PHSB_ARATH
ID PHSB_ARATH Reviewed; 495 AA.
AC Q9C6S5; Q3ED40;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Probable polyamine transporter At1g31830;
GN OrderedLocusNames=At1g31830; ORFNames=F5M6.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable cell membrane polyamine/proton symporter involved in
CC the polyamine uptake in cells. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9C6S5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9C6S5-2; Sequence=VSP_044089;
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Polyamine:cation symporter (PHS) (TC 2.A.3.12) family.
CC {ECO:0000305}.
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DR EMBL; AC079041; AAG50709.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31400.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE31401.1; -; Genomic_DNA.
DR EMBL; AK317082; BAH19774.1; -; mRNA.
DR EMBL; BT030375; ABO38788.1; -; mRNA.
DR PIR; D86442; D86442.
DR RefSeq; NP_174466.1; NM_102920.3. [Q9C6S5-1]
DR RefSeq; NP_973947.1; NM_202218.3. [Q9C6S5-2]
DR AlphaFoldDB; Q9C6S5; -.
DR SMR; Q9C6S5; -.
DR BioGRID; 25307; 1.
DR IntAct; Q9C6S5; 1.
DR STRING; 3702.AT1G31830.1; -.
DR PaxDb; Q9C6S5; -.
DR PRIDE; Q9C6S5; -.
DR ProteomicsDB; 236156; -. [Q9C6S5-1]
DR EnsemblPlants; AT1G31830.1; AT1G31830.1; AT1G31830. [Q9C6S5-1]
DR EnsemblPlants; AT1G31830.2; AT1G31830.2; AT1G31830. [Q9C6S5-2]
DR GeneID; 840073; -.
DR Gramene; AT1G31830.1; AT1G31830.1; AT1G31830. [Q9C6S5-1]
DR Gramene; AT1G31830.2; AT1G31830.2; AT1G31830. [Q9C6S5-2]
DR KEGG; ath:AT1G31830; -.
DR Araport; AT1G31830; -.
DR TAIR; locus:2034451; AT1G31830.
DR eggNOG; KOG1287; Eukaryota.
DR InParanoid; Q9C6S5; -.
DR OrthoDB; 621852at2759; -.
DR PhylomeDB; Q9C6S5; -.
DR PRO; PR:Q9C6S5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C6S5; baseline and differential.
DR Genevisible; Q9C6S5; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015203; F:polyamine transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0015846; P:polyamine transport; IDA:TAIR.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR044566; RMV1-like.
DR PANTHER; PTHR45826; PTHR45826; 1.
DR Pfam; PF13520; AA_permease_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..495
FT /note="Probable polyamine transporter At1g31830"
FT /id="PRO_0000418909"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 380..400
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..437
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..16
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:19423640"
FT /id="VSP_044089"
SQ SEQUENCE 495 AA; 54828 MW; D376E0AF27616088 CRC64;
MQKRRIITVN PSASIEMSQY ENNEVPYSSV GADEVPSSPP KATDKIRKVS MLPLVFLIFY
EVSGGPFGVE DSVNAAGPLL ALLGFVIFPF IWSIPEALIT AEMGTMYPEN GGYVVWVSSA
LGPFWGFQQG WMKWLSGVID NALYPVLFLD YLKSGVPALG SGLPRVASIL VLTILLTYLN
YRGLTIVGWV AVLMGVFSIL PFAVMGLISI PQLEPSRWLV MDLGNVNWNL YLNTLFWNLN
YWDSISTLAG EVENPNHTLP KALFYGVILV ACSYIFPLLA GIGAIPLERE KWTDGYFSDV
AKALGGAWLR WWVQAAAATS NMGMFIAEMS SDSFQLLGMA ERGMLPEFFA KRSRYGTPLL
GILFSASGVV LLSWLSFQEI VAAENLLYCV GMILEFIAFV RMRMKHPAAS RPYKIPIGTT
GSILMCIPPT ILICAVVALS SLKVAAVSIV MMIIGFLIHP LLNHMDRKRW VKFSISSDLP
DLQQQTREYE ETLIR
