PHT43_ORYSJ
ID PHT43_ORYSJ Reviewed; 519 AA.
AC Q8W0H5; A0A0N7KE28; Q0JHN8; Q5N7A9;
DT 01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Probable anion transporter 3, chloroplastic;
DE AltName: Full=Phosphate transporter 4;3;
DE Flags: Precursor;
GN Name=PHT4;3; OrderedLocusNames=Os01g0852200, LOC_Os01g63290;
GN ORFNames=P0529E05.4-1, P0529E05.4-2, P0529H11.39-1, P0529H11.39-2;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19513231; DOI=10.4161/psb.3.10.6666;
RA Guo B., Irigoyen S., Fowler T.B., Versaw W.K.;
RT "Differential expression and phylogenetic analysis suggest specialization
RT of plastid-localized members of the PHT4 phosphate transporter family for
RT photosynthetic and heterotrophic tissues.";
RL Plant Signal. Behav. 3:784-790(2008).
CC -!- FUNCTION: Probable anion transporter. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8W0H5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8W0H5-2; Sequence=VSP_037958;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion
CC cotransporter (TC 2.A.1.14) family. {ECO:0000305}.
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DR EMBL; AP003279; BAB84388.1; -; Genomic_DNA.
DR EMBL; AP003279; BAD81937.1; -; Genomic_DNA.
DR EMBL; AP004072; BAB92858.1; -; Genomic_DNA.
DR EMBL; AP004072; BAD82655.1; -; Genomic_DNA.
DR EMBL; AP008207; BAF06740.2; -; Genomic_DNA.
DR EMBL; AP014957; BAS75257.1; -; Genomic_DNA.
DR EMBL; AK063954; BAG88934.1; -; mRNA.
DR EMBL; AK102621; BAG95643.1; -; mRNA.
DR RefSeq; XP_015620915.1; XM_015765429.1. [Q8W0H5-1]
DR AlphaFoldDB; Q8W0H5; -.
DR SMR; Q8W0H5; -.
DR IntAct; Q8W0H5; 1.
DR STRING; 4530.OS01T0852200-03; -.
DR PaxDb; Q8W0H5; -.
DR PRIDE; Q8W0H5; -.
DR EnsemblPlants; Os01t0852200-03; Os01t0852200-03; Os01g0852200. [Q8W0H5-1]
DR GeneID; 4324813; -.
DR Gramene; Os01t0852200-03; Os01t0852200-03; Os01g0852200. [Q8W0H5-1]
DR KEGG; osa:4324813; -.
DR eggNOG; KOG2532; Eukaryota.
DR InParanoid; Q8W0H5; -.
DR OMA; YNEQSQM; -.
DR OrthoDB; 497052at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR ExpressionAtlas; Q8W0H5; baseline and differential.
DR Genevisible; Q8W0H5; OS.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; IBA:GO_Central.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR CDD; cd17380; MFS_SLC17A9_like; 1.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR044777; SLC17A9-like.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Chloroplast; Ion transport; Membrane; Plastid;
KW Reference proteome; Transit peptide; Transmembrane; Transmembrane helix;
KW Transport.
FT TRANSIT 1..76
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 77..519
FT /note="Probable anion transporter 3, chloroplastic"
FT /id="PRO_0000383101"
FT TRANSMEM 100..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..273
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 326..346
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 460..480
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 488..508
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 56..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 56..75
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..166
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12869764"
FT /id="VSP_037958"
SQ SEQUENCE 519 AA; 55704 MW; 8F550577311CBC35 CRC64;
MAPPGQLLPL ARSLLPLSAP PFVSGRRRRL PTLVLGRALP PPTWLPHGRL SPAHPLPFAP
PRRLSRPPPP ATSLPGASPG GGAEAQAVLA EFVTSERVKV AAMLGLALAL CNADRVVMSV
AIVPLSQAYG WTPSFAGVVQ SSFLWGYLVS PIIGGALVDY YGGKRVMAYG VALWSLATFL
SPWAAARSLW LFLSTRVLLG MAEGVALPSM NNMVLRWFPR TERSSAVGIA MAGFQLGNTI
GLLLSPIIMS RAGIFGPFVI FGLFGFLWVL VWISAISGTP GENAQISAHE LDYITRGQKL
VKTQSGGERL RKVPPFSKLL SKWPTWALIS ANAMHSWGYF VILSWMPVYF KTIYHVNLRE
AAWFSALPWV MMAVLGYVAG VVSDRLIQNG TSITLTRKIM QTIGFVGPGV ALLGLNAAKS
PVIASAWLTI AVGLKSFGHS GFLVNLQEIA PQYAGVLHGM SNTAGTFAAI LGTVGAGFFV
DRMGSFRGFL ILTSLLYFSS TLFWDIFATG ERVDFDGTG
