PHT21_ARATH
ID PHT21_ARATH Reviewed; 587 AA.
AC Q38954; Q3EAZ6;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Inorganic phosphate transporter 2-1, chloroplastic;
DE AltName: Full=H(+)/Pi cotransporter;
DE Short=AtPht2;1;
DE Flags: Precursor;
GN Name=PHT2-1; Synonyms=PHT2; OrderedLocusNames=At3g26570; ORFNames=MFE16.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=8932388; DOI=10.1093/nar/24.21.4313;
RA Quigley F., Dao P., Cottet A., Mache R.;
RT "Sequence analysis of an 81 kb contig from Arabidopsis thaliana chromosome
RT III.";
RL Nucleic Acids Res. 24:4313-4318(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION, AND
RP BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=cv. Columbia;
RX PubMed=10559441; DOI=10.2307/3871016;
RA Daram P., Brunner S., Rausch C., Steiner C., Amrhein N., Bucher M.;
RT "Pht2;1 encodes a low-affinity phosphate transporter from Arabidopsis.";
RL Plant Cell 11:2153-2166(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RC STRAIN=cv. Columbia;
RX PubMed=12172020; DOI=10.1105/tpc.002220;
RA Versaw W.K., Harrison M.J.;
RT "A chloroplast phosphate transporter, PHT2;1, influences allocation of
RT phosphate within the plant and phosphate-starvation responses.";
RL Plant Cell 14:1751-1766(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP TISSUE SPECIFICITY, INDUCTION, DEVELOPMENTAL STAGE, AND SUBCELLULAR
RP LOCATION.
RX PubMed=15200639; DOI=10.1111/j.1365-313x.2004.02106.x;
RA Rausch C., Zimmermann P., Amrhein N., Bucher M.;
RT "Expression analysis suggests novel roles for the plastidic phosphate
RT transporter Pht2;1 in auto- and heterotrophic tissues in potato and
RT Arabidopsis.";
RL Plant J. 39:13-28(2004).
CC -!- FUNCTION: Low affinity H(+)/Pi chloroplastic cotransporter. Involved in
CC inorganic phosphate (orthophosphate, Pi) uptake in green parts of
CC plants in Pi-sufficient conditions. Required for Pi retranslocation
CC during Pi deprivation. {ECO:0000269|PubMed:12172020}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=0.4 mM for Pi (at pH 4 and 30 degrees Celsius)
CC {ECO:0000269|PubMed:10559441};
CC Note=Highly specific for Pi among other anions.;
CC pH dependence:
CC Optimum pH is 4. {ECO:0000269|PubMed:10559441};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000305|PubMed:12172020, ECO:0000305|PubMed:15200639}; Multi-pass
CC membrane protein {ECO:0000305|PubMed:12172020,
CC ECO:0000305|PubMed:15200639}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q38954-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q38954-2; Sequence=VSP_018146;
CC -!- TISSUE SPECIFICITY: Mostly expressed in young green tissues. Present in
CC both auto- and heterotrophic tissues. Also expressed in root stele.
CC {ECO:0000269|PubMed:10559441, ECO:0000269|PubMed:15200639}.
CC -!- DEVELOPMENTAL STAGE: During flower development, expressed in sepals,
CC stamens and siliques. In seeds, confined to the connecting site of the
CC funiculus. In seedlings, expressed in cotyledons and hypocotyls.
CC Restricted progressively to vascular tissues as plants become older.
CC {ECO:0000269|PubMed:15200639}.
CC -!- INDUCTION: Induction by light is inhibited by abscisic acid (ABA) and
CC cycloheximide. {ECO:0000269|PubMed:10559441,
CC ECO:0000269|PubMed:12172020, ECO:0000269|PubMed:15200639}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the inorganic phosphate transporter (PiT) (TC
CC 2.A.20.2) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X97484; CAA66116.1; -; mRNA.
DR EMBL; X98130; CAA66826.1; -; Genomic_DNA.
DR EMBL; AJ302645; CAC15560.1; -; mRNA.
