ID   PHZF_PSECL              Reviewed;         278 AA.
AC   Q51520; Q9R9G2;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   26-SEP-2001, sequence version 2.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Trans-2,3-dihydro-3-hydroxyanthranilate isomerase;
DE            EC=5.3.3.17 {ECO:0000250|UniProtKB:Q51792};
DE   AltName: Full=Phenazine/pyocyanine biosynthesis protein PhzF;
GN   Name=phzF; Synonyms=phzC;
OS   Pseudomonas chlororaphis (Pseudomonas aureofaciens).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=30-84;
RX   PubMed=8586283; DOI=10.1111/j.1574-6968.1995.tb07954.x;
RA   Pierson L.S. III, Gaffney T., Lam S., Gong F.;
RT   "Molecular analysis of genes encoding phenazine biosynthesis in the
RT   biological control bacterium. Pseudomonas aureofaciens 30-84.";
RL   FEMS Microbiol. Lett. 134:299-307(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PCL1391;
RX   PubMed=11497469; DOI=10.1094/mpmi.2001.14.8.969;
RA   Chin-A-Woeng T.F.C., van den Broek D., de Voer G., van der Drift K.M.,
RA   Tuinman S., Thomas-Oates J.E., Lugtenberg B.J.J., Bloemberg G.V.;
RT   "Phenazine-1-carboxamide production in the biocontrol strain Pseudomonas
RT   chlororaphis PCL1391 is regulated by multiple factors secreted into the
RT   growth medium.";
RL   Mol. Plant Microbe Interact. 14:969-979(2001).
CC   -!- FUNCTION: Isomerase that catalyzes the condensation of two molecules of
CC       trans-2,3-dihydro-3-hydroxyanthranilic acid (DHHA) into the phenazine
CC       ring system. The final product is not yet known.
CC       {ECO:0000250|UniProtKB:Q51792}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxyate =
CC         (1R,6S)-6-amino-5-oxocyclohex-2-ene-1-carboxylate;
CC         Xref=Rhea:RHEA:28182, ChEBI:CHEBI:60849, ChEBI:CHEBI:60862;
CC         EC=5.3.3.17; Evidence={ECO:0000250|UniProtKB:Q51792};
CC   -!- PATHWAY: Antibiotic biosynthesis; phenazine biosynthesis.
CC   -!- SIMILARITY: Belongs to the PhzF family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; L48339; AAB00331.1; -; Genomic_DNA.
DR   EMBL; AF195615; AAF17500.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q51520; -.
DR   SMR; Q51520; -.
DR   UniPathway; UPA00099; -.
DR   GO; GO:0102943; F:trans-2,3-dihydro-3-hydroxy-anthranilate isomerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0002047; P:phenazine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR003719; Phenazine_PhzF.
DR   PANTHER; PTHR13774; PTHR13774; 1.
DR   Pfam; PF02567; PhzC-PhzF; 1.
DR   PIRSF; PIRSF016184; PhzC_PhzF; 1.
DR   TIGRFAMs; TIGR00654; PhzF_family; 1.
PE   3: Inferred from homology;
KW   Antibiotic biosynthesis; Isomerase; Virulence.
FT   CHAIN           1..278
FT                   /note="Trans-2,3-dihydro-3-hydroxyanthranilate isomerase"
FT                   /id="PRO_0000162381"
FT   ACT_SITE        45
FT                   /evidence="ECO:0000250"
FT   CONFLICT        2
FT                   /note="H -> E (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        13
FT                   /note="A -> V (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        21
FT                   /note="T -> A (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        32
FT                   /note="T -> E (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        80
FT                   /note="A -> D (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        86
FT                   /note="H -> R (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        113
FT                   /note="V -> I (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        131
FT                   /note="V -> A (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        177
FT                   /note="S -> Y (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        269
FT                   /note="V -> I (in Ref. 1; AAB00331)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   278 AA;  30118 MW;  88BB229A45F04C06 CRC64;
     MHHYVIIDAF ASAPLEGNPV TVFFDADDLS ATQMQRIARE MNLSETTFVL KPRNCGDALI
     RIFTPVNELP FAGHPLLGTA IALGAHTDNH RLFLETQMGT IAFELERQNG SVVAASMDQP
     IPTWTALGRD VELLKALGIS DSTFPIEIYH NGPRHVFVGL PSIAALSALH PDHRALSSFH
     DMAINCFAGA GRRWRSRMFS PAYGVVEDAA TGSAAGPLAI HLARHGQIEF GQQIEILQGV
     EIGRPSLMFA RAEGRADQLT RVEVSGNGVT FGRGTIVL