ID   PHP14_PONAB             Reviewed;         125 AA.
AC   Q5R8L6; Q5R855;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=14 kDa phosphohistidine phosphatase;
DE            EC=3.9.1.3 {ECO:0000250|UniProtKB:Q9NRX4};
DE   AltName: Full=Phosphohistidine phosphatase 1;
DE            Short=PHPT1;
DE   AltName: Full=Protein histidine phosphatase;
DE            Short=PHP;
GN   Name=PHPT1; Synonyms=PHP14;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Heart, and Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Exhibits phosphohistidine phosphatase activity.
CC       {ECO:0000250|UniProtKB:Q9NRX4}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N(pros)-phospho-L-histidyl-[protein] = L-histidyl-
CC         [protein] + phosphate; Xref=Rhea:RHEA:47964, Rhea:RHEA-COMP:9745,
CC         Rhea:RHEA-COMP:9746, ChEBI:CHEBI:15377, ChEBI:CHEBI:29979,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:64837; EC=3.9.1.3;
CC         Evidence={ECO:0000250|UniProtKB:Q9NRX4};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N(tele)-phospho-L-histidyl-[protein] = L-histidyl-
CC         [protein] + phosphate; Xref=Rhea:RHEA:47960, Rhea:RHEA-COMP:9745,
CC         Rhea:RHEA-COMP:10719, ChEBI:CHEBI:15377, ChEBI:CHEBI:29979,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:83586; EC=3.9.1.3;
CC         Evidence={ECO:0000250|UniProtKB:Q9NRX4};
CC   -!- SUBUNIT: Monomer. {ECO:0000250|UniProtKB:Q9NRX4}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q5R8L6-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q5R8L6-2; Sequence=VSP_021108;
CC   -!- SIMILARITY: Belongs to the janus family. {ECO:0000305}.
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DR   EMBL; CR859736; CAH91894.1; -; mRNA.
DR   EMBL; CR859899; CAH92055.1; -; mRNA.
DR   RefSeq; NP_001127500.1; NM_001134028.1. [Q5R8L6-2]
DR   RefSeq; NP_001128837.1; NM_001135365.1. [Q5R8L6-1]
DR   AlphaFoldDB; Q5R8L6; -.
DR   SMR; Q5R8L6; -.
DR   STRING; 9601.ENSPPYP00000022187; -.
DR   PRIDE; Q5R8L6; -.
DR   GeneID; 100174576; -.
DR   GeneID; 100189753; -.
DR   KEGG; pon:100174576; -.
DR   CTD; 29085; -.
DR   eggNOG; ENOG502S4DR; Eukaryota.
DR   InParanoid; Q5R8L6; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005829; C:cytosol; IEA:InterPro.
DR   GO; GO:0101006; F:protein histidine phosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0035971; P:peptidyl-histidine dephosphorylation; IEA:InterPro.
DR   Gene3D; 3.50.20.20; -; 1.
DR   InterPro; IPR007702; Janus.
DR   InterPro; IPR038596; Janus_sf.
DR   InterPro; IPR028441; PHPT1.
DR   PANTHER; PTHR12258; PTHR12258; 1.
DR   PANTHER; PTHR12258:SF10; PTHR12258:SF10; 1.
DR   Pfam; PF05005; Ocnus; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Alternative splicing; Cytoplasm; Hydrolase;
KW   Protein phosphatase; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NRX4"
FT   CHAIN           2..125
FT                   /note="14 kDa phosphohistidine phosphatase"
FT                   /id="PRO_0000253632"
FT   ACT_SITE        53
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NRX4"
FT   BINDING         21
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NRX4"
FT   BINDING         94..96
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NRX4"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NRX4"
FT   VAR_SEQ         94..125
FT                   /note="TMAYGPAQHAISTEKIKAKYPDYEVTWANDGY -> SMMRPTCVPLGASGPR
FT                   IHHQGLWSCPTRHFN (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.1"
FT                   /id="VSP_021108"
SQ   SEQUENCE   125 AA;  13860 MW;  CE3FEB9C2272FC3A CRC64;
     MAAADLAHIP DVDIDSDGVF KYVLIRVHSA SRSGAPVAES KEIVRGYKWA EYHADIYDKV
     SGDMQKQGCD CECLGGGRIS HQSQDKKIHV YGYTMAYGPA QHAISTEKIK AKYPDYEVTW
     ANDGY