PHT15_ARATH
ID PHT15_ARATH Reviewed; 542 AA.
AC Q8GYF4; O50040; Q8S9I0;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Probable inorganic phosphate transporter 1-5;
DE Short=AtPht1;5;
DE AltName: Full=H(+)/Pi cotransporter;
GN Name=PHT1-5; Synonyms=PHT5; OrderedLocusNames=At2g32830; ORFNames=F24L7.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872450; DOI=10.1093/dnares/5.5.261;
RA Okumura S., Mitsukawa N., Shirano Y., Shibata D.;
RT "Phosphate transporter gene family of Arabidopsis thaliana.";
RL DNA Res. 5:261-269(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 274-542.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [6]
RP TISSUE SPECIFICITY, INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12164813; DOI=10.1046/j.1365-313x.2002.01356.x;
RA Mudge S.R., Rae A.L., Diatloff E., Smith F.W.;
RT "Expression analysis suggests novel roles for members of the Pht1 family of
RT phosphate transporters in Arabidopsis.";
RL Plant J. 31:341-353(2002).
RN [7]
RP INDUCTION.
RX PubMed=15604713; DOI=10.1007/s11103-004-1965-5;
RA Misson J., Thibaud M.-C., Bechtold N., Raghothama K., Nussaume L.;
RT "Transcriptional regulation and functional properties of Arabidopsis
RT Pht1;4, a high affinity transporter contributing greatly to phosphate
RT uptake in phosphate deprived plants.";
RL Plant Mol. Biol. 55:727-741(2004).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Mainly expressed in shoots, First throughout
CC cotyledons and hypocotyls and later restricted to vascular tissues,
CC mostly in phloem. Present in senescing leaves. Also expressed in floral
CC buds and sepals. {ECO:0000269|PubMed:12164813}.
CC -!- INDUCTION: Slightly induced in roots during phosphate starvation.
CC {ECO:0000269|PubMed:12164813, ECO:0000269|PubMed:15604713}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL77700.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAC42323.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB000093; BAA24280.1; -; Genomic_DNA.
DR EMBL; AC003033; AAB91982.1; -; Genomic_DNA.
DR EMBL; AC003974; AAM14902.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08747.1; -; Genomic_DNA.
DR EMBL; AY075694; AAL77700.1; ALT_FRAME; mRNA.
DR EMBL; AK117670; BAC42323.1; ALT_INIT; mRNA.
DR PIR; T01124; T01124.
DR RefSeq; NP_180842.1; NM_128843.4.
DR AlphaFoldDB; Q8GYF4; -.
DR SMR; Q8GYF4; -.
DR STRING; 3702.AT2G32830.1; -.
DR PaxDb; Q8GYF4; -.
DR PRIDE; Q8GYF4; -.
DR ProteomicsDB; 234722; -.
DR EnsemblPlants; AT2G32830.1; AT2G32830.1; AT2G32830.
DR GeneID; 817844; -.
DR Gramene; AT2G32830.1; AT2G32830.1; AT2G32830.
DR KEGG; ath:AT2G32830; -.
DR Araport; AT2G32830; -.
DR TAIR; locus:2046432; AT2G32830.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; Q8GYF4; -.
DR OMA; FFEIGPN; -.
DR OrthoDB; 762280at2759; -.
DR PhylomeDB; Q8GYF4; -.
DR PRO; PR:Q8GYF4; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8GYF4; baseline and differential.
DR Genevisible; Q8GYF4; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; ISS:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006817; P:phosphate ion transport; IMP:TAIR.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004738; Phos_permease.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00887; 2A0109; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphate transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..542
FT /note="Probable inorganic phosphate transporter 1-5"
FT /id="PRO_0000050472"
FT TOPO_DOM 1..24
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 46..70
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 92..99
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 121..131
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..161
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 183..211
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..294
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 316..350
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..373
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 395..404
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 426..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 444..464
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 465..485
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 486..506
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 507..542
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 542 AA; 59214 MW; 9B63C154899A8AC1 CRC64;
MAKKGKEVLN ALDAAKTQMY HFTAIVIAGM GFFTDAYDLF SISLVTKLLG RIYYHVDSSK
KPGTLPPNVA AAVNGVAFCG TLAGQLFFGW LGDKLGRKKV YGITLMLMVL CSLGSGLSFG
HSANGVMATL CFFRFWLGFG IGGDYPLSAT IMSEYANKKT RGAFIAAVFA MQGFGILAGG
IVSLIVSSTF DHAFKAPTYE VDPVGSTVPQ ADYVWRIVLM FGAIPALLTY YWRMKMPETA
RYTALVARNT KQAASDMSKV LQVDLIAEEE AQSNSNSSNP NFTFGLFTRE FARRHGLHLL
GTTTTWFLLD IAYYSSNLFQ KDIYTAIGWI PAAETMNAIH EVFTVSKAQT LIALCGTVPG
YWFTVAFIDI LGRFFIQLMG FIFMTIFMFA LAIPYDHWRH RENRIGFLIM YSLTMFFANF
GPNATTFVVP AEIFPARLRS TCHGISAASG KAGAIVGAFG FLYAAQSSDS EKTDAGYPPG
IGVRNSLLML ACVNFLGIVF TLLVPESKGK SLEEISREDE EQSGGDTVVE MTVANSGRKV
PV
