PHT13_ARATH
ID PHT13_ARATH Reviewed; 521 AA.
AC O48639; O04381;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Probable inorganic phosphate transporter 1-3;
DE Short=AtPht1;3;
DE AltName: Full=H(+)/Pi cotransporter;
GN Name=PHT1-3; Synonyms=PHT3, PT4; OrderedLocusNames=At5g43360;
GN ORFNames=MWF20.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Lu Y.-P., Zhen R.-G., Rea P.A.;
RT "AtPT4: a fourth member of the Arabidopsis phosphate transporter gene
RT family.";
RL (er) Plant Gene Register PGR97-082(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Mitsukawa N., Okumura S., Shibata D.;
RT "High-affinity phosphate transporter genes of Arabidopsis thaliana.";
RL Soil Sci. Plant Nutr. 43:971-974(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP INDUCTION, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12164813; DOI=10.1046/j.1365-313x.2002.01356.x;
RA Mudge S.R., Rae A.L., Diatloff E., Smith F.W.;
RT "Expression analysis suggests novel roles for members of the Pht1 family of
RT phosphate transporters in Arabidopsis.";
RL Plant J. 31:341-353(2002).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in roots, especially in the stele
CC of the primary root, the pericycle and trichoblasts of secondary roots.
CC To a lower extent, present in hydathodes and vascular tissues of young
CC leaves. {ECO:0000269|PubMed:12164813}.
CC -!- INDUCTION: In roots by phosphate starvation.
CC {ECO:0000269|PubMed:12164813}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; U97546; AAB69122.1; -; Genomic_DNA.
DR EMBL; AB000094; BAA24281.1; -; Genomic_DNA.
DR EMBL; AB025638; BAA97415.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94949.1; -; Genomic_DNA.
DR RefSeq; NP_199150.1; NM_123702.1.
DR AlphaFoldDB; O48639; -.
DR SMR; O48639; -.
DR STRING; 3702.AT5G43360.1; -.
DR PaxDb; O48639; -.
DR PRIDE; O48639; -.
DR ProteomicsDB; 236791; -.
DR EnsemblPlants; AT5G43360.1; AT5G43360.1; AT5G43360.
DR GeneID; 834354; -.
DR Gramene; AT5G43360.1; AT5G43360.1; AT5G43360.
DR KEGG; ath:AT5G43360; -.
DR Araport; AT5G43360; -.
DR TAIR; locus:2176471; AT5G43360.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; O48639; -.
DR OMA; NPRLNYG; -.
DR OrthoDB; 762280at2759; -.
DR PhylomeDB; O48639; -.
DR PRO; PR:O48639; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O48639; baseline and differential.
DR Genevisible; O48639; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004738; Phos_permease.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00887; 2A0109; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphate transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..521
FT /note="Probable inorganic phosphate transporter 1-3"
FT /id="PRO_0000050470"
FT TOPO_DOM 1..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..131
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..211
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..348
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..402
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 424..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..484
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 485..505
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 506..521
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 175
FT /note="G -> R (in Ref. 1; AAB69122)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 521 AA; 57257 MW; 5EB3FC6D42590E2F CRC64;
MADQQLGVLK ALDVAKTQLY HFTAIVIAGM GFFTDAYDLF CVSLVTKLLG RLYYFNPTSA
KPGSLPPHVA AAVNGVALCG TLAGQLFFGW LGDKLGRKKV YGITLIMMIL CSVASGLSLG
NSAKGVMTTL CFFRFWLGFG IGGDYPLSAT IMSEYANKKT RGAFIAAVFA MQGVGILAGG
FVALAVSSIF DKKFPSPTYE QDRFLSTPPQ ADYIWRIIVM FGALPAALTY YWRMKMPETA
RYTALVAKNI KQATADMSKV LQTDLELEER VEDDVKDPKK NYGLFSKEFL RRHGLHLLGT
TSTWFLLDIA FYSQNLFQKD IFSAIGWIPK AATMNAIHEV FKIARAQTLI ALCSTVPGYW
FTVAFIDIIG RFAIQLMGFF MMTVFMFAIA FPYNHWILPD NRIGFVVMYS LTFFFANFGP
NATTFIVPAE IFPARLRSTC HGISAATGKA GAIVGAFGFL YAAQPQDKTK TDAGYPPGIG
VKNSLIMLGV INFVGMLFTF LVPEPKGKSL EELSGEAEVD K
