ID   PHR_METTH               Reviewed;         445 AA.
AC   P12769; Q50792;
DT   01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   03-AUG-2022, entry version 136.
DE   RecName: Full=Deoxyribodipyrimidine photo-lyase;
DE            EC=4.1.99.3;
DE   AltName: Full=DNA photolyase;
DE   AltName: Full=Photoreactivating enzyme;
GN   Name=phr; OrderedLocusNames=MTH_904;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=7813451; DOI=10.1002/j.1460-2075.1994.tb06961.x;
RA   Yasui A., Eker A.P., Yasuhira S., Yajima H., Kobayashi T., Takao M.,
RA   Oikawa A.;
RT   "A new class of DNA photolyases present in various organisms including
RT   aplacental mammals.";
RL   EMBO J. 13:6143-6151(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
CC   -!- FUNCTION: Involved in repair of UV radiation-induced DNA damage.
CC       Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl
CC       pyrimidine dimers (in cis-syn configuration), which are formed between
CC       adjacent bases on the same DNA strand upon exposure to ultraviolet
CC       radiation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in
CC         DNA).; EC=4.1.99.3;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC       Note=Binds 1 FAD per subunit.;
CC   -!- COFACTOR:
CC       Name=coenzyme F420-(gamma-Glu)n; Xref=ChEBI:CHEBI:133980;
CC       Note=Binds 1 coenzyme F420 non-covalently per subunit.;
CC   -!- SIMILARITY: Belongs to the DNA photolyase class-2 family.
CC       {ECO:0000305}.
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DR   EMBL; D30752; BAA06411.1; -; Genomic_DNA.
DR   EMBL; AE000666; AAB85402.1; -; Genomic_DNA.
DR   PIR; A69221; A69221.
DR   RefSeq; WP_010876537.1; NC_000916.1.
DR   AlphaFoldDB; P12769; -.
DR   SMR; P12769; -.
DR   STRING; 187420.MTH_904; -.
DR   EnsemblBacteria; AAB85402; AAB85402; MTH_904.
DR   GeneID; 1471312; -.
DR   KEGG; mth:MTH_904; -.
DR   PATRIC; fig|187420.15.peg.889; -.
DR   HOGENOM; CLU_026342_2_1_2; -.
DR   OMA; RHLGFML; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0003904; F:deoxyribodipyrimidine photo-lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR036134; Crypto/Photolyase_FAD-like_sf.
DR   InterPro; IPR036155; Crypto/Photolyase_N_sf.
DR   InterPro; IPR008148; DNA_photolyase_2.
DR   InterPro; IPR032673; DNA_photolyase_2_CS.
DR   InterPro; IPR006050; DNA_photolyase_N.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Pfam; PF00875; DNA_photolyase; 1.
DR   SUPFAM; SSF48173; SSF48173; 1.
DR   SUPFAM; SSF52425; SSF52425; 1.
DR   TIGRFAMs; TIGR00591; phr2; 1.
DR   PROSITE; PS01083; DNA_PHOTOLYASES_2_1; 1.
DR   PROSITE; PS01084; DNA_PHOTOLYASES_2_2; 1.
DR   PROSITE; PS51645; PHR_CRY_ALPHA_BETA; 1.
PE   3: Inferred from homology;
KW   Chromophore; DNA damage; DNA repair; DNA-binding; FAD; Flavoprotein; Lyase;
KW   Reference proteome.
FT   CHAIN           1..445
FT                   /note="Deoxyribodipyrimidine photo-lyase"
FT                   /id="PRO_0000085117"
FT   DOMAIN          20..148
FT                   /note="Photolyase/cryptochrome alpha/beta"
FT   BINDING         239
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   445 AA;  51762 MW;  2D610370798F8C81 CRC64;
     MIHAERIRNL NGEEPDLRGS YVVYWMQASV RSHWNHALEY AIETANSLKK PLIVVFGLTD
     DFPNANSRHY RFLIEGLRDV RSNLRERGIQ LVVERDSPPS VLLKYADDAA AAVTDRGYLD
     IQKEWVDEAA GALHIPLTQV ESNVIVPVET ASDKEEYSAG TFKPKIKRHL KRFMVPLRMR
     TLKMDSLDLE PGPEFEDAVR DFRAPEDLEP SVFRGGTSTA LSIFSEFLRE KLECFERYRN
     DPVKNCLSNM SPYLHFGQIS PLYLALRASE AGECPEFLEE LIVRRELSMN FVHYSDSYSS
     ISCLPEWAQR TLMDHVADPR EYEYSLRELE SASTHDPYWN AAQQEMVITG KMHGYMRMYW
     GKKILEWTDH PARAYDIALY LNDRYEIDGR DPNGFAGVAW CFGKHDRAWA EREIFGKVRY
     MNDRGLKRKF RIDEYVDRIR GLMDE