PHYT_BACSU
ID PHYT_BACSU Reviewed; 382 AA.
AC P42094;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 2.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=3-phytase;
DE EC=3.1.3.8;
DE AltName: Full=Myo-inositol-hexaphosphate 3-phosphohydrolase;
DE AltName: Full=Phytate 3-phosphatase;
DE Flags: Precursor;
GN Name=phy; Synonyms=yodV, yzxA; OrderedLocusNames=BSU19800;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9734814; DOI=10.1093/dnares/5.3.195;
RA Ghim S.-Y., Choi S.-K., Shin B.-S., Jeong Y.-M., Sorokin A., Ehrlich S.D.,
RA Park S.-H.;
RT "Sequence analysis of the Bacillus subtilis 168 chromosome region between
RT the sspC and odhA loci (184 degrees-180 degrees).";
RL DNA Res. 5:195-201(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 206-382.
RC STRAIN=168;
RX PubMed=7592393; DOI=10.1128/jb.177.21.6263-6275.1995;
RA Roels S., Losick R.;
RT "Adjacent and divergently oriented operons under the control of the
RT sporulation regulatory protein GerE in Bacillus subtilis.";
RL J. Bacteriol. 177:6263-6275(1995).
RN [4]
RP CHARACTERIZATION.
RA Farouk A., Borriss R.;
RT "Gene identification in bacterial genomes: isolation of phytase encoding
RT genes in Bacillus subtilis and Clostridium acetobutylicum.";
RL (In) Proceedings of Genome 2000: international conference of microbial and
RL model genomes, pp.41-42, Institut Pasteur, Paris (2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1D-myo-inositol hexakisphosphate + H2O = 1D-myo-inositol
CC 1,2,4,5,6-pentakisphosphate + phosphate; Xref=Rhea:RHEA:16989,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:57798,
CC ChEBI:CHEBI:58130; EC=3.1.3.8; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU00997};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
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DR EMBL; AF015775; AAB72078.1; -; Genomic_DNA.
DR EMBL; AF006665; AAB81148.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13871.1; -; Genomic_DNA.
DR EMBL; U18421; AAA87722.1; -; Genomic_DNA.
DR PIR; H69904; H69904.
DR RefSeq; NP_389861.1; NC_000964.3.
DR RefSeq; WP_003230820.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P42094; -.
DR SMR; P42094; -.
DR STRING; 224308.BSU19800; -.
DR PaxDb; P42094; -.
DR PRIDE; P42094; -.
DR EnsemblBacteria; CAB13871; CAB13871; BSU_19800.
DR GeneID; 940065; -.
DR KEGG; bsu:BSU19800; -.
DR PATRIC; fig|224308.179.peg.2169; -.
DR eggNOG; COG4247; Bacteria.
DR InParanoid; P42094; -.
DR OMA; GLTIYYA; -.
DR PhylomeDB; P42094; -.
DR BioCyc; BSUB:BSU19800-MON; -.
DR BRENDA; 3.1.3.8; 658.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016158; F:3-phytase activity; IEA:UniProtKB-EC.
DR Gene3D; 2.120.10.30; -; 1.
DR InterPro; IPR011042; 6-blade_b-propeller_TolB-like.
DR InterPro; IPR003431; BP_Phytase.
DR Pfam; PF02333; Phytase; 1.
DR PROSITE; PS51662; BP_PHYTASE; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Reference proteome; Secreted; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000255"
FT CHAIN 27..382
FT /note="3-phytase"
FT /id="PRO_0000022060"
FT DOMAIN 27..361
FT /note="BPP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00997"
SQ SEQUENCE 382 AA; 41946 MW; BDB30045F9D162E4 CRC64;
MKVPKTMLLS TAAGLLLSLT ATSVSAHYVN EEHHFKVTAH TETDPVASGD DAADDPAIWV
HEKHPEKSKL ITTNKKSGLV VYDLDGKQLH SYEFGKLNNV DLRYDFPLNG EKIDIAAASN
RSEGKNTIEV YAIDGDKGKL KSITDPNHPI STNISEVYGF SLYHSQKTGA FYALVTGKQG
EFEQYEIVDG GKGYVTGKKV REFKLNSQTE GLVADDEYGN LYIAEEDEAI WKFNAEPGGG
SKGQVVDRAT GDHLTADIEG LTIYYAPNGK GYLMASSQGN NSYAMYERQG KNRYVANFEI
TDGEKIDGTS DTDGIDVLGF GLGPKYPYGI FVAQDGENID NGQAVNQNFK IVSWEQIAQH
LGEMPDLHKQ VNPRKLKDRS DG