DR EMBL; AF515591; AAM53960.1; -; mRNA.
DR EMBL; AB028611; BAB01839.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77180.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77181.1; -; Genomic_DNA.
DR RefSeq; NP_189289.2; NM_113565.3. [Q38954-2]
DR RefSeq; NP_850633.1; NM_180302.2. [Q38954-1]
DR AlphaFoldDB; Q38954; -.
DR SMR; Q38954; -.
DR STRING; 3702.AT3G26570.1; -.
DR TCDB; 2.A.20.2.4; the inorganic phosphate transporter (pit) family.
DR PaxDb; Q38954; -.
DR PRIDE; Q38954; -.
DR ProteomicsDB; 235110; -. [Q38954-1]
DR EnsemblPlants; AT3G26570.1; AT3G26570.1; AT3G26570. [Q38954-2]
DR EnsemblPlants; AT3G26570.2; AT3G26570.2; AT3G26570. [Q38954-1]
DR GeneID; 822265; -.
DR Gramene; AT3G26570.1; AT3G26570.1; AT3G26570. [Q38954-2]
DR Gramene; AT3G26570.2; AT3G26570.2; AT3G26570. [Q38954-1]
DR KEGG; ath:AT3G26570; -.
DR Araport; AT3G26570; -.
DR TAIR; locus:2088852; AT3G26570.
DR eggNOG; KOG2493; Eukaryota.
DR HOGENOM; CLU_015355_3_3_1; -.
DR InParanoid; Q38954; -.
DR OrthoDB; 712010at2759; -.
DR PhylomeDB; Q38954; -.
DR PRO; PR:Q38954; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q38954; baseline and differential.
DR Genevisible; Q38954; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0009673; F:low-affinity phosphate transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006817; P:phosphate ion transport; IMP:TAIR.
DR InterPro; IPR001204; Phos_transporter.
DR PANTHER; PTHR11101; PTHR11101; 1.
DR Pfam; PF01384; PHO4; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Membrane; Phosphate transport; Plastid;
KW Plastid inner membrane; Reference proteome; Symport; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..71
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 72..587
FT /note="Inorganic phosphate transporter 2-1, chloroplastic"
FT /id="PRO_0000042800"
FT TRANSMEM 127..147
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 154..174
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 327..347
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 413..433
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 465..485
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 523..543
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 559..579
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 74..106
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 86..103
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1
FT /note="M -> MQIHFHHHQYHHQLHSHHPWPISHLYM (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_018146"
SQ SEQUENCE 587 AA; 61419 MW; 21EFB76D0845C7D4 CRC64;
MTLPYRFSSV RNHSLLLKTS HLCTPRSALG CCFSPKESPF FRKNTAQFLS PQKHTSLPLK
LVCPLASFSS YADSEGEEQH HADQPIQNPH ESSTVSNESD GKGNAEATGD FSGMAQAFHI
SSTTARAISI VIAFSALTLP IFMKSLGQGL ALKTKLLSYA TLLFGFYMAW NIGANDVANA
MGTSVGSGAL TIRQAVMTAA VLEFSGALLM GTHVTSTMQK GILMANVFQG KDMLLFAGLL
SSLAAAGTWL QVASYYGWPV STTHCIVGSM VGFGLVYGGA GAVFWSSLAK VASSWVISPI
LGALVSFLVY KCIRRFVYSA PNPGQAAAAA APVAVFVGVA SISSAALPLS KIFPIALSQA
LACGVAGAIV FDRIIRKQLG HLLAKTKSPE TSQNQPKTIG FLSDIAGPTG TQLEIVYGIF
GYMQVLSACF MSFAHGGNDV SNAIGPLAAA LSILQNGAAA GGAEIVIPMD VLAWGGFGIV
AGLTMWGYRV IATIGKKITE LTPTRGFAAE FAAASVVLFA SKLGLPISAT HTLVGAVMGV
GFARGLNSVR AETVREIVAS WLVTIPVGAT LAVIYTWIFT KILSFVL
