PI15_HUMAN
ID PI15_HUMAN Reviewed; 258 AA.
AC O43692; Q68CY1;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Peptidase inhibitor 15;
DE Short=PI-15;
DE AltName: Full=25 kDa trypsin inhibitor;
DE Short=p25TI;
DE AltName: Full=Cysteine-rich secretory protein 8;
DE Short=CRISP-8;
DE AltName: Full=SugarCrisp;
DE Flags: Precursor;
GN Name=PI15; Synonyms=CRISP8, P25TI;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND GLYCOSYLATION.
RX PubMed=9473672; DOI=10.1016/s0167-4781(97)00149-8;
RA Yamakawa T., Miyata S., Ogawa N., Koshikawa N., Yasumitsu H., Kanamori T.,
RA Miyazaki K.;
RT "cDNA cloning of a novel trypsin inhibitor with similarity to pathogenesis-
RT related proteins, and its frequent expression in human brain cancer
RT cells.";
RL Biochim. Biophys. Acta 1395:202-208(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Endometrial adenocarcinoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 61-85, FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=8882727; DOI=10.1093/oxfordjournals.jbchem.a021244;
RA Koshikawa N., Nakamura T., Tsuchiya N., Isaji M., Yasumitsu H., Umeda M.,
RA Miyazaki K.;
RT "Purification and identification of a novel and four known serine
RT proteinase inhibitors secreted by human glioblastoma cells.";
RL J. Biochem. 119:334-339(1996).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=11287197; DOI=10.1016/s0925-4773(01)00293-3;
RA Smith D.M., Collins-Racie L.A., Marigo V.A., Roberts D.J., Davis N.M.,
RA Hartmann C., Schweitzer R., LaVallie E.R., Gamer L., McCoy J., Tabin C.J.;
RT "Cloning and expression of a novel cysteine-rich secreted protein family
RT member expressed in thyroid and pancreatic mesoderm within the chicken
RT embryo.";
RL Mech. Dev. 102:223-226(2001).
CC -!- FUNCTION: Serine protease inhibitor which displays weak inhibitory
CC activity against trypsin (PubMed:8882727). May play a role in facial
CC patterning during embryonic development (By similarity).
CC {ECO:0000250|UniProtKB:Q98ST6, ECO:0000269|PubMed:8882727}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8882727}.
CC -!- TISSUE SPECIFICITY: Weakly expressed. Expressed at low level in
CC prostate, mammary gland, salivary gland and thyroid gland.
CC {ECO:0000269|PubMed:11287197, ECO:0000269|PubMed:9473672}.
CC -!- PTM: N-glycosylated. {ECO:0000305|PubMed:9473672}.
CC -!- SIMILARITY: Belongs to the CRISP family. {ECO:0000305}.
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DR EMBL; D45027; BAA25066.1; -; mRNA.
DR EMBL; CR749657; CAH18451.2; -; mRNA.
DR EMBL; BC074931; AAH74931.1; -; mRNA.
DR EMBL; BC074932; AAH74932.1; -; mRNA.
DR EMBL; BC126290; AAI26291.1; -; mRNA.
DR EMBL; BC126292; AAI26293.1; -; mRNA.
DR CCDS; CCDS6218.1; -.
DR RefSeq; NP_001311332.1; NM_001324403.1.
DR RefSeq; NP_056970.1; NM_015886.4.
DR AlphaFoldDB; O43692; -.
DR SMR; O43692; -.
DR BioGRID; 119244; 91.
DR STRING; 9606.ENSP00000260113; -.
DR GlyGen; O43692; 3 sites.
DR iPTMnet; O43692; -.
DR PhosphoSitePlus; O43692; -.
DR BioMuta; PI15; -.
DR jPOST; O43692; -.
DR MassIVE; O43692; -.
DR MaxQB; O43692; -.
DR PaxDb; O43692; -.
DR PeptideAtlas; O43692; -.
