ID   PHT2_ORYSJ              Reviewed;         319 AA.
AC   Q5SMQ0;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Putrescine hydroxycinnamoyltransferase 2 {ECO:0000305};
DE            Short=OsPHT2 {ECO:0000303|PubMed:27354554};
DE            EC=2.3.1.- {ECO:0000305};
GN   Name=PHT2 {ECO:0000303|PubMed:27354554};
GN   OrderedLocusNames=Os06g0185300 {ECO:0000312|EMBL:BAS96508.1},
GN   LOC_Os06g08610 {ECO:0000305};
GN   ORFNames=P0554A06.28 {ECO:0000312|EMBL:BAD72527.1};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   FUNCTION.
RX   PubMed=27354554; DOI=10.1105/tpc.16.00265;
RA   Peng M., Gao Y., Chen W., Wang W., Shen S., Shi J., Wang C., Zhang Y.,
RA   Zou L., Wang S., Wan J., Liu X., Gong L., Luo J.;
RT   "Evolutionarily distinct BAHD N-acyltransferases are responsible for
RT   natural variation of aromatic amine conjugates in rice.";
RL   Plant Cell 28:1533-1550(2016).
CC   -!- FUNCTION: Hydroxycinnamoyl transferase that catalyzes the transfer of
CC       an acyl from p-coumaryol-CoA to putrescine, to produce coumaroyl
CC       putrescine. {ECO:0000269|PubMed:27354554}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; AP005919; BAD72527.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAH93370.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS96508.1; -; Genomic_DNA.
DR   EMBL; AK109116; BAG98608.1; -; mRNA.
DR   RefSeq; XP_015641927.1; XM_015786441.1.
DR   AlphaFoldDB; Q5SMQ0; -.
DR   SMR; Q5SMQ0; -.
DR   STRING; 4530.OS06T0185300-01; -.
DR   PaxDb; Q5SMQ0; -.
DR   PRIDE; Q5SMQ0; -.
DR   EnsemblPlants; Os06t0185300-01; Os06t0185300-01; Os06g0185300.
DR   GeneID; 9268895; -.
DR   Gramene; Os06t0185300-01; Os06t0185300-01; Os06g0185300.
DR   KEGG; osa:9268895; -.
DR   eggNOG; ENOG502QTJX; Eukaryota.
DR   HOGENOM; CLU_014546_2_0_1; -.
DR   InParanoid; Q5SMQ0; -.
DR   OMA; CEAASTF; -.
DR   OrthoDB; 1130893at2759; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   ExpressionAtlas; Q5SMQ0; baseline and differential.
DR   GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR   GO; GO:0050734; F:hydroxycinnamoyltransferase activity; IDA:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..319
FT                   /note="Putrescine hydroxycinnamoyltransferase 2"
FT                   /id="PRO_0000437757"
FT   ACT_SITE        160
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT   ACT_SITE        301
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8W1W9"
SQ   SEQUENCE   319 AA;  34180 MW;  EBFECA3F711D8C32 CRC64;
     MATVEVLTSE VVAPAEETPA GAVWLSNLDL AARRGYTPTV YFYRRNGDDE AAFFAADAVR
     DGLARALVPF YPLAGRLGLA GGGEDGRVQI DCTGEGAVFV TARSGHYALD DLMNEFVPCD
     EMRDLFVPPT PPPNPPCALL LVQVTHLRCG GVVLGMALHH SVVDARSAAH FAETWASIVR
     GAPAGDAPVP PCFDHKLLAA RPARAVLYDH PEYKPEPAPA PAHAATASTY ASAIITLTKQ
     QVGALKAACA GASTFRAVVA LVWQCACRAR SLPPDKANLF LLYKYISEDW VDIQLNIQQR
     DNCIYAPTLK ANCCFTPTF