHIGLT RMKEEVENNR
GE
