PHT14_MEDTR
ID PHT14_MEDTR Reviewed; 528 AA.
AC Q8GSG4;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 102.
DE RecName: Full=Low affinity inorganic phosphate transporter 4 {ECO:0000303|PubMed:12368495};
DE Short=MtPT4 {ECO:0000303|PubMed:12368495};
DE Short=MtPht1;4 {ECO:0000305};
DE AltName: Full=Arbuscular mycorrhiza-induced phosphate transporter PT4 {ECO:0000305};
DE Short=AM-induced phosphate transporter PT4 {ECO:0000305};
DE AltName: Full=H(+)/Pi cotransporter PT4 {ECO:0000305};
GN Name=PT4 {ECO:0000303|PubMed:12368495};
GN OrderedLocusNames=MTR_1g028600 {ECO:0000312|EMBL:KEH40422.1};
GN ORFNames=MtrunA17_Chr1g0158991 {ECO:0000312|EMBL:RHN77834.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP SUBCELLULAR LOCATION, AND INDUCTION BY ARBUSCULAR MYCORRHIZAL FUNGI.
RC STRAIN=cv. Jemalong A17;
RX PubMed=12368495; DOI=10.1105/tpc.004861;
RA Harrison M.J., Dewbre G.R., Liu J.;
RT "A phosphate transporter from Medicago truncatula involved in the
RT acquisition of phosphate released by arbuscular mycorrhizal fungi.";
RL Plant Cell 14:2413-2429(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E.D., Geurts R., Cannon S.B.,
RA Udvardi M.K., Benedito V.A., Mayer K.F.X., Gouzy J., Schoof H.,
RA Van de Peer Y., Proost S., Cook D.R., Meyers B.C., Spannagl M., Cheung F.,
RA De Mita S., Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K.,
RA Murray J.D., Naoumkina M.A., Rosen B., Silverstein K.A.T., Tang H.,
RA Rombauts S., Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M.,
RA Bellec A., Berger A., Berges H., Bidwell S., Bisseling T., Choisne N.,
RA Couloux A., Denny R., Deshpande S., Dai X., Doyle J.J., Dudez A.-M.,
RA Farmer A.D., Fouteau S., Franken C., Gibelin C., Gish J., Goldstein S.,
RA Gonzalez A.J., Green P.J., Hallab A., Hartog M., Hua A., Humphray S.J.,
RA Jeong D.-H., Jing Y., Jocker A., Kenton S.M., Kim D.-J., Klee K., Lai H.,
RA Lang C., Lin S., Macmil S.L., Magdelenat G., Matthews L., McCorrison J.,
RA Monaghan E.L., Mun J.-H., Najar F.Z., Nicholson C., Noirot C.,
RA O'Bleness M., Paule C.R., Poulain J., Prion F., Qin B., Qu C., Retzel E.F.,
RA Riddle C., Sallet E., Samain S., Samson N., Sanders I., Saurat O.,
RA Scarpelli C., Schiex T., Segurens B., Severin A.J., Sherrier D.J., Shi R.,
RA Sims S., Singer S.R., Sinharoy S., Sterck L., Viollet A., Wang B.-B.,
RA Wang K., Wang M., Wang X., Warfsmann J., Weissenbach J., White D.D.,
RA White J.D., Wiley G.B., Wincker P., Xing Y., Yang L., Yao Z., Ying F.,
RA Zhai J., Zhou L., Zuber A., Denarie J., Dixon R.A., May G.D.,
RA Schwartz D.C., Rogers J., Quetier F., Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17;
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17;
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [5]
RP INDUCTION BY GLOMUS INTRARADICES.
RC STRAIN=cv. Jemalong J5;
RX PubMed=20687807; DOI=10.1094/mpmi-23-9-1175;
RA Paradi I., van Tuinen D., Morandi D., Ochatt S., Robert F., Jacas L.,
RA Dumas-Gaudot E.;
RT "Transcription of two blue copper-binding protein isogenes is highly
RT correlated with arbuscular mycorrhizal development in Medicago
RT truncatula.";
RL Mol. Plant Microbe Interact. 23:1175-1183(2010).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, CATALYTIC ACTIVITY, AND INDUCTION BY
RP ARBUSCULAR MYCORRHIZAL FUNGI.
