PI2R_RAT
ID PI2R_RAT Reviewed; 416 AA.
AC P43253;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Prostacyclin receptor;
DE AltName: Full=Prostaglandin I2 receptor;
DE Short=PGI receptor;
DE Short=PGI2 receptor;
DE AltName: Full=Prostanoid IP receptor;
DE Flags: Precursor;
GN Name=Ptgir;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lung;
RX PubMed=7803522; DOI=10.1016/0167-4889(94)90300-x;
RA Sasaki Y., Usui T., Tanaka I., Nakagawa O., Sando T., Takahashi T.,
RA Namba T., Narumiya S., Nakao K.;
RT "Cloning and expression of a cDNA for rat prostacyclin receptor.";
RL Biochim. Biophys. Acta 1224:601-605(1994).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-366, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Receptor for prostacyclin (prostaglandin I2 or PGI2). The
CC activity of this receptor is mediated by G(s) proteins which activate
CC adenylate cyclase.
CC -!- SUBUNIT: Interacts (non-isoprenylated C-terminus) with PDZK1.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- PTM: Isoprenylation does not influence ligand binding but is required
CC for efficient coupling to the effectors adenylyl cyclase and
CC phospholipase C. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
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DR EMBL; D28966; BAA06091.1; -; mRNA.
DR PIR; S52078; S52078.
DR RefSeq; NP_001071112.1; NM_001077644.1.
DR AlphaFoldDB; P43253; -.
DR SMR; P43253; -.
DR STRING; 10116.ENSRNOP00000022461; -.
DR BindingDB; P43253; -.
DR ChEMBL; CHEMBL3322; -.
DR DrugCentral; P43253; -.
DR GuidetoPHARMACOLOGY; 345; -.
DR GlyGen; P43253; 1 site.
DR iPTMnet; P43253; -.
DR PhosphoSitePlus; P43253; -.
DR PaxDb; P43253; -.
DR PRIDE; P43253; -.
DR Ensembl; ENSRNOT00000022461; ENSRNOP00000022461; ENSRNOG00000016756.
DR GeneID; 292661; -.
DR KEGG; rno:292661; -.
DR UCSC; RGD:1310890; rat.
DR CTD; 5739; -.
DR RGD; 1310890; Ptgir.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244902; -.
DR HOGENOM; CLU_045991_0_1_1; -.
DR InParanoid; P43253; -.
DR OMA; IHPFCGD; -.
DR OrthoDB; 972015at2759; -.
DR PhylomeDB; P43253; -.
DR Reactome; R-RNO-391908; Prostanoid ligand receptors.
DR Reactome; R-RNO-392851; Prostacyclin signalling through prostacyclin receptor.
DR PRO; PR:P43253; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000016756; Expressed in lung and 16 other tissues.
DR GO; GO:0005829; C:cytosol; ISO:RGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISO:RGD.
DR GO; GO:0016501; F:prostacyclin receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; ISO:RGD.
DR GO; GO:0006954; P:inflammatory response; IBA:GO_Central.
DR GO; GO:0010642; P:negative regulation of platelet-derived growth factor receptor signaling pathway; ISO:RGD.
DR GO; GO:0048662; P:negative regulation of smooth muscle cell proliferation; ISO:RGD.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IBA:GO_Central.
DR GO; GO:0051239; P:regulation of multicellular organismal process; IEA:UniProt.
DR GO; GO:0032496; P:response to lipopolysaccharide; ISO:RGD.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR008365; Prostanoid_rcpt.
DR InterPro; IPR000370; Prostglndn_IP_rcpt.
DR PANTHER; PTHR11866; PTHR11866; 1.
DR PANTHER; PTHR11866:SF7; PTHR11866:SF7; 1.
DR Pfam; PF00001; 7tm_1; 1.
DR PRINTS; PR01788; PROSTANOIDR.
DR PRINTS; PR00856; PRSTNOIDIPR.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Lipoprotein; Membrane; Methylation; Phosphoprotein; Prenylation; Receptor;
KW Reference proteome; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..413
FT /note="Prostacyclin receptor"
FT /id="PRO_0000070077"
FT PROPEP 414..416
FT /note="Removed in mature form"
FT /evidence="ECO:0000255"
FT /id="PRO_0000240006"
FT TOPO_DOM 1..45
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 46..67
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..80
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 81..105
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..187
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..215
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 216..237
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 238..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..289
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..302
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 366
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT MOD_RES 413
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 413
FT /note="S-farnesyl cysteine"
FT /evidence="ECO:0000250"
FT CARBOHYD 36
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 34..194
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT DISULFID 121..199
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
SQ SEQUENCE 416 AA; 44662 MW; 03171B5ED21C4171 CRC64;
MVASGGRPDG PPSITPESPL IVGGREWQGM AGSCWNITYV QDSVGPATST LMFVAGVVGN
GLALGILGAR RRSHPSAFAV LVTGLAVTDL LGTCFLSPAV FVAYARNSSL LGLAHGGTML
CDTFAFAMTF FGLASTLILF AMAVERCLAL SHPYLYAQLD GPRCARLALP AIYAFCCLFC
SLPLLGLGEH QQYCPGSWCF IRMRSPQPGG CAFSLAYASL MALLVTSIFF CNGSVTLSLC
HMYRQQRRHH GSFVPTSRAR EDEVYHLILL ALMTGIMAVC SLPLTIRGFT QAIAPDSREM
GDLHAFRFNA FNPILDPWVF ILFRKAVFQR LKFWLCCLCA RSVHGDLQTP LSRPVSGRRD
TLAPDSLQAK EGNWVPLSTW GTGQVAPLTA VPLSGGDGCS VGMPSKTEAV VACSLC
