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PI4KA_HUMAN
ID   PI4KA_HUMAN             Reviewed;        2102 AA.
AC   P42356; Q7Z625; Q9UPG2;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   17-FEB-2016, sequence version 4.
DT   03-AUG-2022, entry version 199.
DE   RecName: Full=Phosphatidylinositol 4-kinase alpha;
DE            Short=PI4-kinase alpha;
DE            Short=PI4K-alpha;
DE            Short=PtdIns-4-kinase alpha;
DE            EC=2.7.1.67 {ECO:0000269|PubMed:10101268};
DE   AltName: Full=Phosphatidylinositol 4-Kinase III alpha;
GN   Name=PI4KA; Synonyms=PIK4, PIK4CA;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), TISSUE SPECIFICITY, AND ACTIVITY
RP   REGULATION.
RX   PubMed=7961848; DOI=10.1016/s0021-9258(19)61989-7;
RA   Wong K., Cantley L.C.;
RT   "Cloning and characterization of a human phosphatidylinositol 4-kinase.";
RL   J. Biol. Chem. 269:28878-28884(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10591208; DOI=10.1038/990031;
RA   Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M.,
RA   Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C.,
RA   Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E.,
RA   Bridgeman A.M., Buck D., Burgess J., Burrill W.D., Burton J., Carder C.,
RA   Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G.,
RA   Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V.,
RA   Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M.,
RA   Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A.,
RA   Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C.,
RA   Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E.,
RA   Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F.,
RA   Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M.,
RA   Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A.,
RA   Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D.,
RA   Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y.,
RA   Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S.,
RA   Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E.,
RA   Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L.,
RA   Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L.,
RA   Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N.,
RA   Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A.,
RA   Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L.,
RA   Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P.,
RA   Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P.,
RA   Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q.,
RA   Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J.,
RA   Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J.,
RA   Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D.,
RA   Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T.,
RA   Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P.,
RA   Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K.,
RA   Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R.,
RA   Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L.,
RA   McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J.,
RA   Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E.,
RA   Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P.,
RA   Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y.,
RA   Wright H.;
RT   "The DNA sequence of human chromosome 22.";
RL   Nature 402:489-495(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 59-2102 (ISOFORM 1), FUNCTION, CATALYTIC
RP   ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, AND TISSUE SPECIFICITY.
RX   PubMed=10101268; DOI=10.1016/s1388-1981(99)00029-3;
RA   Gehrmann T., Guelkan H., Suer S., Herberg F.W., Balla A., Vereb G. Jr.,
RA   Mayr G.W., Heilmeyer L.M.G. Jr.;
RT   "Functional expression and characterisation of a new human
RT   phosphatidylinositol 4-kinase PI4K230.";
RL   Biochim. Biophys. Acta 1437:341-356(1999).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1200-2102 (ISOFORM 1).
RC   TISSUE=Uterus;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-265, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18691976; DOI=10.1016/j.molcel.2008.07.007;
RA   Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R.,
RA   Greff Z., Keri G., Stemmann O., Mann M.;
RT   "Kinase-selective enrichment enables quantitative phosphoproteomics of the
RT   kinome across the cell cycle.";
RL   Mol. Cell 31:438-448(2008).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-257; SER-260; SER-262 AND
RP   SER-265, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA   Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA   Elledge S.J., Gygi S.P.;
RT   "A quantitative atlas of mitotic phosphorylation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19369195; DOI=10.1074/mcp.m800588-mcp200;
RA   Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G.,
RA   Mann M., Daub H.;
RT   "Large-scale proteomics analysis of the human kinome.";
RL   Mol. Cell. Proteomics 8:1751-1764(2009).
RN   [8]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-265 AND SER-1436, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19690332; DOI=10.1126/scisignal.2000007;
RA   Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K.,
RA   Rodionov V., Han D.K.;
RT   "Quantitative phosphoproteomic analysis of T cell receptor signaling
RT   reveals system-wide modulation of protein-protein interactions.";
RL   Sci. Signal. 2:RA46-RA46(2009).
RN   [9]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA   Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [10]
RP   GENE STRUCTURE, FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE PI4K
RP   COMPLEX, AND ACTIVITY REGULATION.
RX   PubMed=23229899; DOI=10.1083/jcb.201206095;
RA   Nakatsu F., Baskin J.M., Chung J., Tanner L.B., Shui G., Lee S.Y.,
RA   Pirruccello M., Hao M., Ingolia N.T., Wenk M.R., De Camilli P.;
RT   "PtdIns4P synthesis by PI4KIIIalpha at the plasma membrane and its impact
RT   on plasma membrane identity.";
RL   J. Cell Biol. 199:1003-1016(2012).
