ID   PHT1_ORYSJ              Reviewed;         445 AA.
AC   Q5SMM8;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Putrescine hydroxycinnamoyltransferase 1 {ECO:0000305};
DE            Short=OsPHT1 {ECO:0000303|PubMed:27354554};
DE            EC=2.3.1.- {ECO:0000305};
DE   AltName: Full=BAHD-like hydroxycinnamoyl transferase HCT3 {ECO:0000305};
DE   AltName: Full=Hydroxycinnamoyltransferase 3 {ECO:0000305};
DE            Short=OsHCT3 {ECO:0000303|PubMed:22285622};
GN   Name=PHT1 {ECO:0000303|PubMed:27354554};
GN   Synonyms=HCT3 {ECO:0000303|PubMed:22285622};
GN   OrderedLocusNames=Os06g0184900 {ECO:0000312|EMBL:BAF18912.1},
GN   LOC_Os06g08580 {ECO:0000305};
GN   ORFNames=P0554A06.23 {ECO:0000312|EMBL:BAD72525.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   TISSUE SPECIFICITY.
RX   PubMed=22285622; DOI=10.1016/j.phytochem.2011.12.015;
RA   Kim I.A., Kim B.G., Kim M., Ahn J.H.;
RT   "Characterization of hydroxycinnamoyltransferase from rice and its
RT   application for biological synthesis of hydroxycinnamoyl glycerols.";
RL   Phytochemistry 76:25-31(2012).
RN   [6]
RP   FUNCTION.
RX   PubMed=27354554; DOI=10.1105/tpc.16.00265;
RA   Peng M., Gao Y., Chen W., Wang W., Shen S., Shi J., Wang C., Zhang Y.,
RA   Zou L., Wang S., Wan J., Liu X., Gong L., Luo J.;
RT   "Evolutionarily distinct BAHD N-acyltransferases are responsible for
RT   natural variation of aromatic amine conjugates in rice.";
RL   Plant Cell 28:1533-1550(2016).
CC   -!- FUNCTION: Hydroxycinnamoyl transferase that catalyzes the transfer of
CC       an acyl from p-coumaryol-CoA to putrescine, to produce coumaroyl
CC       putrescine. {ECO:0000269|PubMed:27354554}.
CC   -!- TISSUE SPECIFICITY: Expressed in leaves. {ECO:0000269|PubMed:22285622}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AP005919; BAD72525.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF18912.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS96506.1; -; Genomic_DNA.
DR   EMBL; AK109842; BAG98925.1; -; mRNA.
DR   RefSeq; XP_015643300.1; XM_015787814.1.
DR   AlphaFoldDB; Q5SMM8; -.
DR   SMR; Q5SMM8; -.
DR   STRING; 4530.OS06T0184900-01; -.
DR   PaxDb; Q5SMM8; -.
DR   PRIDE; Q5SMM8; -.
DR   EnsemblPlants; Os06t0184900-01; Os06t0184900-01; Os06g0184900.
DR   GeneID; 4340333; -.
DR   Gramene; Os06t0184900-01; Os06t0184900-01; Os06g0184900.
DR   KEGG; osa:4340333; -.
DR   eggNOG; ENOG502QTJX; Eukaryota.
DR   HOGENOM; CLU_014546_2_0_1; -.
DR   InParanoid; Q5SMM8; -.
DR   OMA; NWISTYD; -.
DR   OrthoDB; 1130893at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; Q5SMM8; differential.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR   GO; GO:0050734; F:hydroxycinnamoyltransferase activity; IDA:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..445
FT                   /note="Putrescine hydroxycinnamoyltransferase 1"
FT                   /id="PRO_0000437756"
FT   ACT_SITE        154
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT   ACT_SITE        388
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8W1W9"
SQ   SEQUENCE   445 AA;  47201 MW;  F8016DD68E93A7CD CRC64;
     MAVEIVKSSM VTAGEATPEH RIWLSNLDLL VARSHTPTVY VYRRTGPDSD AAFFSPDVLK
     AALSKVLVPF YPLAGRLAQD SAGRPEISCT GEGVLFVTAR SGATIDDLGD LAPSDELRRM
     LVPAADVAAA SILAMFQVTF FRCGGVCLGA AIHHTAADGL AALDFVNTWA AIARDVAGDG
     EAAAAAVQRP WLDRTLLRAR SPPAVRFDHA EYSRRRGGGS KLPFDSAILP MSKNQLNALK
     GAGAGAGKRL STFTAVVAHV WRCACKARGL AVAGTEAATR LYMTADARTR LHPPLPRGYL
     GNAIFRASAV SKVSDIVAAG PLGAVAEKVS AATARLDDGY VRSLLDHLEQ TAAAASGGAA
     GLRKGEWVMP ESDLWVISWQ GLPLYDADFG WGRPAFMGRA CLQFSGLVYL VPGRDDGDGR
     LDVVVAMDPE SLAKFKDVFY EELKC