RC STRAIN=cv. Jemalong A17;
RX PubMed=25841038; DOI=10.1105/tpc.114.131144;
RA Breuillin-Sessoms F., Floss D.S., Gomez S.K., Pumplin N., Ding Y.,
RA Levesque-Tremblay V., Noar R.D., Daniels D.A., Bravo A., Eaglesham J.B.,
RA Benedito V.A., Udvardi M.K., Harrison M.J.;
RT "Suppression of arbuscule degeneration in Medicago truncatula phosphate
RT transporter4 mutants is dependent on the ammonium transporter 2 family
RT protein AMT2;3.";
RL Plant Cell 27:1352-1366(2015).
RN [7]
RP INDUCTION BY RAM1 AND GLOMUS VERSIFORME.
RX PubMed=26511916; DOI=10.1104/pp.15.01155;
RA Park H.-J., Floss D.S., Levesque-Tremblay V., Bravo A., Harrison M.J.;
RT "Hyphal branching during arbuscule development requires reduced arbuscular
RT mycorrhiza1.";
RL Plant Physiol. 169:2774-2788(2015).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Jemalong A17;
RX PubMed=26476189; DOI=10.1111/pce.12659;
RA Volpe V., Giovannetti M., Sun X.-G., Fiorilli V., Bonfante P.;
RT "The phosphate transporters LjPT4 and MtPT4 mediate early root responses to
RT phosphate status in non mycorrhizal roots.";
RL Plant Cell Environ. 39:660-671(2016).
CC -!- FUNCTION: Low-affinity transporter for external inorganic phosphate
CC (Pi) probably involved in the acquisition of phosphate released by
CC arbuscular mycorrhizal (AM) fungi (e.g. Gigaspora gigantea, Glomus
CC versiforme and G.intraradices) during AM symbiosis; required for
CC propper mycorrhizal arbuscule morphology (PubMed:12368495,
CC PubMed:25841038). Acts as a Pi-sensing machinery at the root tip level,
CC independently of AM fungi, involved in the regulation of early root
CC branching and lateral roots formation (PubMed:26476189).
CC {ECO:0000269|PubMed:12368495, ECO:0000269|PubMed:25841038,
CC ECO:0000269|PubMed:26476189}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NH4(+)(in) = NH4(+)(out); Xref=Rhea:RHEA:28747,
CC ChEBI:CHEBI:28938; Evidence={ECO:0000269|PubMed:12368495,
CC ECO:0000269|PubMed:25841038};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=500 uM for inorganic phosphate {ECO:0000305|PubMed:12368495};
CC pH dependence:
CC Optimum pH is 3-4. {ECO:0000269|PubMed:12368495};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12368495};
CC Multi-pass membrane protein {ECO:0000255}. Note=Present on the
CC periarbuscular membrane in cells containing arbuscules during
CC arbuscular mycorrhizal (AM) symbiosis with AM fungi (e.g. Gigaspora
CC gigantea, Glomus versiforme and G.intraradices).
CC {ECO:0000269|PubMed:12368495}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in mycorrhizal roots
CC (PubMed:12368495). Also observed in root tips of non-mycorrhizal roots,
CC in a phosphate (Pi) depended-manner, highest expression levels being
CC observed in low Pi conditions (PubMed:26476189).
CC {ECO:0000269|PubMed:12368495, ECO:0000269|PubMed:26476189}.
CC -!- DEVELOPMENTAL STAGE: Expressed in cortical cells containing arbuscules
CC of mycorrhizal roots upon arbuscular mycorrhizal (AM) symbiosis with AM
CC fungi (e.g. Gigaspora gigantea, Glomus versiforme and G.intraradices).
CC {ECO:0000269|PubMed:25841038}.
CC -!- INDUCTION: Accumulates in roots, in a RAM1-dependent manner, during
CC colonization by arbuscular mycorrhizal (AM) fungi (e.g. Gigaspora
CC gigantea, Glomus versiforme and G.intraradices) (PubMed:26511916,
CC PubMed:20687807, PubMed:25841038). Induced in root tips by low
CC phosphate (Pi) levels (PubMed:26476189). {ECO:0000269|PubMed:20687807,
CC ECO:0000269|PubMed:25841038, ECO:0000269|PubMed:26476189,
CC ECO:0000269|PubMed:26511916}.
