PHTF2_MOUSE
ID PHTF2_MOUSE Reviewed; 747 AA.
AC Q8CB19; Q7TPX6;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 2.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Protein PHTF2 {ECO:0000305};
GN Name=Phtf2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
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DR EMBL; AK037021; BAC29673.1; -; mRNA.
DR EMBL; BC052841; AAH52841.1; -; mRNA.
DR CCDS; CCDS51426.1; -.
DR RefSeq; NP_766580.3; NM_172992.3.
DR AlphaFoldDB; Q8CB19; -.
DR BioGRID; 213041; 1.
DR STRING; 10090.ENSMUSP00000114087; -.
DR GlyGen; Q8CB19; 3 sites.
DR iPTMnet; Q8CB19; -.
DR PhosphoSitePlus; Q8CB19; -.
DR PaxDb; Q8CB19; -.
DR PRIDE; Q8CB19; -.
DR ProteomicsDB; 289555; -.
DR DNASU; 68770; -.
DR GeneID; 68770; -.
DR KEGG; mmu:68770; -.
DR UCSC; uc008wnz.2; mouse.
DR CTD; 57157; -.
DR MGI; MGI:1916020; Phtf2.
DR eggNOG; ENOG502QQGQ; Eukaryota.
DR InParanoid; Q8CB19; -.
DR OrthoDB; 710715at2759; -.
DR PhylomeDB; Q8CB19; -.
DR TreeFam; TF323570; -.
DR BioGRID-ORCS; 68770; 0 hits in 72 CRISPR screens.
DR ChiTaRS; Phtf2; mouse.
DR PRO; PR:Q8CB19; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q8CB19; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR039775; PHTF1/2.
DR InterPro; IPR021980; PHTF1/2_N.
DR PANTHER; PTHR12680; PTHR12680; 1.
DR Pfam; PF12129; Phtf-FEM1B_bdg; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..747
FT /note="Protein PHTF2"
FT /id="PRO_0000318510"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 126..146
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 459..479
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 515..535
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 596..616
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 630..650
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 722..742
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 6..153
FT /note="PHTF"
FT /evidence="ECO:0000255"
FT REGION 170..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..365
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 338..353
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 659
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 718
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 197
FT /note="Y -> H (in Ref. 1; BAC29673)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="L -> P (in Ref. 1; BAC29673)"
FT /evidence="ECO:0000305"
FT CONFLICT 366
FT /note="G -> D (in Ref. 1; BAC29673)"
FT /evidence="ECO:0000305"
FT CONFLICT 628
FT /note="C -> Y (in Ref. 1; BAC29673)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 747 AA; 84105 MW; 0994F8C14D29A889 CRC64;
MASRVTDAIV WYQKKIGAYD QQIWEKSVEQ REIKGLRNKP KKTAHVKPDL IDVDLVRGSA
FAKAKPESPW TSLTRKGIVR VVFFPFFSRW WLQVTSRVIF SWLLVLYLLQ VAAIVLFCSA
PSPHSIPLTE VIGPIWLMLL LGTVHCQIVS TRTPKPPLGT GGKRRRKLRK AAHLEVHREG
DGSSTTDNTQ EGAVQSYGAG APYSVGTVFR DLWLAAFFLS GSKKAKNSID KSTETDNGYV
SLDGKRTVKS SEDGAQYHEL QCETVGPEDA AWATRTPRSV PAKDTQRKIT NVSDEVSSEE
GPETGYPLRG HVDRTSESGL RNRKPHHYKK HYANEDAPKS GTSCSSRCSS SRQDSESTRP
ESETEGVLWE DLLHCAECRS SCTSETDVGN PQINPCGKKE YRDDPFHQSH LPWLHSSHPG
LEKISAIVWE GNDCKKADMS VLEISGMIMN RVNNHVPGIG YQVFGNAISL ILGLTPFVFR
LSQATDLEQL TAHSASELYV IAFGSNEDVM VLSMVLISFV VRVSLVWIFF FLLCVAERTY
KQRLLFAKLF GHLTSARRAR KSEVPHFRLK KVQNIKMWLS LRSYLKRRGP QRSVDVIVSS
AFLLTISVVF ICCAQLLHVH EIFLDCHCNW ELVIWCISLT LFLLRFVTLG SETSKKYSNT
SILLTEQINL YLKMEKKPNK KEELTLVNNV LKLATKLLKE LDSPFRLYGL TMNPLLYNIT
QVVILSAVSG VISDLLGFNL KLWKIKS
