PHT18_ARATH
ID PHT18_ARATH Reviewed; 534 AA.
AC Q9SYQ1; Q1PFU6;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 2.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Probable inorganic phosphate transporter 1-8;
DE Short=AtPht1;8;
DE AltName: Full=H(+)/Pi cotransporter;
GN Name=PHT1-8; OrderedLocusNames=At1g20860; ORFNames=F9H16.16;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12164813; DOI=10.1046/j.1365-313x.2002.01356.x;
RA Mudge S.R., Rae A.L., Diatloff E., Smith F.W.;
RT "Expression analysis suggests novel roles for members of the Pht1 family of
RT phosphate transporters in Arabidopsis.";
RL Plant J. 31:341-353(2002).
RN [5]
RP INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=15604713; DOI=10.1007/s11103-004-1965-5;
RA Misson J., Thibaud M.-C., Bechtold N., Raghothama K., Nussaume L.;
RT "Transcriptional regulation and functional properties of Arabidopsis
RT Pht1;4, a high affinity transporter contributing greatly to phosphate
RT uptake in phosphate deprived plants.";
RL Plant Mol. Biol. 55:727-741(2004).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: In roots. {ECO:0000269|PubMed:15604713}.
CC -!- INDUCTION: Slightly induced in roots during phosphate starvation.
CC {ECO:0000269|PubMed:12164813, ECO:0000269|PubMed:15604713}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD30606.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007369; AAD30606.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30032.1; -; Genomic_DNA.
DR EMBL; DQ446267; ABE65636.1; -; mRNA.
DR PIR; B86341; B86341.
DR RefSeq; NP_173510.1; NM_101939.1.
DR AlphaFoldDB; Q9SYQ1; -.
DR SMR; Q9SYQ1; -.
DR STRING; 3702.AT1G20860.1; -.
DR PaxDb; Q9SYQ1; -.
DR EnsemblPlants; AT1G20860.1; AT1G20860.1; AT1G20860.
DR GeneID; 838678; -.
DR Gramene; AT1G20860.1; AT1G20860.1; AT1G20860.
DR KEGG; ath:AT1G20860; -.
DR Araport; AT1G20860; -.
DR TAIR; locus:2037390; AT1G20860.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; Q9SYQ1; -.
DR PhylomeDB; Q9SYQ1; -.
DR PRO; PR:Q9SYQ1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SYQ1; baseline and differential.
DR Genevisible; Q9SYQ1; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphate transport; Phosphoprotein; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..534
FT /note="Probable inorganic phosphate transporter 1-8"
FT /id="PRO_0000050475"
FT TOPO_DOM 1..21
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 22..42
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 43..61
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..90
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..153
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 175..199
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 221..281
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..333
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 334..354
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 355..361
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 383..396
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 397..417
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 418..431
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 432..452
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 453..472
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 473..493
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 494..534
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 501..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..534
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 514
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96303"
SQ SEQUENCE 534 AA; 59163 MW; 56499F0F52676DBB CRC64;
MPIKVLSSLD VARTQWYHFK AIIVAGMGLF TDAYDLFCIA PVMKMISHVY YNGDSINTAV
LSTSYAIALL GTATGQLVFG YLGDRVGRRR VYGLCLIIMI LSSFGCGFSV CTTRRSCVMV
SLGFFRFFLG LGIGGDYPLS ATIMSEFANK RTRGAFIAAV FSMQGLGILV SSAVTMAVCV
AFKRSGGGLE VDAAAPTEAD LAWRLILMIG ALPAALTFYW RMLMPETARY TALVENNIVQ
AAKDMQRVMS RSHISDEATT DPPPPPPPPS YKLFSRCFFR LHGRDLFAAS FNWFLVDIVF
YTSNLLLSHI FSHYSKKPST AENVYDAAFE VAELGAIIAA CSTIPGYWFT VYFIDKIGRV
KIQIMGFFFM AVIYLVAGIP YSWYWSKHEH NNKGFMVLYG LVFFFCNFGP NTTTFIIPAE
HFPARFRSTC HGISGAAGKL GAIVGTVGFL WATKKMESDD KNQIYPEVNR MRIAFLILGG
VCIAGILVTY FFTKETMGRS LEENEHDQDN NAESEDEPQI VDGQSSVSTL LQTR
