PI21_ORYSJ
ID PI21_ORYSJ Reviewed; 266 AA.
AC Q7XL73; B5UA05; B9FEZ5; Q0JDI8;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Protein PYRICULARIA ORYZAE RESISTANCE 21 {ECO:0000303|PubMed:19696351};
DE Short=Pi21 {ECO:0000303|PubMed:19696351};
GN Name=PI21 {ECO:0000303|PubMed:19696351}; Synonyms=rMg40;
GN OrderedLocusNames=LOC_Os04g32850,
GN Os04g0401000 {ECO:0000312|EMBL:BAF14599.1};
GN ORFNames=OsJ_14657 {ECO:0000312|EMBL:EEE60928.1},
GN OSJNBb0014D23.16 {ECO:0000312|EMBL:CAE05282.1},
GN OSNPB_040401000 {ECO:0000312|EMBL:BAS89039.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2), FUNCTION, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=cv. Aichi asahi;
RX PubMed=19696351; DOI=10.1126/science.1175550;
RA Fukuoka S., Saka N., Koga H., Ono K., Shimizu T., Ebana K., Hayashi N.,
RA Takahashi A., Hirochika H., Okuno K., Yano M.;
RT "Loss of function of a proline-containing protein confers durable disease
RT resistance in rice.";
RL Science 325:998-1001(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [8]
RP REVIEW.
RX PubMed=18533827; DOI=10.1094/mpmi-21-7-0859;
RA Ballini E., Morel J.-B., Droc G., Price A., Courtois B., Notteghem J.-L.,
RA Tharreau D.;
RT "A genome-wide meta-analysis of rice blast resistance genes and
RT quantitative trait loci provides new insights into partial and complete
RT resistance.";
RL Mol. Plant Microbe Interact. 21:859-868(2008).
RN [9]
RP REVIEW.
RX PubMed=23184051; DOI=10.1007/s11033-012-2318-0;
RA Miah G., Rafii M.Y., Ismail M.R., Puteh A.B., Rahim H.A., Asfaliza R.,
RA Latif M.A.;
RT "Blast resistance in rice: a review of conventional breeding to molecular
RT approaches.";
RL Mol. Biol. Rep. 40:2369-2388(2013).
RN [10]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Aichi asahi;
RX PubMed=26740780; DOI=10.1080/13102818.2014.978664;
RA Wang D., Qin Y., Han J., Zhang L., Xu X., Liu X., Wang C., Liu X.;
RT "Expression analysis of innate immunity related genes in the true/field
RT blast resistance gene-mediated defence response.";
RL Biotechnol. Biotechnol. Equip. 28:999-1007(2014).
RN [11]
RP INDUCTION BY MAGNAPORTHE ORYZAE.
RX PubMed=26001124; DOI=10.1371/journal.pone.0126188;
RA Azizi P., Rafii M.Y., Mahmood M., Abdullah S.N., Hanafi M.M., Nejat N.,
RA Latif M.A., Sahebi M.;
RT "Differential gene expression reflects morphological characteristics and
RT physiological processes in rice immunity against blast pathogen Magnaporthe
RT oryzae.";
RL PLoS ONE 10:E0126188-E0126188(2015).
CC -!- FUNCTION: Involved in defense responses (PubMed:19696351,
CC PubMed:26740780). Contributes to slowing defense responses toward
CC Magnaporthe oryzae (PubMed:19696351). {ECO:0000269|PubMed:19696351,
CC ECO:0000269|PubMed:26740780}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q7XL73-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q7XL73-2; Sequence=VSP_058514, VSP_058515;
CC Name=3;
CC IsoId=Q7XL73-3; Sequence=VSP_058516;
CC -!- INDUCTION: By Magnaporthe oryzae. {ECO:0000269|PubMed:26001124}.
CC -!- DISRUPTION PHENOTYPE: The pi21 allele confers an improved non-race-
CC specific blast resistance toward Magnaporthe oryzae (PubMed:19696351).
