PHT12_PETHY
ID PHT12_PETHY Reviewed; 189 AA.
AC B2CPI4;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=Low affinity inorganic phosphate transporter 2 {ECO:0000303|PubMed:18315538};
DE Short=PhPT2 {ECO:0000303|PubMed:18315538};
DE Short=PhPht1;2 {ECO:0000305};
DE AltName: Full=Arbuscular mycorrhiza-induced phosphate transporter PT2 {ECO:0000305};
DE Short=AM-induced phosphate transporter PT2 {ECO:0000305};
DE AltName: Full=H(+)/Pi cotransporter PT2 {ECO:0000305};
DE Flags: Fragment;
GN Name=PT2 {ECO:0000303|PubMed:18315538};
OS Petunia hybrida (Petunia).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Petunioideae; Petunia.
OX NCBI_TaxID=4102;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND REPRESSION BY ARBUSCULAR MYCORRHIZAL
RP FUNGI.
RC STRAIN=cv. W115, and cv. W138;
RX PubMed=18315538; DOI=10.1111/j.1365-313x.2008.03474.x;
RA Wegmueller S., Svistoonoff S., Reinhardt D., Stuurman J., Amrhein N.,
RA Bucher M.;
RT "A transgenic dTph1 insertional mutagenesis system for forward genetics in
RT mycorrhizal phosphate transport of Petunia.";
RL Plant J. 54:1115-1127(2008).
CC -!- FUNCTION: Low-affinity transporter for external inorganic phosphate
CC (Pi). {ECO:0000250|UniProtKB:Q8GSG4}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NH4(+)(in) = NH4(+)(out); Xref=Rhea:RHEA:28747,
CC ChEBI:CHEBI:28938; Evidence={ECO:0000250|UniProtKB:Q8GSG4};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q8GSG4};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- INDUCTION: Slightly repressed during arbuscular mycorrhiza (AM)
CC formation after inoculation with AM fungi (e.g. Glomus intraradices).
CC {ECO:0000269|PubMed:18315538}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; EU532761; ACB37439.1; -; Genomic_DNA.
DR AlphaFoldDB; B2CPI4; -.
DR SMR; B2CPI4; -.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR036259; MFS_trans_sf.
DR SUPFAM; SSF103473; SSF103473; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Phosphate transport; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN <1..>189
FT /note="Low affinity inorganic phosphate transporter 2"
FT /id="PRO_0000450037"
FT TOPO_DOM 1..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..111
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..165
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..189
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT NON_TER 1
FT /evidence="ECO:0000312|EMBL:ACB37439.1"
FT NON_TER 189
FT /evidence="ECO:0000312|EMBL:ACB37439.1"
SQ SEQUENCE 189 AA; 21656 MW; D4EBD0E346CD8E1F CRC64;
TARYTALVAK DAKRAAADMG KVLHVEIDPE DAKVERMAKD ESNQFGLFSW EFVRRHGLHL
FGTCSTWFLL DIAFYSQNLF QKDVFTAIGW IPPAKTMNAV QEVYKIARAQ TLIALCSTVP
GYWFTVAFID IIGRFAIQLM GFFFMTVFMF AIAIPYHHWT LQENRIGFVI MYSLTFFFAN
FGPNATTFV
