PHS1_MAIZE
ID PHS1_MAIZE Reviewed; 347 AA.
AC Q6W2J0;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=Protein POOR HOMOLOGOUS SYNAPSIS 1 {ECO:0000303|PubMed:14704428};
GN Name=PHS1 {ECO:0000303|PubMed:14704428};
GN ORFNames=ZEAMMB73_074178 {ECO:0000312|EMBL:AFW86601.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. B73;
RX PubMed=14704428; DOI=10.1126/science.1091110;
RA Pawlowski W.P., Golubovskaya I.N., Timofejeva L., Meeley R.B.,
RA Sheridan W.F., Cande W.Z.;
RT "Coordination of meiotic recombination, pairing, and synapsis by PHS1.";
RL Science 303:89-92(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RX PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA Walbot V., Yu Y.;
RT "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL PLoS Genet. 5:E1000740-E1000740(2009).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS OF ARG-148.
RX PubMed=19918061; DOI=10.1073/pnas.0906273106;
RA Ronceret A., Doutriaux M.P., Golubovskaya I.N., Pawlowski W.P.;
RT "PHS1 regulates meiotic recombination and homologous chromosome pairing by
RT controlling the transport of RAD50 to the nucleus.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:20121-20126(2009).
CC -!- FUNCTION: Required for accurate chromosome segregation in meiosis.
CC Required for pairing to occur between homologous chromosomes. Acts in
CC early recombination steps and ensures pairing fidelity and proper
CC repair of meiotic DNA double-strand-breaks (PubMed:14704428,
CC PubMed:19918061). Regulates recombination and pairing of homologous
CC chromosomes during meiotic prophase by controlling transport of RAD50
CC from cytoplasm to the nucleus. May affect pairing of the gene-rich
CC fraction of the genome rather than preventing pairing between
CC repetitive DNA elements (PubMed:19918061).
CC {ECO:0000269|PubMed:14704428, ECO:0000269|PubMed:19918061}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19918061}.
CC Note=Localizes to the cytoplasm during leptotene, zygotene and
CC pachytene. {ECO:0000269|PubMed:19918061}.
CC -!- DISRUPTION PHENOTYPE: Male and female sterility. During meiosis,
CC synapsis is uncoupled from recombination and pairing in the mutant
CC gametes. {ECO:0000269|PubMed:14704428}.
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DR EMBL; AY316742; AAQ84720.1; -; Genomic_DNA.
DR EMBL; CM000785; AFW86601.1; -; Genomic_DNA.
DR EMBL; BT041980; ACF86985.1; -; mRNA.
DR RefSeq; NP_001141750.1; NM_001148278.1.
DR AlphaFoldDB; Q6W2J0; -.
DR STRING; 4577.GRMZM2G100103_P01; -.
DR PaxDb; Q6W2J0; -.
DR EnsemblPlants; Zm00001eb382430_T004; Zm00001eb382430_P004; Zm00001eb382430.
DR GeneID; 100273885; -.
DR Gramene; Zm00001eb382430_T004; Zm00001eb382430_P004; Zm00001eb382430.
DR KEGG; zma:100273885; -.
DR eggNOG; ENOG502RYMD; Eukaryota.
DR HOGENOM; CLU_044780_0_0_1; -.
DR OrthoDB; 1377750at2759; -.
DR Proteomes; UP000007305; Chromosome 9.
DR ExpressionAtlas; Q6W2J0; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0007129; P:homologous chromosome pairing at meiosis; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Cytoplasm; Meiosis; Reference proteome.
FT CHAIN 1..347
FT /note="Protein POOR HOMOLOGOUS SYNAPSIS 1"
FT /id="PRO_0000435707"
FT MUTAGEN 148
FT /note="R->H: Meiotic sterility."
FT /evidence="ECO:0000269|PubMed:19918061"
SQ SEQUENCE 347 AA; 38258 MW; A85DBB63700FB619 CRC64;
MADAADSSMA LVHSSLADSV LTSPRTLRQG QKWEVEYARY FGTPRRDPTA APPSGLRYIM
RGVHRHQGTW IPASCPASLC VCHPSLPSAV PVLTISIGDV VFEEHFVSIL NFSWPQVTCV
TQCPIRGSRV VFVSFCDKFK QIQKFAVRFP QPCDAESFLS CVECSCGSSG TMDIIPFGSD
YVCEDSSASE YIVSNGLHHR LDDASNLEEQ CFDHTIDEPP MNYHEETDQH VLEPLSASNT
SNNSAFPPSF NQMLKSCSID YDQEEPCPLA ASNHVLQEVY VLDTSHDVAN EERTAGKGMD
AAEGVDASIL TYDLMARIKT YMADESFNDM LLKLDKAIDE LGGDMSL