RN   [11]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-230; SER-256; SER-257;
RP   SER-262; SER-265; SER-429; TYR-1154 AND SER-1436, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma, and Erythroleukemia;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
RN   [12]
RP   SUBCELLULAR LOCATION, AND INTERACTION WITH TTC7A.
RX   PubMed=24417819; DOI=10.1053/j.gastro.2014.01.015;
RA   Avitzur Y., Guo C., Mastropaolo L.A., Bahrami E., Chen H., Zhao Z.,
RA   Elkadri A., Dhillon S., Murchie R., Fattouh R., Huynh H., Walker J.L.,
RA   Wales P.W., Cutz E., Kakuta Y., Dudley J., Kammermeier J., Powrie F.,
RA   Shah N., Walz C., Nathrath M., Kotlarz D., Puchaka J., Krieger J.R.,
RA   Racek T., Kirchner T., Walters T.D., Brumell J.H., Griffiths A.M.,
RA   Rezaei N., Rashtian P., Najafi M., Monajemzadeh M., Pelsue S.,
RA   McGovern D.P., Uhlig H.H., Schadt E., Klein C., Snapper S.B., Muise A.M.;
RT   "Mutations in tetratricopeptide repeat domain 7A result in a severe form of
RT   very early onset inflammatory bowel disease.";
RL   Gastroenterology 146:1028-1039(2014).
RN   [13]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1436, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Liver;
RX   PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA   Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA   Ye M., Zou H.;
RT   "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT   phosphoproteome.";
RL   J. Proteomics 96:253-262(2014).
RN   [14]
RP   INVOLVEMENT IN NEDSPLB, VARIANTS NEDSPLB 796-ARG--TYR-2102 DEL AND
RP   ASN-1854, AND CHARACTERIZATION OF VARIANT NEDSPLB ASN-1854.
RX   PubMed=25855803; DOI=10.1093/hmg/ddv117;
RA   Pagnamenta A.T., Howard M.F., Wisniewski E., Popitsch N., Knight S.J.,
RA   Keays D.A., Quaghebeur G., Cox H., Cox P., Balla T., Taylor J.C., Kini U.;
RT   "Germline recessive mutations in PI4KA are associated with perisylvian
RT   polymicrogyria, cerebellar hypoplasia and arthrogryposis.";
RL   Hum. Mol. Genet. 24:3732-3741(2015).
RN   [15]
RP   INTERACTION WITH TMEM150A, SUBCELLULAR LOCATION, AND ACTIVITY REGULATION.
RX   PubMed=25608530; DOI=10.15252/embr.201439151;
RA   Chung J., Nakatsu F., Baskin J.M., De Camilli P.;
RT   "Plasticity of PI4KIIIalpha interactions at the plasma membrane.";
RL   EMBO Rep. 16:312-320(2015).
RN   [16]
RP   IDENTIFICATION IN THE PI4K COMPLEX, AND ACTIVITY REGULATION.
RX   PubMed=26571211; DOI=10.1038/ncb3271;
RA   Baskin J.M., Wu X., Christiano R., Oh M.S., Schauder C.M., Gazzerro E.,
RA   Messa M., Baldassari S., Assereto S., Biancheri R., Zara F., Minetti C.,
RA   Raimondi A., Simons M., Walther T.C., Reinisch K.M., De Camilli P.;
RT   "The leukodystrophy protein FAM126A (hyccin) regulates PtdIns(4)P synthesis
RT   at the plasma membrane.";
RL   Nat. Cell Biol. 18:132-138(2016).
RN   [17]
RP   IDENTIFICATION IN A COMPLEX WITH CHKA AND HCV NON-STRUCTURAL PROTEIN 5A
RP   (MICROBIAL INFECTION).
RX   PubMed=28566381; DOI=10.1128/jvi.00355-17;
RA   Wong M.T., Chen S.S.;
RT   "Hepatitis C Virus Subverts Human Choline Kinase-alpha To Bridge
RT   Phosphatidylinositol-4-Kinase IIIalpha (PI4KIIIalpha) and NS5A and
RT   Upregulates PI4KIIIalpha Activation, Thereby Promoting the Translocation of
RT   the Ternary Complex to the Endoplasmic Reticulum for Viral Replication.";
RL   J. Virol. 91:0-0(2017).