CC Reduced salicylic acid (SA) responses (e.g. WRKY45 expression) but
CC increased systemic acquired response (SAR, e.g. PR1b and PBZ1
CC accumulation), jasmonic acid (JA, e.g. JAmyb expression) and abscisic
CC acid (ABA, e.g. SalT expression) responses in response to M.oryzae
CC spores (PubMed:26740780). {ECO:0000269|PubMed:19696351,
CC ECO:0000269|PubMed:26740780}.
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DR EMBL; AB430852; BAG72122.1; -; Genomic_DNA.
DR EMBL; AB430853; BAG72123.1; -; Genomic_DNA.
DR EMBL; AL731642; CAE05282.1; -; Genomic_DNA.
DR EMBL; AP008210; BAF14599.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS89038.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS89039.1; -; Genomic_DNA.
DR EMBL; CM000141; EEE60928.1; -; Genomic_DNA.
DR EMBL; AK070581; BAG92042.1; -; mRNA.
DR RefSeq; XP_015634053.1; XM_015778567.1. [Q7XL73-1]
DR AlphaFoldDB; Q7XL73; -.
DR SMR; Q7XL73; -.
DR STRING; 4530.OS04T0401000-01; -.
DR PaxDb; Q7XL73; -.
DR PRIDE; Q7XL73; -.
DR EnsemblPlants; Os04t0401000-01; Os04t0401000-01; Os04g0401000. [Q7XL73-1]
DR EnsemblPlants; Os04t0401000-02; Os04t0401000-02; Os04g0401000. [Q7XL73-3]
DR GeneID; 4335725; -.
DR Gramene; Os04t0401000-01; Os04t0401000-01; Os04g0401000. [Q7XL73-1]
DR Gramene; Os04t0401000-02; Os04t0401000-02; Os04g0401000. [Q7XL73-3]
DR KEGG; osa:4335725; -.
DR eggNOG; ENOG502SFJM; Eukaryota.
DR InParanoid; Q7XL73; -.
DR OMA; SGGHGNC; -.
DR OrthoDB; 1567388at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0050832; P:defense response to fungus; IEP:UniProtKB.
DR GO; GO:1900150; P:regulation of defense response to fungus; IMP:UniProtKB.
DR InterPro; IPR044169; PI21.
DR PANTHER; PTHR47488; PTHR47488; 1.
DR PROSITE; PS50846; HMA_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Metal-binding; Plant defense; Reference proteome.
FT CHAIN 1..266
FT /note="Protein PYRICULARIA ORYZAE RESISTANCE 21"
FT /id="PRO_0000437419"
FT DOMAIN 1..68
FT /note="HMA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT REGION 129..156
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..155
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 12
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT BINDING 15
FT /ligand="a metal cation"
FT /ligand_id="ChEBI:CHEBI:25213"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00280"
FT VAR_SEQ 77..83
FT /note="Missing (in isoform 2)"
FT /id="VSP_058514"
FT VAR_SEQ 136..151
FT /note="Missing (in isoform 2)"
FT /id="VSP_058515"
FT VAR_SEQ 187..242
FT /note="Missing (in isoform 3)"
FT /id="VSP_058516"
FT CONFLICT 245
FT /note="P -> R (in Ref. 6; EEE60928)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 266 AA; 29479 MW; 5D3B36107DCF23BF CRC64;
MGILVILVDL QCCRCDAKIR KVLGCLEEEY CIEKVEYDVK NNRVIVRGKF DPEKLCKKIW
CKAGKIIKEI LIVDVWPPPL PQPPPPCKPP PCEKPPEDCK PKPCHCCSCE KPKPKPKPCH
CEKPKPCHCE KPKPCEKPPP CKPEEPPKPP PEKPPPKPEC KLVPYPYPVP YPYAGQWCCP
KPEPPKPPPE PPKEPEPPKP CGCSHAFVCV CKPAPPPPPP CGCSGGHGNC GCGIRPWPPQ
VWPPPPVCPP PPWCYTEDNA NACSIM
