PHP14_MOUSE
ID PHP14_MOUSE Reviewed; 124 AA.
AC Q9DAK9;
DT 08-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=14 kDa phosphohistidine phosphatase;
DE EC=3.9.1.3 {ECO:0000250|UniProtKB:Q9NRX4};
DE AltName: Full=Phosphohistidine phosphatase 1;
DE Short=PHPT1;
DE AltName: Full=Protein histidine phosphatase;
DE Short=PHP;
GN Name=Phpt1; Synonyms=Php14;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Exhibits phosphohistidine phosphatase activity.
CC {ECO:0000250|UniProtKB:Q9NRX4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N(pros)-phospho-L-histidyl-[protein] = L-histidyl-
CC [protein] + phosphate; Xref=Rhea:RHEA:47964, Rhea:RHEA-COMP:9745,
CC Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15377, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:64837; EC=3.9.1.3;
CC Evidence={ECO:0000250|UniProtKB:Q9NRX4};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N(tele)-phospho-L-histidyl-[protein] = L-histidyl-
CC [protein] + phosphate; Xref=Rhea:RHEA:47960, Rhea:RHEA-COMP:9745,
CC Rhea:RHEA-COMP:10719, ChEBI:CHEBI:15377, ChEBI:CHEBI:29979,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:83586; EC=3.9.1.3;
CC Evidence={ECO:0000250|UniProtKB:Q9NRX4};
CC -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q9NRX4}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the janus family. {ECO:0000305}.
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DR EMBL; AK005756; BAB24222.1; -; mRNA.
DR EMBL; BC028657; AAH28657.1; -; mRNA.
DR CCDS; CCDS15781.1; -.
DR RefSeq; NP_083569.1; NM_029293.2.
DR AlphaFoldDB; Q9DAK9; -.
DR SMR; Q9DAK9; -.
DR IntAct; Q9DAK9; 1.
DR MINT; Q9DAK9; -.
DR STRING; 10090.ENSMUSP00000037417; -.
DR iPTMnet; Q9DAK9; -.
DR PhosphoSitePlus; Q9DAK9; -.
DR REPRODUCTION-2DPAGE; IPI00118757; -.
DR REPRODUCTION-2DPAGE; Q9DAK9; -.
DR CPTAC; non-CPTAC-3732; -.
DR EPD; Q9DAK9; -.
DR jPOST; Q9DAK9; -.
DR MaxQB; Q9DAK9; -.
DR PaxDb; Q9DAK9; -.
DR PeptideAtlas; Q9DAK9; -.
DR PRIDE; Q9DAK9; -.
DR ProteomicsDB; 289553; -.
DR Antibodypedia; 18807; 382 antibodies from 31 providers.
DR DNASU; 75454; -.
DR Ensembl; ENSMUST00000039156; ENSMUSP00000037417; ENSMUSG00000036504.
DR GeneID; 75454; -.
DR KEGG; mmu:75454; -.
DR UCSC; uc008isp.1; mouse.
DR CTD; 29085; -.
DR MGI; MGI:1922704; Phpt1.
DR VEuPathDB; HostDB:ENSMUSG00000036504; -.
DR eggNOG; ENOG502S4DR; Eukaryota.
DR GeneTree; ENSGT00390000002738; -.
DR HOGENOM; CLU_120717_0_0_1; -.
DR InParanoid; Q9DAK9; -.
DR OMA; MCTQTRA; -.
DR OrthoDB; 1529401at2759; -.
DR PhylomeDB; Q9DAK9; -.
DR TreeFam; TF315158; -.
DR BRENDA; 3.9.1.3; 3474.
DR BioGRID-ORCS; 75454; 4 hits in 76 CRISPR screens.
DR ChiTaRS; Phpt1; mouse.
DR PRO; PR:Q9DAK9; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9DAK9; protein.
DR Bgee; ENSMUSG00000036504; Expressed in lens of camera-type eye and 85 other tissues.
DR Genevisible; Q9DAK9; MM.
DR GO; GO:0005829; C:cytosol; IDA:BHF-UCL.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0019855; F:calcium channel inhibitor activity; ISO:MGI.
DR GO; GO:0101006; F:protein histidine phosphatase activity; ISO:MGI.
DR GO; GO:0044325; F:transmembrane transporter binding; ISO:MGI.
DR GO; GO:2000984; P:negative regulation of ATP citrate synthase activity; ISO:MGI.
DR GO; GO:0051350; P:negative regulation of lyase activity; ISO:MGI.
DR GO; GO:0050860; P:negative regulation of T cell receptor signaling pathway; ISO:MGI.
DR GO; GO:0035971; P:peptidyl-histidine dephosphorylation; ISO:MGI.
DR GO; GO:2000147; P:positive regulation of cell motility; IMP:BHF-UCL.
DR GO; GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; ISO:MGI.
DR GO; GO:0006470; P:protein dephosphorylation; ISO:MGI.
DR GO; GO:2000249; P:regulation of actin cytoskeleton reorganization; ISO:MGI.
DR Gene3D; 3.50.20.20; -; 1.
DR InterPro; IPR007702; Janus.
DR InterPro; IPR038596; Janus_sf.
DR InterPro; IPR028441; PHPT1.
DR PANTHER; PTHR12258; PTHR12258; 1.
DR PANTHER; PTHR12258:SF10; PTHR12258:SF10; 1.
DR Pfam; PF05005; Ocnus; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Hydrolase; Protein phosphatase; Reference proteome.
FT CHAIN 1..124
FT /note="14 kDa phosphohistidine phosphatase"
FT /id="PRO_0000206153"
FT ACT_SITE 52
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q9NRX4"
FT BINDING 20
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9NRX4"
FT BINDING 93..95
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:Q9NRX4"
SQ SEQUENCE 124 AA; 13997 MW; 583F9A3CE95A2F66 CRC64;
MAADLGQIPD VDIDSDGVFK YVLIRVHLAE PSGDPAKECK EIVRGYKWAE YHADIYDKVS
GELQRNGYDC ECLGGGRISH QSQDRKIHVY GYSMGYGRAQ HSVSTEKIKA KYPDYEVTWA
DDGY