RN   [18]
RP   VARIANTS NEDSPLB TRP-119; ARG-472; 618-ARG--TYR-2102 DEL; LYS-1152;
RP   THR-1198; ARG-1295; ASN-1664; ASN-1854; ARG-1925; SER-1987 AND THR-2041,
RP   VARIANTS SPG84 MET-1556; ILE-1720 AND GLU-1820 DEL, INVOLVEMENT IN NEDSPLB,
RP   AND INVOLVEMENT IN SPG84.
RX   PubMed=34415322; DOI=10.1093/brain/awab124;
RA   Verdura E., Rodriguez-Palmero A., Velez-Santamaria V., Planas-Serra L.,
RA   de la Calle I., Raspall-Chaure M., Roubertie A., Benkirane M., Saettini F.,
RA   Pavinato L., Mandrile G., O'Leary M., O'Heir E., Barredo E., Chacon A.,
RA   Michaud V., Goizet C., Ruiz M., Schlueter A., Rouvet I., Sala-Coromina J.,
RA   Fossati C., Iascone M., Canonico F., Marce-Grau A., de Souza P.,
RA   Adams D.R., Casasnovas C., Rehm H.L., Mefford H.C.,
RA   Gonzalez Gutierrez-Solana L., Brusco A., Koenig M., Macaya A., Pujol A.;
RT   "Biallelic PI4KA variants cause a novel neurodevelopmental syndrome with
RT   hypomyelinating leukodystrophy.";
RL   Brain 144:2659-2669(2021).
RN   [19]
RP   VARIANTS NEDSPLB 566-ARG--TYR-2102 DEL; PRO-777; 1191-GLN--TYR-2102 DEL;
RP   TRP-1733; THR-1808; ASN-1854; GLU-1925 AND CYS-1937, VARIANT GIDID2
RP   ASP-1623, CHARACTERIZATION OF VARIANT GIDID2 ASP-1623, INTERACTION WITH
RP   TTC7A, INVOLVEMENT IN NEDSPLB, INVOLVEMENT IN GIDID2, AND MUTAGENESIS OF
RP   ASP-1957.
RX   PubMed=34415310; DOI=10.1093/brain/awab313;
RA   Salter C.G., Cai Y., Lo B., Helman G., Taylor H., McCartney A.,
RA   Leslie J.S., Accogli A., Zara F., Traverso M., Fasham J., Lees J.A.,
RA   Ferla M.P., Chioza B.A., Wenger O., Scott E., Cross H.E., Crawford J.,
RA   Warshawsky I., Keisling M., Agamanolis D., Ward Melver C., Cox H.,
RA   Elawad M., Marton T., Wakeling M.N., Holzinger D., Tippelt S., Munteanu M.,
RA   Valcheva D., Deal C., Van Meerbeke S., Walsh Vockley C., Butte M.J.,
RA   Acar U., van der Knaap M.S., Korenke G.C., Kotzaeridou U., Balla T.,
RA   Simons C., Uhlig H.H., Crosby A.H., De Camilli P., Wolf N.I., Baple E.L.;
RT   "Biallelic PI4KA variants cause neurological, intestinal and immunological
RT   disease.";
RL   Brain 144:3597-3610(2021).
CC   -!- FUNCTION: Acts on phosphatidylinositol (PtdIns) in the first committed
CC       step in the production of the second messenger inositol-1,4,5,-
CC       trisphosphate. {ECO:0000269|PubMed:10101268,
CC       ECO:0000269|PubMed:23229899}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a
CC         1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate) + ADP +
CC         H(+); Xref=Rhea:RHEA:19877, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57880, ChEBI:CHEBI:58178, ChEBI:CHEBI:456216;
CC         EC=2.7.1.67; Evidence={ECO:0000269|PubMed:10101268};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:19878;
CC         Evidence={ECO:0000305|PubMed:7961848};
CC   -!- ACTIVITY REGULATION: Activated by Triton X-100, insensitive to
CC       inhibition by adenosine and inhibited by wortmannin (By similarity).
