PHS_YEAST
ID PHS_YEAST Reviewed; 120 AA.
AC P38744; D3DKR5;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Putative pterin-4-alpha-carbinolamine dehydratase;
DE Short=PHS;
DE EC=4.2.1.96;
DE AltName: Full=4-alpha-hydroxy-tetrahydropterin dehydratase;
DE AltName: Full=Pterin carbinolamine dehydratase;
DE Short=PCD;
GN OrderedLocusNames=YHL018W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(4aS,6R)-4a-hydroxy-L-erythro-5,6,7,8-tetrahydrobiopterin =
CC (6R)-L-erythro-6,7-dihydrobiopterin + H2O; Xref=Rhea:RHEA:11920,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15642, ChEBI:CHEBI:43120; EC=4.2.1.96;
CC -!- MISCELLANEOUS: Present with 752 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the pterin-4-alpha-carbinolamine dehydratase
CC family. {ECO:0000305}.
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DR EMBL; U11582; AAB65071.1; -; Genomic_DNA.
DR EMBL; AY558391; AAS56717.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06667.1; -; Genomic_DNA.
DR PIR; S46832; S46832.
DR RefSeq; NP_011845.1; NM_001179098.1.
DR AlphaFoldDB; P38744; -.
DR SMR; P38744; -.
DR BioGRID; 36405; 82.
DR DIP; DIP-1872N; -.
DR IntAct; P38744; 12.
DR MINT; P38744; -.
DR STRING; 4932.YHL018W; -.
DR MaxQB; P38744; -.
DR PaxDb; P38744; -.
DR PRIDE; P38744; -.
DR EnsemblFungi; YHL018W_mRNA; YHL018W; YHL018W.
DR GeneID; 856368; -.
DR KEGG; sce:YHL018W; -.
DR SGD; S000001010; YHL018W.
DR VEuPathDB; FungiDB:YHL018W; -.
DR eggNOG; KOG4073; Eukaryota.
DR HOGENOM; CLU_081974_4_0_1; -.
DR InParanoid; P38744; -.
DR OMA; IAMRSHL; -.
DR BioCyc; YEAST:G3O-31038-MON; -.
DR Reactome; R-SCE-8964208; Phenylalanine metabolism.
DR PRO; PR:P38744; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38744; protein.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0008124; F:4-alpha-hydroxytetrahydrobiopterin dehydratase activity; IBA:GO_Central.
DR GO; GO:0006729; P:tetrahydrobiopterin biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1360.20; -; 1.
DR InterPro; IPR036428; PCD_sf.
DR InterPro; IPR001533; Pterin_deHydtase.
DR PANTHER; PTHR12599; PTHR12599; 1.
DR Pfam; PF01329; Pterin_4a; 1.
DR SUPFAM; SSF55248; SSF55248; 1.
PE 1: Evidence at protein level;
KW Lyase; Reference proteome.
FT CHAIN 1..120
FT /note="Putative pterin-4-alpha-carbinolamine dehydratase"
FT /id="PRO_0000063064"
SQ SEQUENCE 120 AA; 14026 MW; 4265E895BD5CDB02 CRC64;
MHNKIVRIAS SALTGGKLLE KLKPLTRWEV QWDPNKTKCL GITREVTFKD YETTWAFLTR
VSMRSHLWGH HPLIHTSYTW VKLELHTHDI DPKDGAHSQL SDIDVRMAKR IDSYIDEMTT
