PHT19_ARATH
ID PHT19_ARATH Reviewed; 532 AA.
AC Q9S735;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Probable inorganic phosphate transporter 1-9;
DE Short=AtPht1;9;
DE AltName: Full=H(+)/Pi cotransporter;
GN Name=PHT1-9; OrderedLocusNames=At1g76430; ORFNames=F14G6.3, F15M4.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP INDUCTION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12164813; DOI=10.1046/j.1365-313x.2002.01356.x;
RA Mudge S.R., Rae A.L., Diatloff E., Smith F.W.;
RT "Expression analysis suggests novel roles for members of the Pht1 family of
RT phosphate transporters in Arabidopsis.";
RL Plant J. 31:341-353(2002).
RN [5]
RP INDUCTION.
RX PubMed=15604713; DOI=10.1007/s11103-004-1965-5;
RA Misson J., Thibaud M.-C., Bechtold N., Raghothama K., Nussaume L.;
RT "Transcriptional regulation and functional properties of Arabidopsis
RT Pht1;4, a high affinity transporter contributing greatly to phosphate
RT uptake in phosphate deprived plants.";
RL Plant Mol. Biol. 55:727-741(2004).
CC -!- FUNCTION: High-affinity transporter for external inorganic phosphate.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- INDUCTION: Slightly induced in roots during phosphate starvation.
CC {ECO:0000269|PubMed:12164813, ECO:0000269|PubMed:15604713}.
CC -!- MISCELLANEOUS: Although related to the sugar transporter family, it
CC does not transport sugars.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC phosphate:H(+) symporter (TC 2.A.1.9) family. {ECO:0000305}.
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DR EMBL; AC012394; AAF16658.1; -; Genomic_DNA.
DR EMBL; AC015450; AAG51941.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35840.1; -; Genomic_DNA.
DR EMBL; BT008324; AAP37683.1; -; mRNA.
DR PIR; A96792; A96792.
DR RefSeq; NP_177769.1; NM_106293.4.
DR AlphaFoldDB; Q9S735; -.
DR SMR; Q9S735; -.
DR BioGRID; 29195; 2.
DR IntAct; Q9S735; 2.
DR STRING; 3702.AT1G76430.1; -.
DR PaxDb; Q9S735; -.
DR PRIDE; Q9S735; -.
DR ProteomicsDB; 236158; -.
DR EnsemblPlants; AT1G76430.1; AT1G76430.1; AT1G76430.
DR GeneID; 843976; -.
DR Gramene; AT1G76430.1; AT1G76430.1; AT1G76430.
DR KEGG; ath:AT1G76430; -.
DR Araport; AT1G76430; -.
DR TAIR; locus:2011681; AT1G76430.
DR eggNOG; KOG0252; Eukaryota.
DR HOGENOM; CLU_001265_46_14_1; -.
DR InParanoid; Q9S735; -.
DR OMA; YAWLIWW; -.
DR OrthoDB; 762280at2759; -.
DR PhylomeDB; Q9S735; -.
DR PRO; PR:Q9S735; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9S735; baseline and differential.
DR Genevisible; Q9S735; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015415; F:ATPase-coupled phosphate ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015114; F:phosphate ion transmembrane transporter activity; IGI:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:1901684; P:arsenate ion transmembrane transport; IMP:TAIR.
DR GO; GO:0006817; P:phosphate ion transport; IMP:TAIR.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00217; SUGAR_TRANSPORT_2; 1.
PE 2: Evidence at transcript level;
KW Membrane; Phosphate transport; Reference proteome; Symport; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..532
FT /note="Probable inorganic phosphate transporter 1-9"
FT /id="PRO_0000050476"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 44..62
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..91
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 113..124
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 125..145
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..154
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 155..175
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 176..207
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..343
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..371
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 372..392
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 393..406
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 407..427
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 428..441
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 442..462
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 463..478
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 479..499
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 500..532
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 509..532
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 513..532
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 532 AA; 58701 MW; DAF35045A6960DDD CRC64;
MPELSLLSAL DAARIQWYHF KAIIVAGMGL FTDAYDLFCI APIMKMISQI YYHKDSIGTA
LLSTSYAIAL LGTALGQLIF GYLGDRVGRR KVYGLSLLIM VFSSFGCGFS VCTTRRSCVM
VSLGFFRFVL GLGIGGDYPL SATIMSEFAN KRTRGAFIAA VFSMQGLGIL MSSAVTMVVC
LAFKNAGEGS SEKTNVAGLE TLAPPESDIA WRLILMIGAL PAALTFYWRM LMPETARYTA
LVENNVVQAA KDMQRVMSVS MISQITEDSS SELEQPPSSS SYKLFSRRFL SLHGRDLFAA
SANWFLVDVV FYTSNLLLSQ IFNFSNKPLN STNVYDSAFE VAKLAAIVAA CSTIPGYWFT
VYFIDKIGRV KIQMMGFFLM AVVYLVAGIP YSWYWSKHEK TNKGFMVLYG LIFFFSNFGP
NTTTFIIPAE LFPARFRSTC HGISGAAGKF GAIVGTVGFL WATRHHEEDG FPDVKRVRIA
FLILGGVCIA GMIVTYLFTR ETMGRSLEEN EDEIVSTSAG SSPANELLRR QY
