PHTF_DROME
ID PHTF_DROME Reviewed; 880 AA.
AC Q9V9A8; Q9TW25;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 27-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Protein phtf {ECO:0000305};
GN Name=phtf; ORFNames=CG3268;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=10729229; DOI=10.1006/geno.1999.6079;
RA Manuel A., Beaupain D., Romeo P.-H., Raich N.;
RT "Molecular characterization of a novel gene family (PHTF) conserved from
RT Drosophila to mammals.";
RL Genomics 64:216-220(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-517 AND THR-527, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
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DR EMBL; AJ242938; CAB62168.1; -; mRNA.
DR EMBL; AE013599; AAM70823.1; -; Genomic_DNA.
DR EMBL; AF181638; AAD55424.1; -; mRNA.
DR RefSeq; NP_610232.1; NM_136388.3.
DR AlphaFoldDB; Q9V9A8; -.
DR BioGRID; 61479; 2.
DR DIP; DIP-23458N; -.
DR IntAct; Q9V9A8; 1.
DR STRING; 7227.FBpp0085459; -.
DR GlyGen; Q9V9A8; 4 sites.
DR iPTMnet; Q9V9A8; -.
DR PaxDb; Q9V9A8; -.
DR PRIDE; Q9V9A8; -.
DR EnsemblMetazoa; FBtr0086125; FBpp0085459; FBgn0028579.
DR GeneID; 35583; -.
DR KEGG; dme:Dmel_CG3268; -.
DR UCSC; CG3268-RA; d. melanogaster.
DR CTD; 35583; -.
DR FlyBase; FBgn0028579; phtf.
DR VEuPathDB; VectorBase:FBgn0028579; -.
DR eggNOG; ENOG502QQGQ; Eukaryota.
DR GeneTree; ENSGT00390000011648; -.
DR HOGENOM; CLU_013937_0_0_1; -.
DR InParanoid; Q9V9A8; -.
DR OMA; EMSGIIL; -.
DR OrthoDB; 710715at2759; -.
DR PhylomeDB; Q9V9A8; -.
DR BioGRID-ORCS; 35583; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35583; -.
DR PRO; PR:Q9V9A8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0028579; Expressed in testis and 28 other tissues.
DR Genevisible; Q9V9A8; DM.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR InterPro; IPR039775; PHTF1/2.
DR InterPro; IPR021980; PHTF1/2_N.
DR PANTHER; PTHR12680; PTHR12680; 1.
DR Pfam; PF12129; Phtf-FEM1B_bdg; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..880
FT /note="Protein phtf"
FT /id="PRO_0000127425"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 156..176
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 575..595
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 646..666
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 727..747
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 756..776
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 848..868
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 4..185
FT /note="PHTF"
FT /evidence="ECO:0000255"
FT REGION 256..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 357..412
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 434..464
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 264..286
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 392..406
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 434..458
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 517
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 527
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CARBOHYD 132
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 239
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 431
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 452
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 880 AA; 97635 MW; 3A39AC6A5BBB8E18 CRC64;
MKLDEIVAWY QKRIGTYDKQ EWEKTVEQRI LDGFNSVNLK NTKLKTELID VDLVRGSTFP
KAKPKQSLLT VIRLAILRYV LLPLYAQWWV KQTTPNAFGF ILVLYLTQLT NWAIYVLHSS
RIVPLDYEKP PNGTLLQAEA DGDASDKDAD KESEEHAALL SALLIPCALS LLISLIHSQI
VATNTASGVS GGSSKNKLRR ISASYLSDKA ATRENRVRRR KKIVRVRQVE ADLSQASSNI
SLPNRRTATS TIEVLPRPVT PLPSPTVTCA TVPDPTTPTT PSPSVIRRST NEETYLTTTA
ISPLTQPLAA IDACYDLSRK AGGAAPESPK KRNVNWHTPI QIYATYELGE EPCSSRKVAE
ESAPESVGER LCSVKPDYQT RRNIGEDDGF ESLNGKSSSG EDNNHSPLPN AVAVAAPPAP
VQTNQLRLRL NTTNGVTASA SPTEKKPQSR GNESSTSCAE SDECDDADIM SSPASGCNQE
CTTSATDWLG VTTNSEDCSY TSDLDHSDGG LKHTAFSDED PGELDITPTT ILNPHSSLDR
ISCTIWDQRD AKKAQLSVLE IASCIIERVD SMGEANDYIY IGVVFSFLLT LIPIFCRLCE
VTLGSDAEKA SEISYFNMPQ LLWEKSSASL FTLLGLAFGD SQWERMVLAL GFVQRLCLTL
ILFIIFAVAE RTFKQRFLYA KLFSHLTSSR RARKSNLPHF RLNKVRNIKT WLSVRSYLKK
RGPQRSVDII VSAAFIVTLL LLAFLSVEWL KDSAHLHTHL TLEALIWSIT IGIFLLRFMT
LGQKIQHKYR SVSVLITEQI NLYLQIEQKP KKKDELMVSN SVLKLAADLL KELETPFKLS
GLSANPYLFT TIKVVILSAL SGVLSEVLGF KLKLHKIKIK
