PHT12_ARATH
ID PHT12_ARATH Reviewed; 524 AA.
AC Q96243; O48640;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Probable inorganic phosphate transporter 1-2;
DE Short=AtPht1;2;
DE AltName: Full=H(+)/Pi cotransporter;
GN Name=PHT1-2; Synonyms=APT1, PHT2; OrderedLocusNames=At5g43370;
GN ORFNames=MWF20.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Columbia; TISSUE=Root;
RX PubMed=9025304; DOI=10.1046/j.1365-313x.1997.11010083.x;
RA Smith F.W., Ealing P.M., Dong B., Delhaize E.;
RT "The cloning of two Arabidopsis genes belonging to a phosphate transporter
RT family.";
RL Plant J. 11:83-92(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Mitsukawa N., Okumura S., Shibata D.;
RT "High-affinity phosphate transporter genes of Arabidopsis thaliana.";
RL Soil Sci. Plant Nutr. 43:971-974(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP INDUCTION, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12164813; DOI=10.1046/j.1365-313x.2002.01356.x;
RA Mudge S.R., Rae A.L., Diatloff E., Smith F.W.;
RT "Expression analysis suggests novel roles for members of the Pht1 family of
RT phosphate transporters in Arabidopsis.";
RL Plant J. 31:341-353(2002).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Root specific, especially in trichoblasts. In
CC mature plants, localized in root cortical cells and young lateral
CC roots. {ECO:0000269|PubMed:12164813, ECO:0000269|PubMed:9025304}.
CC -!- INDUCTION: In roots by phosphate starvation.
CC {ECO:0000269|PubMed:12164813, ECO:0000269|PubMed:9025304}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; Y07681; CAA68945.1; -; Genomic_DNA.
DR EMBL; AB000094; BAA24282.1; -; Genomic_DNA.
DR EMBL; AB025638; BAA97416.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94950.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94951.1; -; Genomic_DNA.
DR RefSeq; NP_001190462.1; NM_001203533.1.
DR RefSeq; NP_199151.1; NM_123703.3.
DR AlphaFoldDB; Q96243; -.
DR SMR; Q96243; -.
DR STRING; 3702.AT5G43370.2; -.
DR PaxDb; Q96243; -.
DR PRIDE; Q96243; -.
DR ProteomicsDB; 234751; -.
DR EnsemblPlants; AT5G43370.1; AT5G43370.1; AT5G43370.
DR EnsemblPlants; AT5G43370.2; AT5G43370.2; AT5G43370.
DR GeneID; 834355; -.
DR Gramene; AT5G43370.1; AT5G43370.1; AT5G43370.
DR Gramene; AT5G43370.2; AT5G43370.2; AT5G43370.
DR KEGG; ath:AT5G43370; -.
DR Araport; AT5G43370; -.
DR TAIR; locus:2176481; AT5G43370.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; Q96243; -.
DR OMA; IVWFCMA; -.
DR OrthoDB; 762280at2759; -.
DR PhylomeDB; Q96243; -.
DR PRO; PR:Q96243; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q96243; baseline and differential.
DR Genevisible; Q96243; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IEP:TAIR.
DR GO; GO:0006817; P:phosphate ion transport; TAS:TAIR.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004738; Phos_permease.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00887; 2A0109; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphate transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..524
FT /note="Probable inorganic phosphate transporter 1-2"
FT /id="PRO_0000050469"
FT TOPO_DOM 1..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..131
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..211
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..348
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..402
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..484
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 506..524
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 418
FT /note="F -> L (in Ref. 1; CAA68945)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 524 AA; 57644 MW; 54664D4CD3ABE9D7 CRC64;
MAEQQLGVLK ALDVAKTQLY HFTAIVIAGM GFFTDAYDLF CVSLVTKLLG RIYYFNPESA
KPGSLPPHVA AAVNGVALCG TLSGQLFFGW LGDKLGRKKV YGLTLIMMIL CSVASGLSFG
NEAKGVMTTL CFFRFWLGFG IGGDYPLSAT IMSEYANKKT RGAFIAAVFA MQGVGILAGG
FVALAVSSIF DKKFPAPTYA VNRALSTPPQ VDYIWRIIVM FGALPAALTY YWRMKMPETA
RYTALVAKNI KQATADMSKV LQTDIELEER VEDDVKDPRQ NYGLFSKEFL RRHGLHLLGT
TSTWFLLDIA FYSQNLFQKD IFSAIGWIPK AATMNATHEV FRIARAQTLI ALCSTVPGYW
FTVAFIDTIG RFKIQLNGFF MMTVFMFAIA FPYNHWIKPE NRIGFVVMYS LTFFFANFGP
NATTFIVPAE IFPARLRSTC HGISAAAGKA GAIIGAFGFL YAAQNQDKAK VDAGYPPGIG
VKNSLIVLGV LNFIGMLFTF LVPEPKGKSL EELSGEAEVS HDEK
