PHT3_ORYSJ
ID PHT3_ORYSJ Reviewed; 437 AA.
AC Q7XXN5;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Putrescine hydroxycinnamoyltransferase 3 {ECO:0000305};
DE Short=OsPHT3 {ECO:0000303|PubMed:27354554};
DE EC=2.3.1.- {ECO:0000305};
GN Name=PHT3 {ECO:0000303|PubMed:27015846};
GN OrderedLocusNames=Os09g0543900 {ECO:0000312|EMBL:BAT09225.1},
GN LOC_Os09g37180 {ECO:0000305};
GN ORFNames=P0705E11.3 {ECO:0000312|EMBL:BAC79154.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [5]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
RX PubMed=27015846; DOI=10.1111/jipb.12480;
RA Tanabe K., Hojo Y., Shinya T., Galis I.;
RT "Molecular evidence for biochemical diversification of phenolamide
RT biosynthesis in rice plants.";
RL J. Integr. Plant Biol. 58:903-913(2016).
RN [6]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=27354554; DOI=10.1105/tpc.16.00265;
RA Peng M., Gao Y., Chen W., Wang W., Shen S., Shi J., Wang C., Zhang Y.,
RA Zou L., Wang S., Wan J., Liu X., Gong L., Luo J.;
RT "Evolutionarily distinct BAHD N-acyltransferases are responsible for
RT natural variation of aromatic amine conjugates in rice.";
RL Plant Cell 28:1533-1550(2016).
CC -!- FUNCTION: Hydroxycinnamoyl transferase that catalyzes the transfer of
CC an acyl from p-coumaryol-CoA to putrescine, to produce coumaroyl
CC putrescine. Can use feruloyl-CoA and caffeoyl-CoA as acyl donors.
CC {ECO:0000269|PubMed:27354554}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=11.9 uM for p-coumaroyl-CoA {ECO:0000269|PubMed:27015846};
CC KM=23.7 uM for feruloyl-CoA {ECO:0000269|PubMed:27015846};
CC KM=80.8 uM for putrescine {ECO:0000269|PubMed:27015846};
CC -!- TISSUE SPECIFICITY: Highly expressed in roots. Expressed at low levels
CC in shoots and flowers. {ECO:0000269|PubMed:27015846}.
CC -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR EMBL; AP006548; BAC79154.1; -; Genomic_DNA.
DR EMBL; AP008215; BAF25742.1; -; Genomic_DNA.
DR EMBL; AP014965; BAT09225.1; -; Genomic_DNA.
DR EMBL; AK072013; BAG92784.1; -; mRNA.
DR EMBL; AK109723; BAG98872.1; -; mRNA.
DR RefSeq; XP_015651503.1; XM_015796017.1.
DR AlphaFoldDB; Q7XXN5; -.
DR SMR; Q7XXN5; -.
DR STRING; 4530.OS09T0543900-01; -.
DR PaxDb; Q7XXN5; -.
DR PRIDE; Q7XXN5; -.
DR EnsemblPlants; Os09t0543900-01; Os09t0543900-01; Os09g0543900.
DR EnsemblPlants; Os09t0543900-02; Os09t0543900-02; Os09g0543900.
DR GeneID; 4347747; -.
DR Gramene; Os09t0543900-01; Os09t0543900-01; Os09g0543900.
DR Gramene; Os09t0543900-02; Os09t0543900-02; Os09g0543900.
DR KEGG; osa:4347747; -.
DR eggNOG; ENOG502QTU2; Eukaryota.
DR HOGENOM; CLU_014546_6_2_1; -.
DR InParanoid; Q7XXN5; -.
DR OMA; PLIEYEH; -.
DR OrthoDB; 1130893at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR ExpressionAtlas; Q7XXN5; baseline and differential.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IBA:GO_Central.
DR GO; GO:0050734; F:hydroxycinnamoyltransferase activity; IDA:UniProtKB.
DR Gene3D; 3.30.559.10; -; 2.
DR InterPro; IPR023213; CAT-like_dom_sf.
PE 1: Evidence at protein level;
KW Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..437
FT /note="Putrescine hydroxycinnamoyltransferase 3"
FT /id="PRO_0000437758"
FT ACT_SITE 151
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
FT ACT_SITE 383
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8W1W9"
SQ SEQUENCE 437 AA; 47378 MW; D9F7F332E174FCD0 CRC64;
MEVKVLSSKL VKPAYNGGVA AAPDVEYIPL SIFDKVTYKM QMAIIYAFPP PAPSTAAIEK
GLAAVLAQYR AFAGQLGESP DGEAAVVLND RGARLVEAAV DADLVDMAPA KPTPELLRLH
PDLEGELQEV VLLQLTRFRC GSLAVGFTSN HVVADGHATS NFLVAWGRAT RGLPMGAPPV
HHHAALFKPR PSPHVEHDHR NREYYLPAAG DDSHGHGDGG AADNIVIHKA HFTKDFIAGL
RAAASEGRGR PFSRFETILA HLWRTMTRAR GLSPDEASTI RLSVDGRHRL GAPAEYFGNL
VLWAFPRATV GDLLTRPLKH AAQVIHDEVA RVDGAYFRSF LDFALSGAGG DKEGLAPSAV
LKDVLCPNAE VDSWLTFPFY ELDFGTGSPT YFMPSYFPTE GMLFLVPSYL GDGSVDAFVP
VFNHNLEAFK ECCYSME
