PHT16_ORYSJ
ID PHT16_ORYSJ Reviewed; 534 AA.
AC Q8H6H0; A0A0P0XIB8;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Inorganic phosphate transporter 1-6;
DE Short=OsPT6;
DE Short=OsPht1;6;
DE AltName: Full=H(+)/Pi cotransporter;
DE AltName: Full=OsLPT1;
DE AltName: Full=OsLPT6:1;
GN Name=PHT1-6; Synonyms=PT6; OrderedLocusNames=Os08g0564000, LOC_Os08g45000;
GN ORFNames=P0543D10.41, P0705A05.110;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RX PubMed=12271140; DOI=10.1073/pnas.202474599;
RA Paszkowski U., Kroken S., Roux C., Briggs S.P.;
RT "Rice phosphate transporters include an evolutionarily divergent gene
RT specifically activated in arbuscular mycorrhizal symbiosis.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13324-13329(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP INDUCTION.
RX PubMed=18563580; DOI=10.1007/s10529-008-9757-7;
RA Seo H.-M., Jung Y., Song S., Kim Y., Kwon T., Kim D.-H., Jeung S.-J.,
RA Yi Y.-B., Yi G., Nam M.-H., Nam J.;
RT "Increased expression of OsPT1, a high-affinity phosphate transporter,
RT enhances phosphate acquisition in rice.";
RL Biotechnol. Lett. 30:1833-1838(2008).
RN [7]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=18980647; DOI=10.1111/j.1365-313x.2008.03726.x;
RA Ai P., Sun S., Zhao J., Fan X., Xin W., Guo Q., Yu L., Shen Q., Wu P.,
RA Miller A.J., Xu G.;
RT "Two rice phosphate transporters, OsPht1;2 and OsPht1;6, have different
RT functions and kinetic properties in uptake and translocation.";
RL Plant J. 57:798-809(2009).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate
CC (Pi). Probably involved in Pi uptake, translocation and internal
CC transport throughout the plant. {ECO:0000269|PubMed:18980647}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=97 uM for inorganic phosphate (Pi) {ECO:0000269|PubMed:18980647};
CC Note=Measured in yeast cells deficient in Pi uptake.;
CC pH dependence:
CC Optimum pH is 6.0. {ECO:0000269|PubMed:18980647};
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Highly expressed in leaves and at low levels in
CC roots. Expressed in leaf xylem parenchyma cells.
CC {ECO:0000269|PubMed:18980647}.
CC -!- INDUCTION: In roots and leaves by phosphate starvation. Down-regulated
CC in roots when colonized by the mycorrhizal fungus G. intraradices.
CC Down-regulated by high phosphate in suspension cell culture.
CC {ECO:0000269|PubMed:12271140, ECO:0000269|PubMed:18563580,
CC ECO:0000269|PubMed:18980647}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; AF536966; AAN39047.1; -; Genomic_DNA.
DR EMBL; AP004587; BAD09632.1; -; Genomic_DNA.
DR EMBL; AP004623; BAC55993.1; -; Genomic_DNA.
DR EMBL; AP008214; BAF24441.1; -; Genomic_DNA.
DR EMBL; AP014964; BAT06733.1; -; Genomic_DNA.
DR EMBL; AK119787; BAG99790.1; -; mRNA.
DR RefSeq; XP_015649112.1; XM_015793626.1.
DR AlphaFoldDB; Q8H6H0; -.
DR SMR; Q8H6H0; -.
DR STRING; 4530.OS08T0564000-01; -.
DR TCDB; 2.A.1.9.5; the major facilitator superfamily (mfs).
DR PaxDb; Q8H6H0; -.
DR PRIDE; Q8H6H0; -.
DR EnsemblPlants; Os08t0564000-01; Os08t0564000-01; Os08g0564000.
DR GeneID; 4346342; -.
DR Gramene; Os08t0564000-01; Os08t0564000-01; Os08g0564000.
DR KEGG; osa:4346342; -.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; Q8H6H0; -.
DR OMA; YKNAQQH; -.
DR OrthoDB; 762280at2759; -.
DR Proteomes; UP000000763; Chromosome 8.
DR Proteomes; UP000059680; Chromosome 8.
DR Genevisible; Q8H6H0; OS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004738; Phos_permease.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00887; 2A0109; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Membrane; Phosphate transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..534
FT /note="Inorganic phosphate transporter 1-6"
FT /id="PRO_0000365486"
FT TOPO_DOM 1..29
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 30..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 51..75
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 76..96
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 97..104
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..127
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..168
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 169..189
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 190..216
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..294
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 316..350
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 351..371
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..375
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..405
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 427..445
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 446..466
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 467..486
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 487..507
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 508..534
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 514..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 534 AA; 57786 MW; 46C9028EEBACEB40 CRC64;
MGGGGGEQQQ LEVLHALDVA KTQWYHFTAI VVAGMGFFTD AYDLFCISLV TKLLGRIYYR
VDGSPSPGTL PPHVSASVNG VAFVGTLSGQ LFFGWLGDKL GRKRVYGITL MLMVLCSLAS
ALSFGHTPTS VMATLCFFRF WLGFGIGGDY PLSATIMSEY ANKKTRGAFI AAVFAMQGFG
IITGGLVAIL VSASFRAAFP APPYGEDPVA STPPQADFVW RIILMLGALP AALTYYWRTK
MPETARYTAL VANNAKQAAA DMSKVLQVVE MRNIGNNGGS RRPFGLFSGE FVRRHGLHLV
GTSATWLLLD IAFYSQNLFQ KDIFSAVGWI PKAATMSALE ELFRIARAQT LIALCGTVPG
YWFTVALIDV VGRFKIQAVG FFMMTLFMLT LALPYHHWTA PGKNHVGFLL LYGLTFFFAN
FGPNSTTFIV PAEIFPARLR ATCHGISAAS GKLGAIVGSF GFLYLAQSPD RSKTEHGYPP
GIGVRNSLFL LAACNLLGLL FTFLVPESKG KSLEEMSGDA EAQEEAPPPL QTVL