CC       Isoform 2 is activated by detergents such as Triton X-100 and inhibited
CC       by adenosine (PubMed:7961848). The PI4K complex acts as a regulator of
CC       phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis
CC       (PubMed:23229899, PubMed:26571211). Interaction with TMEM150A regulates
CC       PtdIns(4)P synthesis (PubMed:25608530). {ECO:0000250|UniProtKB:O08662,
CC       ECO:0000269|PubMed:23229899, ECO:0000269|PubMed:25608530,
CC       ECO:0000269|PubMed:26571211, ECO:0000269|PubMed:7961848}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=300 uM for ATP {ECO:0000269|PubMed:10101268};
CC   -!- SUBUNIT: Component of a phosphatidylinositol 4-kinase (PI4K) complex,
CC       composed of PI4KA, EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or TTC7B) and
CC       FAM126 (FAM126A or FAM126B) (PubMed:23229899, PubMed:24417819,
CC       PubMed:26571211, PubMed:34415310). Interacts with TMEM150A; regulating
CC       recruitment to the plasma membrane (PubMed:25608530). Interacts with
CC       TTC7A (PubMed:34415310). {ECO:0000269|PubMed:23229899,
CC       ECO:0000269|PubMed:24417819, ECO:0000269|PubMed:25608530,
CC       ECO:0000269|PubMed:26571211, ECO:0000269|PubMed:34415310}.
CC   -!- SUBUNIT: (Microbial infection) Interacts with CHKA/Choline Kinase-
CC       alpha; CHKA bridges PI4KA and hepatitis C virus (HCV) non-structural
CC       protein 5A (NS5A) and potentiates NS5A-stimulated PI4KA activity, which
CC       then facilitates the targeting of the ternary complex to the ER for
CC       viral replication. {ECO:0000269|PubMed:28566381}.
CC   -!- INTERACTION:
CC       P42356; PRO_0000037576 [P27958]; Xeno; NbExp=7; IntAct=EBI-723050, EBI-8753518;
CC       P42356; PRO_0000045602 [Q99IB8]; Xeno; NbExp=5; IntAct=EBI-723050, EBI-6927873;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23229899,
CC       ECO:0000269|PubMed:24417819}. Cell membrane
CC       {ECO:0000269|PubMed:23229899, ECO:0000269|PubMed:24417819}.
CC       Note=Localization to the plasma membrane is mediated by the PI4K
CC       complex and association with EFR3 (EFR3A or EFR3B), TTC7 (TTC7A or
CC       TTC7B) and FAM126 (FAM126A or FAM126B) (PubMed:23229899). Localization
CC       to the plasma membrane is regulated by TMEM150A (PubMed:25608530).
CC       {ECO:0000269|PubMed:23229899, ECO:0000269|PubMed:25608530}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1; Synonyms=PI4K230 {ECO:0000303|PubMed:10101268};
CC         IsoId=P42356-1; Sequence=Displayed;
CC       Name=2; Synonyms=PI4K97 {ECO:0000303|PubMed:10101268};
CC         IsoId=P42356-2; Sequence=VSP_008805;
CC   -!- TISSUE SPECIFICITY: Expressed ubiquitously. Highest levels in placenta
CC       and brain. Little or no expression in lung, liver, pancreas, testis or
CC       leukocytes. {ECO:0000269|PubMed:10101268, ECO:0000269|PubMed:7961848}.
CC   -!- DISEASE: Neurodevelopmental disorder with spasticity, hypomyelinating
CC       leukodystrophy, and brain abnormalities (NEDSPLB) [MIM:616531]: A
CC       severe autosomal recessive disorder characterized by global
CC       developmental delay with impaired intellectual development and poor or
CC       absent speech, axial hypotonia, and peripheral spasticity and
CC       hyperreflexia. Brain imaging shows hypomyelination with decreased white
CC       matter volume, cerebral and cerebellar atrophy, and thin corpus
CC       callosum. Polymicrogyria may be observed in rare cases. Some patients
CC       have a primary immunodeficiency or gastrointestinal disturbances
CC       similar to inflammatory bowel disease. {ECO:0000269|PubMed:25855803,
CC       ECO:0000269|PubMed:34415310, ECO:0000269|PubMed:34415322}. Note=The
CC       disease is caused by variants affecting the gene represented in this
CC       entry.
CC   -!- DISEASE: Gastrointestinal defects and immunodeficiency syndrome 2
CC       (GIDID2) [MIM:619708]: A severe autosomal recessive disorder
CC       characterized by multiple intestinal atresia apparent soon after birth.
CC       Affected infants have a distended abdomen, bowel obstruction and do not
CC       pass meconium. There is some evidence of inflammatory bowel disease.
CC       Death occurs in the first weeks of life. Some patients may also have
CC       immunodeficiency. {ECO:0000269|PubMed:34415310}. Note=The disease is
CC       caused by variants affecting the gene represented in this entry.