CC -!- DISRUPTION PHENOTYPE: Impaired phosphate (Pi) starvation induction of
CC lateral roots formation (PubMed:26476189). Plants missing PT4 or both
CC PT4 and PT8 fail to establish arbuscular mycorrhizal (AM) symbiosis
CC with AM fungi in high nitrogen conditions, leading to premature
CC arbuscule degeneration (PAD); these phenotypes are suppressed in
CC nitrogen-deprived conditions in an AMT2-3-dependent manner
CC (PubMed:25841038). {ECO:0000269|PubMed:25841038,
CC ECO:0000269|PubMed:26476189}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; AY116210; AAM76743.1; -; mRNA.
DR EMBL; AY116211; AAM76744.1; -; Genomic_DNA.
DR EMBL; CM001217; KEH40422.1; -; Genomic_DNA.
DR EMBL; PSQE01000001; RHN77834.1; -; Genomic_DNA.
DR RefSeq; XP_013466381.1; XM_013610927.1.
DR AlphaFoldDB; Q8GSG4; -.
DR SMR; Q8GSG4; -.
DR EnsemblPlants; KEH40422; KEH40422; MTR_1g028600.
DR GeneID; 25482376; -.
DR Gramene; KEH40422; KEH40422; MTR_1g028600.
DR KEGG; mtr:MTR_1g028600; -.
DR HOGENOM; CLU_001265_46_14_1; -.
DR OrthoDB; 762280at2759; -.
DR Proteomes; UP000002051; Chromosome 1.
DR Proteomes; UP000265566; Chromosome 1.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0085042; C:periarbuscular membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0036377; P:arbuscular mycorrhizal association; IMP:UniProtKB.
DR GO; GO:0043562; P:cellular response to nitrogen levels; IMP:UniProtKB.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IMP:UniProtKB.
DR GO; GO:0010247; P:detection of phosphate ion; IMP:UniProtKB.
DR GO; GO:0010311; P:lateral root formation; IMP:UniProtKB.
DR GO; GO:0006817; P:phosphate ion transport; IDA:UniProtKB.
DR GO; GO:0009610; P:response to symbiotic fungus; IEP:UniProtKB.
DR GO; GO:0055085; P:transmembrane transport; IDA:UniProtKB.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004738; Phos_permease.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00887; 2A0109; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphate transport; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..528
FT /note="Low affinity inorganic phosphate transporter 4"
FT /id="PRO_0000450032"
FT TOPO_DOM 1..18
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 19..39
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 40..68
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 69..89
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 90..96
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 97..117
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..122
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 123..143
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 144..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 159..179
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..208
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 209..229
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 230..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 293..313
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..341
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 342..362
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 372..392
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..401
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 402..422
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 423..433
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 434..454
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 455..468
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 469..489
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 490..528
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REGION 497..528
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 528 AA; 58923 MW; 24BB4496FC673FC0 CRC64;
MGLEVLEALD SARTQWYHVT AIVIAGMGFF TDAYDLFCIS TVSKLLGRLY YFDPSTNKPG
KLPPSVNNVV TGVALVGTLS GQLVFGWLGD KLGRKKVYGV TLIIMVACAI CSGLSFGSSA
KSVMITLCFF RFWLGFGIGG DYPLSATIMS EYANKRTRGA FIAAVFAMQG VGIIFAGLVS
MVFSGIFKAY YQAPRFNEDP ILSTQPEGDL LWRLILMIGA VPAAMTYYWR MKMPETGRYT
AIVEGNAKQA AADMARVLDI EIIAEQDKLA EFKAANDYPL WSSEFFNRHG RHLIGTMSCW
FLLDIAFYSQ NLTQKDIYPA MGLIRQDKEM NAIDEVFQTS RAMFVVALFG TFPGYWFTVF
FIEKLGRFKI QLVGFFMMSF FMFVIGVKYE YLKDENKNLF ALLYGLTFFF ANFGPNSTTF
VLPAELFPTR VRSTCHAFSA ASGKAGAMVG AFGIQYYTLD GTPRKIRRAM MILAFTNLIG
FFCTFLVTET KGRSLEEISG EDGRESELTA TPNDRAPGIR QDSRTEKM