DR PRIDE; O43692; -.
DR ProteomicsDB; 49119; -.
DR Antibodypedia; 1731; 184 antibodies from 28 providers.
DR DNASU; 51050; -.
DR Ensembl; ENST00000260113.7; ENSP00000260113.2; ENSG00000137558.9.
DR Ensembl; ENST00000523773.1; ENSP00000428567.1; ENSG00000137558.9.
DR Ensembl; ENST00000649643.1; ENSP00000497041.1; ENSG00000137558.9.
DR GeneID; 51050; -.
DR KEGG; hsa:51050; -.
DR MANE-Select; ENST00000260113.7; ENSP00000260113.2; NM_015886.5; NP_056970.1.
DR UCSC; uc003yal.3; human.
DR CTD; 51050; -.
DR DisGeNET; 51050; -.
DR GeneCards; PI15; -.
DR HGNC; HGNC:8946; PI15.
DR HPA; ENSG00000137558; Tissue enhanced (lymphoid tissue, prostate, smooth muscle).
DR MIM; 607076; gene.
DR neXtProt; NX_O43692; -.
DR OpenTargets; ENSG00000137558; -.
DR PharmGKB; PA33282; -.
DR VEuPathDB; HostDB:ENSG00000137558; -.
DR eggNOG; KOG3017; Eukaryota.
DR GeneTree; ENSGT00940000158635; -.
DR HOGENOM; CLU_035730_2_2_1; -.
DR InParanoid; O43692; -.
DR OMA; CSPNEVY; -.
DR OrthoDB; 1528782at2759; -.
DR PhylomeDB; O43692; -.
DR TreeFam; TF316148; -.
DR PathwayCommons; O43692; -.
DR BioGRID-ORCS; 51050; 8 hits in 1068 CRISPR screens.
DR ChiTaRS; PI15; human.
DR GenomeRNAi; 51050; -.
DR Pharos; O43692; Tbio.
DR PRO; PR:O43692; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; O43692; protein.
DR Bgee; ENSG00000137558; Expressed in cauda epididymis and 129 other tissues.
DR Genevisible; O43692; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0030414; F:peptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0010466; P:negative regulation of peptidase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.40.33.10; -; 1.
DR InterPro; IPR018244; Allrgn_V5/Tpx1_CS.
DR InterPro; IPR014044; CAP_domain.
DR InterPro; IPR035940; CAP_sf.
DR InterPro; IPR001283; CRISP-related.
DR PANTHER; PTHR10334; PTHR10334; 1.
DR Pfam; PF00188; CAP; 1.
DR PRINTS; PR00837; V5TPXLIKE.
DR SMART; SM00198; SCP; 1.
DR SUPFAM; SSF55797; SSF55797; 1.
DR PROSITE; PS01010; CRISP_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Direct protein sequencing; Glycoprotein;
KW Protease inhibitor; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..60
FT /evidence="ECO:0000305|PubMed:8882727"
FT /id="PRO_0000287622"
FT CHAIN 61..258
FT /note="Peptidase inhibitor 15"
FT /id="PRO_0000287623"
FT DOMAIN 71..211
FT /note="SCP"
FT CARBOHYD 26
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 258 AA; 29065 MW; 1915A5831637795F CRC64;
MIAISAVSSA LLFSLLCEAS TVVLLNSTDS SPPTNNFTDI EAALKAQLDS ADIPKARRKR
YISQNDMIAI LDYHNQVRGK VFPPAANMEY MVWDENLAKS AEAWAATCIW DHGPSYLLRF
LGQNLSVRTG RYRSILQLVK PWYDEVKDYA FPYPQDCNPR CPMRCFGPMC THYTQMVWAT
SNRIGCAIHT CQNMNVWGSV WRRAVYLVCN YAPKGNWIGE APYKVGVPCS SCPPSYGGSC
TDNLCFPGVT SNYLYWFK
