PHT11_ORYSJ
ID PHT11_ORYSJ Reviewed; 527 AA.
AC Q8H6H4; A0A0P0VT26; Q56UU2;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Inorganic phosphate transporter 1-1;
DE Short=OsPT1;
DE Short=OsPht1;1;
DE AltName: Full=H(+)/Pi cotransporter;
GN Name=PHT1-1; Synonyms=PT1; OrderedLocusNames=Os03g0150600, LOC_Os03g05620;
GN ORFNames=OsJ_009086;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=12271140; DOI=10.1073/pnas.202474599;
RA Paszkowski U., Kroken S., Roux C., Briggs S.P.;
RT "Rice phosphate transporters include an evolutionarily divergent gene
RT specifically activated in arbuscular mycorrhizal symbiosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13324-13329(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Dongjin;
RA Baek S.-H., Kim H.-S., Yun S.J.;
RT "Regulation of rice phosphate transporter gene expression by phosphate
RT availability.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=18563580; DOI=10.1007/s10529-008-9757-7;
RA Seo H.-M., Jung Y., Song S., Kim Y., Kwon T., Kim D.-H., Jeung S.-J.,
RA Yi Y.-B., Yi G., Nam M.-H., Nam J.;
RT "Increased expression of OsPT1, a high-affinity phosphate transporter,
RT enhances phosphate acquisition in rice.";
RL Biotechnol. Lett. 30:1833-1838(2008).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate
CC (By similarity). Required for phosphate acquisition in plant.
CC {ECO:0000250, ECO:0000269|PubMed:18563580}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8H6H4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8H6H4-2; Sequence=VSP_039470;
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems and leaves.
CC {ECO:0000269|PubMed:18563580}.
CC -!- INDUCTION: In roots by phosphate starvation.
CC {ECO:0000269|PubMed:18563580}.
CC -!- MISCELLANEOUS: Plants overexpressing PHT1-1 accumulates almost twice
CC the amount of phosphate in the shoots compared to the wild-type plants.
CC Although related to the sugar transporter family, it does not transport
CC sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; AF536961; AAN39042.1; -; Genomic_DNA.
DR EMBL; AY569607; AAU84426.1; -; mRNA.
DR EMBL; DP000009; ABF94006.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10891.2; -; Genomic_DNA.
DR EMBL; AP014959; BAS82317.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82318.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ25603.1; -; Genomic_DNA.
DR EMBL; AK065075; -; NOT_ANNOTATED_CDS; mRNA.
DR RefSeq; XP_015631295.1; XM_015775809.1. [Q8H6H4-1]
DR AlphaFoldDB; Q8H6H4; -.
DR SMR; Q8H6H4; -.
DR STRING; 4530.OS03T0150600-01; -.
DR PaxDb; Q8H6H4; -.
DR PRIDE; Q8H6H4; -.
DR EnsemblPlants; Os03t0150600-01; Os03t0150600-01; Os03g0150600. [Q8H6H4-1]
DR EnsemblPlants; Os03t0150600-02; Os03t0150600-02; Os03g0150600. [Q8H6H4-2]
DR GeneID; 4331635; -.
DR Gramene; Os03t0150600-01; Os03t0150600-01; Os03g0150600. [Q8H6H4-1]
DR Gramene; Os03t0150600-02; Os03t0150600-02; Os03g0150600. [Q8H6H4-2]
DR KEGG; osa:4331635; -.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR OMA; TRGHHTG; -.
DR OrthoDB; 762280at2759; -.
DR PlantReactome; R-OSA-9618218; Arsenic uptake and detoxification.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q8H6H4; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004738; Phos_permease.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00887; 2A0109; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Phosphate transport; Reference proteome;
KW Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..527
FT /note="Inorganic phosphate transporter 1-1"
FT /id="PRO_0000365480"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..124
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..211
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..348
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..405
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 427..442
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 443..463
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 464..481
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 503..527
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VAR_SEQ 444..479
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_039470"
FT CONFLICT 12
FT /note="L -> R (in Ref. 2; AAU84426)"
FT /evidence="ECO:0000305"
FT CONFLICT 402
FT /note="H -> R (in Ref. 8; AK065075)"
FT /evidence="ECO:0000305"
FT CONFLICT 506
FT /note="M -> T (in Ref. 2; AAU84426)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 57458 MW; 84DC56E1AD0728CE CRC64;
MAGGQLNVLS TLDQAKTQWY HFMAIVIAGM GFFTDAYDLF CISLVTKLLG RIYYTDDSKD
TPGALPPNVS AAVTGVALCG TLAGQLFFGW LGDKLGRKSV YGFTLILMVV CSVASGLSFG
SSAKGVVSTL CFFRFWLGFG IGGDYPLSAT IMSEYANKRT RGAFIAAVFA MQGFGILFGA
IVALAVSAGF RHAYPAPSYS DNHAASLVPQ ADYVWRIILM FGTVPAALTY YWRMKMPETA
RYTALIARNA KQAAADMSKV LHTQIEESAD RAETVAVGGE SWGLFSRQFL RRHGLHLLAT
TSTWFLLDIA FYSQNLFQKD IFSKVGWIPP AKTMNALEEL YRIARAQALI ALCGTIPGYW
FTVAFIEIMG RFWIQIMGFA MMTAFMLGLA IPYHHWTTPG HHTGFIVMYG FTFFFANFGP
NSTTFIVPAE IYPARLRSTC HGISAAAGKA GAIIGAFGFL YAAQDQHKPE PGYPRGIGIK
NALFVLAGTN FLGTIMTLLV PESKGMSLEV ISQEVADGDD EEAAYPK