CC   -!- DISEASE: Spastic paraplegia 84, autosomal recessive (SPG84)
CC       [MIM:619621]: A form of spastic paraplegia, a neurodegenerative
CC       disorder characterized by a slow, gradual, progressive weakness and
CC       spasticity of the lower limbs. Rate of progression and the severity of
CC       symptoms are quite variable. Initial symptoms may include difficulty
CC       with balance, weakness and stiffness in the legs, muscle spasms, and
CC       dragging the toes when walking. In some forms of the disorder, bladder
CC       symptoms (such as incontinence) may appear, or the weakness and
CC       stiffness may spread to other parts of the body. SPG84 is characterized
CC       by onset of slowly progressive walking difficulties due to lower limb
CC       weakness, stiffness, and spasticity in the first 2 decades of life.
CC       {ECO:0000269|PubMed:34415322}. Note=The disease is caused by variants
CC       affecting the gene represented in this entry.
CC   -!- SIMILARITY: Belongs to the PI3/PI4-kinase family. Type III PI4K
CC       subfamily. {ECO:0000305}.
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DR   EMBL; L36151; AAA56839.1; -; mRNA.
DR   EMBL; AC007050; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AC007308; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AF012872; AAD13352.1; -; mRNA.
DR   EMBL; BC018120; AAH18120.2; -; mRNA.
DR   EMBL; BC053654; AAH53654.1; -; mRNA.
DR   CCDS; CCDS33603.2; -. [P42356-1]
DR   PIR; A55404; A55404.
DR   RefSeq; NP_477352.3; NM_058004.3. [P42356-1]
DR   PDB; 6BQ1; EM; 3.60 A; A/E=932-2102.
DR   PDBsum; 6BQ1; -.
DR   AlphaFoldDB; P42356; -.
DR   SMR; P42356; -.
DR   CORUM; P42356; -.
DR   IntAct; P42356; 89.
DR   MINT; P42356; -.
DR   STRING; 9606.ENSP00000255882; -.
DR   BindingDB; P42356; -.
DR   ChEMBL; CHEMBL3667; -.
DR   DrugCentral; P42356; -.
DR   GuidetoPHARMACOLOGY; 2148; -.
DR   SwissLipids; SLP:000000895; -.
DR   SwissLipids; SLP:000000902; -. [P42356-2]
DR   iPTMnet; P42356; -.
DR   MetOSite; P42356; -.
DR   PhosphoSitePlus; P42356; -.
DR   SwissPalm; P42356; -.
DR   BioMuta; PI4KA; -.
DR   DMDM; 218512114; -.
DR   EPD; P42356; -.
DR   jPOST; P42356; -.
DR   MassIVE; P42356; -.
DR   MaxQB; P42356; -.
DR   PaxDb; P42356; -.
DR   PeptideAtlas; P42356; -.
DR   PRIDE; P42356; -.
DR   ProteomicsDB; 55512; -. [P42356-1]
DR   ProteomicsDB; 55513; -. [P42356-2]
DR   Antibodypedia; 34781; 55 antibodies from 19 providers.
DR   DNASU; 5297; -.
DR   Ensembl; ENST00000255882.11; ENSP00000255882.6; ENSG00000241973.11. [P42356-1]
DR   GeneID; 5297; -.
DR   KEGG; hsa:5297; -.
DR   MANE-Select; ENST00000255882.11; ENSP00000255882.6; NM_058004.4; NP_477352.3.
DR   CTD; 5297; -.
DR   DisGeNET; 5297; -.
DR   GeneCards; PI4KA; -.
DR   HGNC; HGNC:8983; PI4KA.
DR   HPA; ENSG00000241973; Low tissue specificity.
DR   MalaCards; PI4KA; -.
DR   MIM; 600286; gene.
DR   MIM; 616531; phenotype.
DR   MIM; 619621; phenotype.
DR   MIM; 619708; phenotype.
DR   neXtProt; NX_P42356; -.
DR   OpenTargets; ENSG00000241973; -.
DR   Orphanet; 98889; Bilateral perisylvian polymicrogyria.
DR   Orphanet; 436252; Combined immunodeficiency-enteropathy spectrum.
DR   PharmGKB; PA162399305; -.
DR   VEuPathDB; HostDB:ENSG00000241973; -.
DR   eggNOG; KOG0902; Eukaryota.
DR   GeneTree; ENSGT00550000074798; -.
DR   InParanoid; P42356; -.
DR   OMA; QEYRYSE; -.
