ID   PHY_EUBRA               Reviewed;         274 AA.
AC   Q715L4;
DT   01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 30.
DE   RecName: Full=Phloretin hydrolase {ECO:0000303|PubMed:15466559};
DE            EC=3.7.1.4 {ECO:0000269|PubMed:15466559};
GN   Name=phy;
OS   Eubacterium ramulus.
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae;
OC   Eubacterium.
OX   NCBI_TaxID=39490;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY,
RP   SUBCELLULAR LOCATION, SUBUNIT, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=15466559; DOI=10.1128/aem.70.10.6131-6137.2004;
RA   Schoefer L., Braune A., Blaut M.;
RT   "Cloning and expression of a phloretin hydrolase gene from Eubacterium
RT   ramulus and characterization of the recombinant enzyme.";
RL   Appl. Environ. Microbiol. 70:6131-6137(2004).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=24826896; DOI=10.1371/journal.pone.0097250;
RA   Shearer A.G., Altman T., Rhee C.D.;
RT   "Finding sequences for over 270 orphan enzymes.";
RL   PLoS ONE 9:E97250-E97250(2014).
CC   -!- FUNCTION: Catalyzes the hydrolytic C-C cleavage of phloretin to
CC       phloroglucinol and 3-(4-hydroxyphenyl)propionic acid during flavonoid
CC       degradation. Also hydrolyzes other C-acylated phenols.
CC       {ECO:0000269|PubMed:15466559}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + phloretin = 1,3,5-trihydroxybenzene + H(+) + phloretate;
CC         Xref=Rhea:RHEA:23396, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16204, ChEBI:CHEBI:16331, ChEBI:CHEBI:17276; EC=3.7.1.4;
CC         Evidence={ECO:0000269|PubMed:15466559};
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000250|UniProtKB:Q4K423};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=13 uM for phloretin {ECO:0000269|PubMed:15466559};
CC         Note=kcat is 10 sec(-1) for phloretin. {ECO:0000269|PubMed:15466559};
CC       pH dependence:
CC         Optimum pH is 7.0. {ECO:0000269|PubMed:15466559};
CC       Temperature dependence:
CC         Optimum temperature is 37 degrees Celsius.
CC         {ECO:0000269|PubMed:15466559};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:15466559}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:15466559}.
CC   -!- SIMILARITY: Belongs to the DAPG/phloretin hydrolase family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF548616; AAQ12341.1; -; Genomic_DNA.
DR   STRING; 39490.ERS852448_03037; -.
DR   KEGG; ag:AAQ12341; -.
DR   BRENDA; 3.7.1.4; 17131.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0050180; F:phloretin hydrolase activity; IDA:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR   GO; GO:0046275; P:flavonoid catabolic process; IDA:UniProtKB.
DR   InterPro; IPR041526; DAPG_hydrolase.
DR   Pfam; PF18089; DAPG_hydrolase; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Hydrolase; Metal-binding; Zinc.
FT   CHAIN           1..274
FT                   /note="Phloretin hydrolase"
FT                   /id="PRO_0000430447"
FT   BINDING         123
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q4K423"
FT   BINDING         154
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q4K423"
FT   BINDING         251
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q4K423"
FT   BINDING         255
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000250|UniProtKB:Q4K423"
SQ   SEQUENCE   274 AA;  30923 MW;  FB19CA698ACE3297 CRC64;
     MEEDFNMSTP GVKVGVXEEE KKLSYYKYYE QDLAPVPAEK IAILQGGPIA PEKCIPFDER
     NKFLKGEDDE YANIGFGVAA DGTALVCNTT YMPGVTGEML DWWFPWHSVG SDLRYKIWDP
     EDHYFARAYP ASYVVDPNVP MNQKTWGVDH YIMEDVGPGP EFLKLCFKRP ADFGYDESII
     GTEKCESLVC AIGESSCAAA MTHKWHPYKD GVLFESRFWI GYRIDEEGNI VKAIPEGVSI
     PPFVPQGLFA HNIKEFTNLA AILPTLYAEE KDTF