ID   PHZA_PSECL              Reviewed;         207 AA.
AC   Q51518;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Probable isochorismatase;
DE            EC=3.3.2.1;
DE   AltName: Full=2,3 dihydro-2,3 dihydroxybenzoate synthase;
GN   Name=phzA;
OS   Pseudomonas chlororaphis (Pseudomonas aureofaciens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=30-84;
RX   PubMed=8586283; DOI=10.1111/j.1574-6968.1995.tb07954.x;
RA   Pierson L.S. III, Gaffney T., Lam S., Gong F.;
RT   "Molecular analysis of genes encoding phenazine biosynthesis in the
RT   biological control bacterium. Pseudomonas aureofaciens 30-84.";
RL   FEMS Microbiol. Lett. 134:299-307(1995).
CC   -!- FUNCTION: Involved in the biosynthesis of the antibiotic phenazine, a
CC       nitrogen-containing heterocyclic molecule having important roles in
CC       virulence, competition and biological control. This isochorismatase may
CC       remove pyruvate from chorismate during the formation of the phenazine
CC       ring structure and/or stabilize the phenazine biosynthetic complex.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC         dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC   -!- PATHWAY: Antibiotic biosynthesis; phenazine biosynthesis.
CC   -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000305}.
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DR   EMBL; L48339; AAB00329.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q51518; -.
DR   SMR; Q51518; -.
DR   PRIDE; Q51518; -.
DR   UniPathway; UPA00099; -.
DR   GO; GO:0008908; F:isochorismatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0002047; P:phenazine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.850; -; 1.
DR   InterPro; IPR016291; Isochorismatase.
DR   InterPro; IPR000868; Isochorismatase-like.
DR   InterPro; IPR036380; Isochorismatase-like_sf.
DR   Pfam; PF00857; Isochorismatase; 1.
DR   PIRSF; PIRSF001111; Isochorismatase; 1.
DR   PRINTS; PR01398; ISCHRISMTASE.
DR   SUPFAM; SSF52499; SSF52499; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Hydrolase; Virulence.
FT   CHAIN           1..207
FT                   /note="Probable isochorismatase"
FT                   /id="PRO_0000201825"
SQ   SEQUENCE   207 AA;  23086 MW;  E86F1073366E13F5 CRC64;
     MTGIPSIVPY ALPTNRDLPV NLAQWSIDPE RAVLLVHDMQ RYFLRPLPDA LRDEVVSNAA
     RIRQWAADNG VPVAYTAQPG SMSEEQRGLL KDFWGPGMKA SPADREVVGA LTPKPGDWLL
     TKWRYSAFFN SDLLERMRAN GRDQLILCGV YAHVGVLIST VDAYSNDIQP FLVADAIADF
     SKEHHWMAIE YAASRCAMVI TTDEVVL