DR   OrthoDB; 1147978at2759; -.
DR   PhylomeDB; P42356; -.
DR   TreeFam; TF102041; -.
DR   BioCyc; MetaCyc:HS13481-MON; -.
DR   PathwayCommons; P42356; -.
DR   Reactome; R-HSA-1483248; Synthesis of PIPs at the ER membrane.
DR   Reactome; R-HSA-1660514; Synthesis of PIPs at the Golgi membrane.
DR   SABIO-RK; P42356; -.
DR   SignaLink; P42356; -.
DR   BioGRID-ORCS; 5297; 430 hits in 1091 CRISPR screens.
DR   ChiTaRS; PI4KA; human.
DR   GeneWiki; PI4KA; -.
DR   GenomeRNAi; 5297; -.
DR   Pharos; P42356; Tchem.
DR   PRO; PR:P42356; -.
DR   Proteomes; UP000005640; Chromosome 22.
DR   RNAct; P42356; protein.
DR   Bgee; ENSG00000241973; Expressed in superior frontal gyrus and 94 other tissues.
DR   ExpressionAtlas; P42356; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR   GO; GO:0005829; C:cytosol; TAS:Reactome.
DR   GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR   GO; GO:0005925; C:focal adhesion; HDA:UniProtKB.
DR   GO; GO:0030660; C:Golgi-associated vesicle membrane; ISS:UniProtKB.
DR   GO; GO:0016020; C:membrane; HDA:UniProtKB.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0004430; F:1-phosphatidylinositol 4-kinase activity; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0045296; F:cadherin binding; HDA:BHF-UCL.
DR   GO; GO:0016301; F:kinase activity; IDA:MGI.
DR   GO; GO:0052742; F:phosphatidylinositol kinase activity; IBA:GO_Central.
DR   GO; GO:0044788; P:modulation by host of viral process; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
DR   GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; ISS:UniProtKB.
DR   GO; GO:0048015; P:phosphatidylinositol-mediated signaling; IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IDA:MGI.
DR   GO; GO:0140754; P:reorganization of cellular membranes to establish viral sites of replication; IMP:ParkinsonsUK-UCL.
DR   GO; GO:0007165; P:signal transduction; NAS:ProtInc.
DR   Gene3D; 1.10.1070.11; -; 1.
DR   Gene3D; 1.25.40.70; -; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR   InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR   InterPro; IPR018936; PI3/4_kinase_CS.
DR   InterPro; IPR001263; PI3K_accessory_dom.
DR   InterPro; IPR042236; PI3K_accessory_sf.
DR   InterPro; IPR045495; PI4K_N.
DR   InterPro; IPR015433; PI_Kinase.
DR   PANTHER; PTHR10048; PTHR10048; 1.
DR   Pfam; PF00454; PI3_PI4_kinase; 1.
DR   Pfam; PF00613; PI3Ka; 1.
DR   Pfam; PF19274; PI4K_N; 1.
DR   SMART; SM00145; PI3Ka; 1.
DR   SMART; SM00146; PI3Kc; 1.
DR   SUPFAM; SSF48371; SSF48371; 2.
DR   SUPFAM; SSF56112; SSF56112; 1.
DR   PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR   PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR   PROSITE; PS50290; PI3_4_KINASE_3; 1.
DR   PROSITE; PS51545; PIK_HELICAL; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Alternative splicing; ATP-binding; Cell membrane; Cytoplasm;
KW   Disease variant; Hereditary spastic paraplegia; Kinase; Lipid metabolism;
KW   Membrane; Neurodegeneration; Nucleotide-binding; Phosphoprotein;
KW   Reference proteome; Transferase.
