ID   PHOU_STRPN              Reviewed;         216 AA.
AC   P0A3Y7; Q9X4T4;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Phosphate-specific transport system accessory protein PhoU homolog;
DE            Short=Pst system accessory protein PhoU homolog;
GN   Name=phoU; OrderedLocusNames=SP_2088;
OS   Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Streptococcus.
OX   NCBI_TaxID=170187;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-334 / TIGR4;
RX   PubMed=11463916; DOI=10.1126/science.1061217;
RA   Tettelin H., Nelson K.E., Paulsen I.T., Eisen J.A., Read T.D.,
RA   Peterson S.N., Heidelberg J.F., DeBoy R.T., Haft D.H., Dodson R.J.,
RA   Durkin A.S., Gwinn M.L., Kolonay J.F., Nelson W.C., Peterson J.D.,
RA   Umayam L.A., White O., Salzberg S.L., Lewis M.R., Radune D.,
RA   Holtzapple E.K., Khouri H.M., Wolf A.M., Utterback T.R., Hansen C.L.,
RA   McDonald L.A., Feldblyum T.V., Angiuoli S.V., Dickinson T., Hickey E.K.,
RA   Holt I.E., Loftus B.J., Yang F., Smith H.O., Venter J.C., Dougherty B.A.,
RA   Morrison D.A., Hollingshead S.K., Fraser C.M.;
RT   "Complete genome sequence of a virulent isolate of Streptococcus
RT   pneumoniae.";
RL   Science 293:498-506(2001).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) IN COMPLEX WITH ZINC IONS.
RG   Midwest center for structural genomics (MCSG);
RT   "Crystal structure of the phosphate transport system regulatory protein
RT   phoU from Streptococcus pneumoniae.";
RL   Submitted (SEP-2006) to the PDB data bank.
CC   -!- FUNCTION: Plays a role in the regulation of phosphate uptake.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PhoU family. {ECO:0000305}.
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DR   EMBL; AE005672; AAK76148.1; -; Genomic_DNA.
DR   PIR; C95244; C95244.
DR   RefSeq; WP_001245781.1; NZ_AKVY01000001.1.
DR   PDB; 2I0M; X-ray; 2.40 A; A=1-216.
DR   PDBsum; 2I0M; -.
DR   AlphaFoldDB; P0A3Y7; -.
DR   SMR; P0A3Y7; -.
DR   STRING; 170187.SP_2088; -.
DR   EnsemblBacteria; AAK76148; AAK76148; SP_2088.
DR   GeneID; 60232703; -.
DR   GeneID; 66807168; -.
DR   KEGG; spn:SP_2088; -.
DR   eggNOG; COG0704; Bacteria.
DR   OMA; DAMYNSL; -.
DR   PhylomeDB; P0A3Y7; -.
DR   BioCyc; SPNE170187:G1FZB-2173-MON; -.
DR   EvolutionaryTrace; P0A3Y7; -.
DR   Proteomes; UP000000585; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0030643; P:cellular phosphate ion homeostasis; IEA:InterPro.
DR   GO; GO:0045936; P:negative regulation of phosphate metabolic process; ISS:UniProtKB.
DR   GO; GO:2000186; P:negative regulation of phosphate transmembrane transport; ISS:UniProtKB.
DR   GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.58.220; -; 1.
DR   InterPro; IPR028366; P_transport_PhoU.
DR   InterPro; IPR038078; PhoU-like_sf.
DR   InterPro; IPR026022; PhoU_dom.
DR   PANTHER; PTHR42930; PTHR42930; 1.
DR   Pfam; PF01895; PhoU; 2.
DR   PIRSF; PIRSF003107; PhoU; 1.
DR   TIGRFAMs; TIGR02135; phoU_full; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Phosphate transport; Transport.
FT   CHAIN           1..216
FT                   /note="Phosphate-specific transport system accessory
FT                   protein PhoU homolog"
FT                   /id="PRO_0000155179"
FT   HELIX           5..35
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   HELIX           39..66
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   HELIX           74..105
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   HELIX           117..136
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   HELIX           137..139
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   HELIX           142..150
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   HELIX           152..168
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   TURN            169..171
FT                   /evidence="ECO:0007829|PDB:2I0M"
FT   HELIX           177..209
FT                   /evidence="ECO:0007829|PDB:2I0M"
SQ   SEQUENCE   216 AA;  24192 MW;  58E2C3ABBE653B4B CRC64;
     MRNQFDLELH ELEQSFLGLG QLVLETASKA LLALASKDKE MAELIINKDH AINQGQSAIE
     LTCARLLALQ QPQVSDLRFV ISIMSSCSDL ERMGDHMAGI AKAVLQLKEN QLAPDEEQLH
     QMGKLSLSML ADLLVAFPLH QASKAISIAQ KDEQIDQYYY ALSKEIIGLM KDQETSIPNG
     TQYLYIIGHL ERFADYIANI CERLVYLETG ELVDLN