ID   PHR_METTM               Reviewed;         444 AA.
AC   P58818; D9PXD8;
DT   10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   30-NOV-2010, sequence version 2.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Deoxyribodipyrimidine photo-lyase;
DE            EC=4.1.99.3;
DE   AltName: Full=DNA photolyase;
DE   AltName: Full=Photoreactivating enzyme;
GN   Name=phr; OrderedLocusNames=MTBMA_c12980;
OS   Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM
OS   14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium
OS   thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=79929;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 /
RC   Marburg;
RX   PubMed=20802048; DOI=10.1128/jb.00844-10;
RA   Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H.,
RA   Gottschalk G., Thauer R.K.;
RT   "Complete genome sequence of Methanothermobacter marburgensis, a
RT   methanoarchaeon model organism.";
RL   J. Bacteriol. 192:5850-5851(2010).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-28.
RC   STRAIN=ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 /
RC   Marburg;
RX   PubMed=2668276; DOI=10.1016/s0021-9258(18)80082-5;
RA   Kiener A., Husain I., Sancar A., Walsh C.;
RT   "Purification and properties of Methanobacterium thermoautotrophicum DNA
RT   photolyase.";
RL   J. Biol. Chem. 264:13880-13887(1989).
CC   -!- FUNCTION: Involved in repair of UV radiation-induced DNA damage.
CC       Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl
CC       pyrimidine dimers (in cis-syn configuration), which are formed between
CC       adjacent bases on the same DNA strand upon exposure to ultraviolet
CC       radiation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in
CC         DNA).; EC=4.1.99.3;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC       Note=Binds 1 FAD. The FAD is in its reduced state.;
CC   -!- COFACTOR:
CC       Name=coenzyme F420-(gamma-Glu)n; Xref=ChEBI:CHEBI:133980;
CC       Note=Binds 1 F420 non-covalently per subunit. The F420 is in its
CC       oxidized state.;
CC   -!- SIMILARITY: Belongs to the DNA photolyase class-2 family.
CC       {ECO:0000305}.
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DR   EMBL; CP001710; ADL58886.1; -; Genomic_DNA.
DR   RefSeq; WP_013296108.1; NC_014408.1.
DR   AlphaFoldDB; P58818; -.
DR   SMR; P58818; -.
DR   STRING; 79929.MTBMA_c12980; -.
DR   EnsemblBacteria; ADL58886; ADL58886; MTBMA_c12980.
DR   GeneID; 9705006; -.
DR   KEGG; mmg:MTBMA_c12980; -.
DR   PATRIC; fig|79929.8.peg.1262; -.
DR   HOGENOM; CLU_026342_2_1_2; -.
DR   OMA; RHLGFML; -.
DR   OrthoDB; 15287at2157; -.
DR   Proteomes; UP000000345; Chromosome.
DR   GO; GO:0003904; F:deoxyribodipyrimidine photo-lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR036134; Crypto/Photolyase_FAD-like_sf.
DR   InterPro; IPR036155; Crypto/Photolyase_N_sf.
DR   InterPro; IPR008148; DNA_photolyase_2.
DR   InterPro; IPR032673; DNA_photolyase_2_CS.
DR   InterPro; IPR006050; DNA_photolyase_N.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Pfam; PF00875; DNA_photolyase; 1.
DR   SUPFAM; SSF48173; SSF48173; 1.
DR   SUPFAM; SSF52425; SSF52425; 1.
DR   TIGRFAMs; TIGR00591; phr2; 1.
DR   PROSITE; PS01083; DNA_PHOTOLYASES_2_1; 1.
DR   PROSITE; PS01084; DNA_PHOTOLYASES_2_2; 1.
DR   PROSITE; PS51645; PHR_CRY_ALPHA_BETA; 1.
PE   1: Evidence at protein level;
KW   Chromophore; Direct protein sequencing; DNA damage; DNA repair;
KW   DNA-binding; FAD; Flavoprotein; Lyase.
FT   CHAIN           1..444
FT                   /note="Deoxyribodipyrimidine photo-lyase"
FT                   /id="PRO_0000085118"
FT   DOMAIN          20..148
FT                   /note="Photolyase/cryptochrome alpha/beta"
FT   BINDING         239
FT                   /ligand="DNA"
FT                   /ligand_id="ChEBI:CHEBI:16991"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   444 AA;  51888 MW;  442143277F21894D CRC64;
     MIHDERIRSL NTEKPARDGK YVIYWMQASV RAHWNHALEY AIETANSLHK PLIVIFGLTD
     EFPNANSRHY RFLIEGLRDV GDALMKRGAR LVVENERPPS AVMRYSDEAS AVVVDRGYLD
     IQKEWVDELA ESLHVPLMQV ESNVIVPVET ASPKEEYSAG TFRPKITREL ERFMVPLKER
     RLAVDSLDLD PGPDLNDVTG KFRASEELEP SIFSGGASEA IRLFDEFLSE KLACFEKYRN
     DPVKNCLSNM SPYLHFGHIS PLYLAMKASK TGECPEFLEE LIVRRELSMN FVHYSDNYSS
     IRCLPEWAQK TLMEHARDPK EYEYTLREFE EARTHDPYWN AAQKEMVITG KMHGYMRMYW
     GKKILEWTDH PERAYDIAIY LNDKYEIDGR DPNGFTGVAW CFGKHDRAWA EREIFGKVRY
     MNDRGLERKF RIGEYVRRVQ ELEE