FT   CHAIN           1..2102
FT                   /note="Phosphatidylinositol 4-kinase alpha"
FT                   /id="PRO_0000088827"
FT   DOMAIN          1530..1718
FT                   /note="PIK helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00878"
FT   DOMAIN          1808..2086
FT                   /note="PI3K/PI4K catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   REGION          1814..1820
FT                   /note="G-loop"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   REGION          1954..1962
FT                   /note="Catalytic loop"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   REGION          1973..1997
FT                   /note="Activation loop"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00269"
FT   MOD_RES         230
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         256
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         257
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648,
FT                   ECO:0007744|PubMed:23186163"
FT   MOD_RES         259
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:E9Q3L2"
FT   MOD_RES         260
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648"
FT   MOD_RES         262
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648,
FT                   ECO:0007744|PubMed:23186163"
FT   MOD_RES         265
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:18669648,
FT                   ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19690332,
FT                   ECO:0007744|PubMed:23186163"
FT   MOD_RES         429
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         1154
FT                   /note="Phosphotyrosine"
FT                   /evidence="ECO:0007744|PubMed:23186163"
FT   MOD_RES         1436
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19690332,
FT                   ECO:0007744|PubMed:23186163, ECO:0007744|PubMed:24275569"
FT   VAR_SEQ         1..1248
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:7961848"
FT                   /id="VSP_008805"
FT   VARIANT         119
FT                   /note="R -> W (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086468"
FT   VARIANT         380
FT                   /note="M -> V (in dbSNP:rs17819211)"
FT                   /id="VAR_050531"
FT   VARIANT         472
FT                   /note="S -> R (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086469"
FT   VARIANT         566..2102
FT                   /note="Missing (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086470"
FT   VARIANT         618..2102
FT                   /note="Missing (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086471"
FT   VARIANT         777
FT                   /note="L -> P (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086472"
FT   VARIANT         796..2102
FT                   /note="Missing (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:25855803"
FT                   /id="VAR_086473"
FT   VARIANT         1152
FT                   /note="E -> K (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086474"
FT   VARIANT         1191..2102
FT                   /note="Missing (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086475"
FT   VARIANT         1198
FT                   /note="A -> T (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086476"
FT   VARIANT         1295
FT                   /note="H -> R (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086477"
FT   VARIANT         1556
FT                   /note="V -> M (in SPG84; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086478"
FT   VARIANT         1623
FT                   /note="Y -> D (in GIDID2; decreased interaction with TTC7A
FT                   resulting in reduced PI4K complex stability; no effect on
FT                   kinase activity)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086479"
FT   VARIANT         1664
FT                   /note="D -> N (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086480"
FT   VARIANT         1720
FT                   /note="T -> I (in SPG84; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086481"
FT   VARIANT         1733
FT                   /note="R -> W (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086482"
FT   VARIANT         1808
FT                   /note="K -> T (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086483"
FT   VARIANT         1820
FT                   /note="Missing (in SPG84; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086484"
FT   VARIANT         1851
FT                   /note="V -> L (in dbSNP:rs2539908)"
FT                   /id="VAR_059549"
FT   VARIANT         1854
FT                   /note="D -> N (in NEDSPLB; loss of kinase activity; no
FT                   effect on protein abundance; dbSNP:rs747119727)"
FT                   /evidence="ECO:0000269|PubMed:25855803,
FT                   ECO:0000269|PubMed:34415310, ECO:0000269|PubMed:34415322"
FT                   /id="VAR_074640"
FT   VARIANT         1925
FT                   /note="G -> E (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086485"
FT   VARIANT         1925
FT                   /note="G -> R (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086486"
FT   VARIANT         1937
FT                   /note="Y -> C (in NEDSPLB)"
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT                   /id="VAR_086487"
FT   VARIANT         1987
FT                   /note="N -> S (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086488"
FT   VARIANT         2041
FT                   /note="M -> T (in NEDSPLB; unknown pathological
FT                   significance)"
FT                   /evidence="ECO:0000269|PubMed:34415322"
FT                   /id="VAR_086489"
FT   MUTAGEN         1957
FT                   /note="D->A: Loss of kinase activity."
