PI5K4_ARATH
ID PI5K4_ARATH Reviewed; 779 AA.
AC Q9M1K2;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase 4;
DE Short=AtPIP5K4;
DE EC=2.7.1.68;
DE AltName: Full=1-phosphatidylinositol 4-phosphate kinase 4;
DE AltName: Full=Diphosphoinositide kinase 4;
DE AltName: Full=PtdIns(4)P-5-kinase 4;
GN Name=PIP5K4; OrderedLocusNames=At3g56960; ORFNames=F24I3.40;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12226484; DOI=10.1104/pp.004770;
RA Mueller-Roeber B., Pical C.;
RT "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT putative isoforms of inositol phospholipid kinase and phosphoinositide-
RT specific phospholipase C.";
RL Plant Physiol. 130:22-46(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-
CC phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
CC inositol-4,5-bisphosphate) + ADP + H(+); Xref=Rhea:RHEA:14425,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:58178,
CC ChEBI:CHEBI:58456, ChEBI:CHEBI:456216; EC=2.7.1.68;
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DR EMBL; AL138655; CAB72166.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79593.1; -; Genomic_DNA.
DR EMBL; CP002686; ANM63864.1; -; Genomic_DNA.
DR PIR; T47756; T47756.
DR RefSeq; NP_001319773.1; NM_001339834.1.
DR RefSeq; NP_191255.1; NM_115555.2.
DR AlphaFoldDB; Q9M1K2; -.
DR SMR; Q9M1K2; -.
DR BioGRID; 10179; 2.
DR STRING; 3702.AT3G56960.1; -.
DR iPTMnet; Q9M1K2; -.
DR PaxDb; Q9M1K2; -.
DR PRIDE; Q9M1K2; -.
DR ProteomicsDB; 236736; -.
DR EnsemblPlants; AT3G56960.1; AT3G56960.1; AT3G56960.
DR EnsemblPlants; AT3G56960.2; AT3G56960.2; AT3G56960.
DR GeneID; 824863; -.
DR Gramene; AT3G56960.1; AT3G56960.1; AT3G56960.
DR Gramene; AT3G56960.2; AT3G56960.2; AT3G56960.
DR KEGG; ath:AT3G56960; -.
DR Araport; AT3G56960; -.
DR TAIR; locus:2080585; AT3G56960.
DR eggNOG; KOG0229; Eukaryota.
DR HOGENOM; CLU_004312_6_4_1; -.
DR InParanoid; Q9M1K2; -.
DR OMA; EENLEWD; -.
DR OrthoDB; 259109at2759; -.
DR PhylomeDB; Q9M1K2; -.
DR BioCyc; ARA:AT3G56960-MON; -.
DR PRO; PR:Q9M1K2; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M1K2; baseline and differential.
DR Genevisible; Q9M1K2; AT.
DR GO; GO:0016324; C:apical plasma membrane; IDA:TAIR.
DR GO; GO:0005829; C:cytosol; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006897; P:endocytosis; IMP:TAIR.
DR GO; GO:0007164; P:establishment of tissue polarity; IMP:TAIR.
DR GO; GO:0046488; P:phosphatidylinositol metabolic process; IDA:TAIR.
DR GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0009827; P:plant-type cell wall modification; IMP:TAIR.
DR GO; GO:0009846; P:pollen germination; IGI:TAIR.
DR GO; GO:0009860; P:pollen tube growth; IMP:TAIR.
DR GO; GO:0010118; P:stomatal movement; IMP:TAIR.
DR Gene3D; 3.30.800.10; -; 1.
DR InterPro; IPR003409; MORN.
DR InterPro; IPR017163; PIno-4-P-5_kinase_pln.
DR InterPro; IPR023610; PInositol-4-P-5-kinase.
DR InterPro; IPR002498; PInositol-4-P-5-kinase_core.
DR InterPro; IPR027484; PInositol-4-P-5-kinase_N.
DR PANTHER; PTHR23086; PTHR23086; 1.
DR Pfam; PF02493; MORN; 8.
DR Pfam; PF01504; PIP5K; 1.
DR PIRSF; PIRSF037274; PIP5K_plant_prd; 1.
DR SMART; SM00698; MORN; 7.
DR SMART; SM00330; PIPKc; 1.
DR PROSITE; PS51455; PIPK; 1.
PE 4: Predicted;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Repeat;
KW Transferase.
FT CHAIN 1..779
FT /note="Phosphatidylinositol 4-phosphate 5-kinase 4"
FT /id="PRO_0000185476"
FT REPEAT 77..99
FT /note="MORN 1"
FT REPEAT 100..122
FT /note="MORN 2"
FT REPEAT 123..145
FT /note="MORN 3"
FT REPEAT 146..168
FT /note="MORN 4"
FT REPEAT 169..191
FT /note="MORN 5"
FT REPEAT 192..214
FT /note="MORN 6"
FT REPEAT 215..237
FT /note="MORN 7"
FT REPEAT 238..259
FT /note="MORN 8"
FT DOMAIN 382..775
FT /note="PIPK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00781"
FT REGION 20..61
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 735..756
FT /note="Activation loop"
FT /evidence="ECO:0000250"
FT COMPBIAS 21..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 779 AA; 89238 MW; FAEB3BF1603C18B0 CRC64;
MSKEQSCVLK AWEVTVRKTQ QAKKRANSIF GTVSVAPQTD DDATTTTEEN DDETSTNRSS
IGELYHAERI LPNGDYYTGQ WYDSFPHGHG KYLWTDGCMY IGDWYNGKTM GRGKFGWPSG
ATYEGEFKSG YMDGVGLYTG PSGDTYKGQW VMNLKHGHGI KRFANGDVYD GEWRRGLQEA
QGKYQWRDGS YYMGEWKNAT ICGKGTFIWT DGNRYDGFWD DGFPRGNGTF KWADGSFYVG
HWSNDPEEMN GTYYPSGDDG SPEWDPKDVF TNLSEYKICS GERVPVLPSQ KKLSVWNSSK
RVEKPRRMSV DGRVSVGVDR AFEKMNMWGT ESGEGAADID STTRRDLDAE MMRLEAEGFI
QSLRPSPAPM RLPRAGRKQG ETISKGHRNY ELMLNLQLGI RHAVGKQAPV VSLDLKHSAF
DPKEKVWTRF PPEGTKYTPP HQSSEFKWKD YCPLVFRSLR KLFKVDPADY MLSICGNDAL
RELSSPGKSG SFFYLTNDDR YMIKTMKKSE TKVLLRMLAA YYNHVRAFEN TLVIRFYGLH
CVKLTGPIQK KVRFVIMGNL FCSEYSIHRR FDLKGSSLGR TTDKPESEIN SNTILKDLDL
NFIFRLQKAW YQEFIRQVDK DCEFLEQERI MDYSLLVGIH FREASVAGEL IPSGARTPIG
EFEDESAPRL SRADVDQLLS DPTRWASIRL GGNMPARAER TMRRSDCEFQ LVGEPTGEYY
EVVMIFGIID ILQDYDISKK LEHAYKSIQY DPTSISAVDP RLYSRRFRDF IFKVFTEDN
