ID   PHYR_METEA              Reviewed;         267 AA.
AC   Q0PEV3; C5AW21;
DT   14-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 94.
DE   RecName: Full=Phyllosphere-induced regulator PhyR;
GN   Name=phyR; OrderedLocusNames=MexAM1_META1p3110;
OS   Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB
OS   9133 / AM1) (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=272630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RX   PubMed=16926146; DOI=10.1073/pnas.0603530103;
RA   Gourion B., Rossignol M., Vorholt J.A.;
RT   "A proteomic study of Methylobacterium extorquens reveals a response
RT   regulator essential for epiphytic growth.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:13186-13191(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1;
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA   Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA   Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA   Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA   Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA   Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA   Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to investigate
RT   microbial metabolism of C1 compounds from natural and industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
CC   -!- FUNCTION: Key regulator for adaptation to epiphytic life (leaf
CC       colonizing) of the bacterium. Positively regulates several genes
CC       including katE, sodA, hsp20, dps and gloA. However, it is not known
CC       whether this regulation is direct or indirect. Also induces several
CC       dehydrogenases.
CC   -!- INTERACTION:
CC       Q0PEV3; C5AW20: nepR; NbExp=2; IntAct=EBI-15757805, EBI-15757821;
CC   -!- DISRUPTION PHENOTYPE: The growth rate of the phyR deletion strain is
CC       the same as in the wild-type strain when plant colonization is mimicked
CC       in vitro in the presence of succinate and methanol. However, in planta
CC       colonization experiments show a severe growth defect of the phyR
CC       deletion mutant. {ECO:0000269|PubMed:16926146}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ACS40857.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; DQ845291; ABH05969.1; -; Genomic_DNA.
DR   EMBL; CP001510; ACS40857.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003599470.1; NC_012988.1.
DR   AlphaFoldDB; Q0PEV3; -.
DR   SMR; Q0PEV3; -.
DR   DIP; DIP-58555N; -.
DR   IntAct; Q0PEV3; 1.
DR   STRING; 272630.MexAM1_META1p3110; -.
DR   EnsemblBacteria; ACS40857; ACS40857; MexAM1_META1p3110.
DR   KEGG; mea:Mex_1p3110; -.
DR   eggNOG; COG0784; Bacteria.
DR   HOGENOM; CLU_089963_0_0_5; -.
DR   OrthoDB; 1133475at2; -.
DR   Proteomes; UP000009081; Chromosome.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR014605; Sig_resp-reg_PhyR.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF00072; Response_reg; 1.
DR   PIRSF; PIRSF036400; RR_Ctr_UCP036400; 1.
DR   SMART; SM00448; REC; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   1: Evidence at protein level;
KW   Phosphoprotein; Transcription; Transcription regulation.
FT   CHAIN           1..267
FT                   /note="Phyllosphere-induced regulator PhyR"
FT                   /id="PRO_0000259889"
FT   DOMAIN          139..253
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   MOD_RES         190
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   267 AA;  29141 MW;  7CFA51F6D09E536D CRC64;
     MSTAQLVVQH LPYLRRYARA LTGSQVAGDA YVAATLETLV NEPETLGRST NVKADLFRVF
     TRIWNSLSVN GHSDQVQHDL PAEVRLGQIT PLPRQAFLLS CLEGFSEEDA GVILDVDVSK
     VRDLVDEAGR ELAADMATEI LIIEDEPLIA MDLEALVEGL GHNVIGVART RTEAVKIASE
     SKRPGLILAD IQLADGSSGL DAVNDLLKTF EVPVIFITAY PERFLTGERP EPAFLIAKPF
     QPANVSAVIS QALFFQQSAR RREAHNA