ID   PHZF_PSEAE              Reviewed;         278 AA.
AC   O69754;
DT   26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 2.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Trans-2,3-dihydro-3-hydroxyanthranilate isomerase;
DE            EC=5.3.3.17 {ECO:0000250|UniProtKB:Q51792};
DE   AltName: Full=Phenazine/pyocyanine biosynthesis protein PhzF;
GN   Name=phzF1; Synonyms=phzF; OrderedLocusNames=PA1904;
GN   and
GN   Name=phzF2; OrderedLocusNames=PA4215;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RA   Mavrodi D.V., Delaney S.M., Thomashow L.S.;
RT   "New genes involved in pyocyanine biosynthesis in Pseudomonas aeruginosa
RT   PAO1.";
RL   Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: Isomerase that catalyzes the condensation of two molecules of
CC       trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine
CC       ring system. The final product is not yet known.
CC       {ECO:0000250|UniProtKB:Q51792}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxyate =
CC         (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate;
CC         Xref=Rhea:RHEA:28182, ChEBI:CHEBI:60849, ChEBI:CHEBI:60862;
CC         EC=5.3.3.17; Evidence={ECO:0000250|UniProtKB:Q51792};
CC   -!- PATHWAY: Secondary metabolite biosynthesis; pyocyanine biosynthesis.
CC   -!- SIMILARITY: Belongs to the PhzF family. {ECO:0000305}.
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DR   EMBL; AF005404; AAC64492.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG05293.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG07602.1; -; Genomic_DNA.
DR   PIR; B83119; B83119.
DR   RefSeq; NP_250595.1; NC_002516.2.
DR   RefSeq; NP_252904.1; NC_002516.2.
DR   RefSeq; WP_003093628.1; NZ_QZGE01000046.1.
DR   AlphaFoldDB; O69754; -.
DR   SMR; O69754; -.
DR   STRING; 287.DR97_3696; -.
DR   PaxDb; O69754; -.
DR   PRIDE; O69754; -.
DR   DNASU; 879183; -.
DR   EnsemblBacteria; AAG05293; AAG05293; PA1904.
DR   EnsemblBacteria; AAG07602; AAG07602; PA4215.
DR   GeneID; 879183; -.
DR   GeneID; 880481; -.
DR   KEGG; pae:PA1904; -.
DR   KEGG; pae:PA4215; -.
DR   PATRIC; fig|208964.12.peg.1982; -.
DR   PseudoCAP; PA1904; -.
DR   PseudoCAP; PA4215; -.
DR   HOGENOM; CLU_048756_0_1_6; -.
DR   InParanoid; O69754; -.
DR   OMA; FHVRDPF; -.
DR   PhylomeDB; O69754; -.
DR   UniPathway; UPA00235; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0016853; F:isomerase activity; IBA:GO_Central.
DR   GO; GO:0102943; F:trans-2,3-dihydro-3-hydroxy-anthranilate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0002047; P:phenazine biosynthetic process; IDA:PseudoCAP.
DR   InterPro; IPR003719; Phenazine_PhzF.
DR   PANTHER; PTHR13774; PTHR13774; 1.
DR   Pfam; PF02567; PhzC-PhzF; 1.
DR   PIRSF; PIRSF016184; PhzC_PhzF; 1.
DR   TIGRFAMs; TIGR00654; PhzF_family; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Isomerase; Reference proteome.
FT   CHAIN           1..278
FT                   /note="Trans-2,3-dihydro-3-hydroxyanthranilate isomerase"
FT                   /id="PRO_0000162380"
FT   ACT_SITE        45
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   278 AA;  30343 MW;  6099983CC6B30DC3 CRC64;
     MHRYVVIDAF ASEPLQGNPV AVFFDCDDLS GERMQRMARE MNLSESTFVL RPQQDGDARI
     RIFTPVNELP FAGHPLLGTA IALGAETDKD RLFLETRMGT VPFALERQDG KVVACSMQQP
     IPTWEHFSRP AELLAALGLK GSTFPIEVYR NGPRHVFVGL ESVAALSALH PDHRALCDFP
     DLAVNCFAGA GRHWRSRMFS PAYGVVEDAA TGSAAGPLAI HLARHRQIPY GQQIEILQGV
     EIGRPSRMYA RAEGAGERVS AVEVSGNGAA FAEGRAYL