PHT12_ORYSJ
ID PHT12_ORYSJ Reviewed; 528 AA.
AC Q8GSD9; A0A0N7KGL1; Q56UU1;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Inorganic phosphate transporter 1-2;
DE Short=OsPT2;
DE Short=OsPht1;2;
DE AltName: Full=H(+)/Pi cotransporter;
DE AltName: Full=OsPT1;
GN Name=PTH1-2; Synonyms=PT1, PT2;
GN OrderedLocusNames=Os03g0150800, LOC_Os03g05640; ORFNames=OsJ_009088;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND INDUCTION.
RX PubMed=12271140; DOI=10.1073/pnas.202474599;
RA Paszkowski U., Kroken S., Roux C., Briggs S.P.;
RT "Rice phosphate transporters include an evolutionarily divergent gene
RT specifically activated in arbuscular mycorrhizal symbiosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13324-13329(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yao Q., Peng R., Xiong A.;
RT "Isolation of a phosphate transporter gene from rice.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Dongjin;
RA Baek S.-H., Kim H.-S., Yun S.J.;
RT "Regulation of rice phosphate transporter gene expression by phosphate
RT availability.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [7]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [9]
RP INDUCTION.
RX PubMed=18563580; DOI=10.1007/s10529-008-9757-7;
RA Seo H.-M., Jung Y., Song S., Kim Y., Kwon T., Kim D.-H., Jeung S.-J.,
RA Yi Y.-B., Yi G., Nam M.-H., Nam J.;
RT "Increased expression of OsPT1, a high-affinity phosphate transporter,
RT enhances phosphate acquisition in rice.";
RL Biotechnol. Lett. 30:1833-1838(2008).
RN [10]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=18980647; DOI=10.1111/j.1365-313x.2008.03726.x;
RA Ai P., Sun S., Zhao J., Fan X., Xin W., Guo Q., Yu L., Shen Q., Wu P.,
RA Miller A.J., Xu G.;
RT "Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different
RT functions and kinetic properties in uptake and translocation.";
RL Plant J. 57:798-809(2009).
RN [11]
RP FUNCTION, AND INDUCTION BY PHR2.
RX PubMed=20149131; DOI=10.1111/j.1365-313x.2010.04170.x;
RA Liu F., Wang Z., Ren H., Shen C., Li Y., Ling H.Q., Wu C., Lian X., Wu P.;
RT "OsSPX1 suppresses the function of OsPHR2 in the regulation of expression
RT of OsPT2 and phosphate homeostasis in shoots of rice.";
RL Plant J. 62:508-517(2010).
CC -!- FUNCTION: Low-affinity transporter for inorganic phosphate (Pi)
CC (PubMed:18980647). Involved in internal Pi transport from root to shoot
CC (PubMed:18980647, PubMed:20149131). Responsible for most of the PHR2-
CC mediated accumulation of excess shoot Pi under abundant Pi conditions,
CC but not for PHO2-mediated accumulation of excess shoot Pi
CC (PubMed:20149131). Acts as a H(+):phosphate symporter
CC (PubMed:18980647). {ECO:0000269|PubMed:18980647,
CC ECO:0000269|PubMed:20149131}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in the root stele and leaf phloem and
CC xylem. {ECO:0000269|PubMed:18980647}.
CC -!- INDUCTION: Regulated by the transcription factor PHR2
CC (PubMed:20149131). Up-regulated in roots by phosphate starvation
CC (PubMed:18563580). Down-regulated in roots by high phosphate or
CC colonization by the mycorrhizal fungus G.intraradices
CC (PubMed:12271140). {ECO:0000269|PubMed:12271140,
CC ECO:0000269|PubMed:18563580, ECO:0000269|PubMed:18980647,
CC ECO:0000269|PubMed:20149131}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; AF536959; AAN39040.1; -; mRNA.
DR EMBL; AF536962; AAN39043.1; -; Genomic_DNA.
DR EMBL; AY332471; AAQ01157.1; -; mRNA.
DR EMBL; AY569608; AAU84427.1; -; mRNA.
DR EMBL; DP000009; ABF94008.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10893.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS82321.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ25605.1; -; Genomic_DNA.
DR RefSeq; XP_015630484.1; XM_015774998.1.
DR AlphaFoldDB; Q8GSD9; -.
DR SMR; Q8GSD9; -.
DR STRING; 4530.OS03T0150800-01; -.
DR PaxDb; Q8GSD9; -.
DR PRIDE; Q8GSD9; -.
DR EnsemblPlants; Os03t0150800-01; Os03t0150800-01; Os03g0150800.
DR GeneID; 4331637; -.
DR Gramene; Os03t0150800-01; Os03t0150800-01; Os03g0150800.
DR KEGG; osa:4331637; -.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; Q8GSD9; -.
DR OMA; EHWTKAS; -.
DR OrthoDB; 762280at2759; -.
DR PlantReactome; R-OSA-9031225; Response to phosphate deficiency.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q8GSD9; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004738; Phos_permease.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00887; 2A0109; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphate transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..528
FT /note="Inorganic phosphate transporter 1-2"
FT /id="PRO_0000365481"
FT TOPO_DOM 1..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..71
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 72..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..125
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..296
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 297..317
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 318..348
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..405
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 427..442
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 443..463
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 464..481
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 482..502
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 503..528
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 473
FT /note="Y -> H (in Ref. 3; AAU84427)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 528 AA; 57841 MW; 3160038E0CE12842 CRC64;
MAGSQLNVLV KLDQAKTQWY HFMAIVIAGM GFFTDAYDLF CIALVTKLLG RLYYTDITKP
NPGTLPPNVS SAVTGVALCG TLAGQLFFGW LGDKLGRKSV YGFTLILMVV CSIASGLSFG
HTPKSVIATL CFFRFWLGFG IGGDYPLSAT IMSEYASKKT RGAFIAAVFA MQGFGILFGA
IVALVVSAGF RHAYPAPSYA QNPAASLAPQ ADYTWRLILM FGTIPAGLTY YWRMKMPETA
RYTALVARNA KQAAADMSKV LHAEIEERPE VVESQVVAGE TWGLFSRQFM KRHGMHLLAT
TSTWFLLDIA FYSQNLFQKD IFSKVGWIPP AKTMNALEEL YRISRAQALI ALCGTIPGYW
FTVAFIDIVG RFWIQIMGFF MMTVFMLALG VPYDHWTHPA HHTGFVVLYA LTFFFANFGP
NSTTFIVPAE IFPARLRSTC HGISAASGKA GAIIGAFGFL YAAQDQHNPD AGYSRGIGIR
NALFVLAGTN FLGMLMTLLV PESKGLSLEE MSKDNVVDET AQEAIAQA