FT                   /evidence="ECO:0000269|PubMed:34415310"
FT   CONFLICT        438
FT                   /note="N -> S (in Ref. 3; AAD13352)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        1947
FT                   /note="S -> I (in Ref. 4; AAH53654)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   2102 AA;  236830 MW;  ED4C3C9EA79E6B5D CRC64;
     MAAAPARGGG GGGGGGGGCS GSGSSASRGF YFNTVLSLAR SLAVQRPASL EKVQKLLCMC
     PVDFHGIFQL DERRRDAVIA LGIFLIESDL QHKDCVVPYL LRLLKGLPKV YWVEESTARK
     GRGALPVAES FSFCLVTLLS DVAYRDPSLR DEILEVLLQV LHVLLGMCQA LEIQDKEYLC
     KYAIPCLIGI SRAFGRYSNM EESLLSKLFP KIPPHSLRVL EELEGVRRRS FNDFRSILPS
     NLLTVCQEGT LKRKTSSVSS ISQVSPERGM PPPSSPGGSA FHYFEASCLP DGTALEPEYY
     FSTISSSFSV SPLFNGVTYK EFNIPLEMLR ELLNLVKKIV EEAVLKSLDA IVASVMEANP
     SADLYYTSFS DPLYLTMFKM LRDTLYYMKD LPTSFVKEIH DFVLEQFNTS QGELQKILHD
     ADRIHNELSP LKLRCQANAA CVDLMVWAVK DEQGAENLCI KLSEKLQSKT SSKVIIAHLP
     LLICCLQGLG RLCERFPVVV HSVTPSLRDF LVIPSPVLVK LYKYHSQYHT VAGNDIKISV
     TNEHSESTLN VMSGKKSQPS MYEQLRDIAI DNICRCLKAG LTVDPVIVEA FLASLSNRLY
     ISQESDKDAH LIPDHTIRAL GHIAVALRDT PKVMEPILQI LQQKFCQPPS PLDVLIIDQL
     GCLVITGNQY IYQEVWNLFQ QISVKASSVV YSATKDYKDH GYRHCSLAVI NALANIAANI
     QDEHLVDELL MNLLELFVQL GLEGKRASER ASEKGPALKA SSSAGNLGVL IPVIAVLTRR
     LPPIKEAKPR LQKLFRDFWL YSVLMGFAVE GSGLWPEEWY EGVCEIATKS PLLTFPSKEP
     LRSVLQYNSA MKNDTVTPAE LSELRSTIIN LLDPPPEVSA LINKLDFAMS TYLLSVYRLE
     YMRVLRSTDP DRFQVMFCYF EDKAIQKDKS GMMQCVIAVA DKVFDAFLNM MADKAKTKEN
     EEELERHAQF LLVNFNHIHK RIRRVADKYL SGLVDKFPHL LWSGTVLKTM LDILQTLSLS
     LSADIHKDQP YYDIPDAPYR ITVPDTYEAR ESIVKDFAAR CGMILQEAMK WAPTVTKSHL
     QEYLNKHQNW VSGLSQHTGL AMATESILHF AGYNKQNTTL GATQLSERPA CVKKDYSNFM
     ASLNLRNRYA GEVYGMIRFS GTTGQMSDLN KMMVQDLHSA LDRSHPQHYT QAMFKLTAML
     ISSKDCDPQL LHHLCWGPLR MFNEHGMETA LACWEWLLAG KDGVEVPFMR EMAGAWHMTV
     EQKFGLFSAE IKEADPLAAS EASQPKPCPP EVTPHYIWID FLVQRFEIAK YCSSDQVEIF
     SSLLQRSMSL NIGGAKGSMN RHVAAIGPRF KLLTLGLSLL HADVVPNATI RNVLREKIYS
     TAFDYFSCPP KFPTQGEKRL REDISIMIKF WTAMFSDKKY LTASQLVPPD NQDTRSNLDI
     TVGSRQQATQ GWINTYPLSS GMSTISKKSG MSKKTNRGSQ LHKYYMKRRT LLLSLLATEI
     ERLITWYNPL SAPELELDQA GENSVANWRS KYISLSEKQW KDNVNLAWSI SPYLAVQLPA
     RFKNTEAIGN EVTRLVRLDP GAVSDVPEAI KFLVTWHTID ADAPELSHVL CWAPTDPPTG
     LSYFSSMYPP HPLTAQYGVK VLRSFPPDAI LFYIPQIVQA LRYDKMGYVR EYILWAASKS
     QLLAHQFIWN MKTNIYLDEE GHQKDPDIGD LLDQLVEEIT GSLSGPAKDF YQREFDFFNK
     ITNVSAIIKP YPKGDERKKA CLSALSEVKV QPGCYLPSNP EAIVLDIDYK SGTPMQSAAK
     APYLAKFKVK RCGVSELEKE GLRCRSDSED ECSTQEADGQ KISWQAAIFK VGDDCRQDML
     ALQIIDLFKN IFQLVGLDLF VFPYRVVATA PGCGVIECIP DCTSRDQLGR QTDFGMYDYF
     TRQYGDESTL AFQQARYNFI RSMAAYSLLL FLLQIKDRHN GNIMLDKKGH IIHIDFGFMF
     ESSPGGNLGW EPDIKLTDEM VMIMGGKMEA TPFKWFMEMC VRGYLAVRPY MDAVVSLVTL
     MLDTGLPCFR GQTIKLLKHR FSPNMTEREA ANFIMKVIQS CFLSNRSRTY DMIQYYQNDI
     PY
 
 
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