PI5K6_ARATH
ID PI5K6_ARATH Reviewed; 715 AA.
AC Q9SFB8;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase 6;
DE Short=AtPIP5K6;
DE EC=2.7.1.68;
DE AltName: Full=1-phosphatidylinositol 4-phosphate kinase 6;
DE AltName: Full=Diphosphoinositide kinase 6;
DE AltName: Full=PtdIns(4)P-5-kinase 6;
GN Name=PIP5K6; OrderedLocusNames=At3g07960; ORFNames=F17A17.30;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12226484; DOI=10.1104/pp.004770;
RA Mueller-Roeber B., Pical C.;
RT "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT putative isoforms of inositol phospholipid kinase and phosphoinositide-
RT specific phospholipase C.";
RL Plant Physiol. 130:22-46(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-
CC phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
CC inositol-4,5-bisphosphate) + ADP + H(+); Xref=Rhea:RHEA:14425,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:58178,
CC ChEBI:CHEBI:58456, ChEBI:CHEBI:456216; EC=2.7.1.68;
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DR EMBL; AC013483; AAF21206.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74625.1; -; Genomic_DNA.
DR EMBL; BT008595; AAP40421.1; -; mRNA.
DR RefSeq; NP_187453.1; NM_111675.6.
DR AlphaFoldDB; Q9SFB8; -.
DR SMR; Q9SFB8; -.
DR BioGRID; 5322; 1.
DR STRING; 3702.AT3G07960.1; -.
DR iPTMnet; Q9SFB8; -.
DR PaxDb; Q9SFB8; -.
DR PRIDE; Q9SFB8; -.
DR ProteomicsDB; 234955; -.
DR EnsemblPlants; AT3G07960.1; AT3G07960.1; AT3G07960.
DR GeneID; 819987; -.
DR Gramene; AT3G07960.1; AT3G07960.1; AT3G07960.
DR KEGG; ath:AT3G07960; -.
DR Araport; AT3G07960; -.
DR TAIR; locus:2077397; AT3G07960.
DR eggNOG; KOG0229; Eukaryota.
DR HOGENOM; CLU_004312_6_4_1; -.
DR InParanoid; Q9SFB8; -.
DR OrthoDB; 229459at2759; -.
DR PhylomeDB; Q9SFB8; -.
DR BioCyc; ARA:AT3G07960-MON; -.
DR PRO; PR:Q9SFB8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SFB8; baseline and differential.
DR Genevisible; Q9SFB8; AT.
DR GO; GO:0016324; C:apical plasma membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0090406; C:pollen tube; IDA:TAIR.
DR GO; GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IMP:TAIR.
DR GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.800.10; -; 1.
DR InterPro; IPR003409; MORN.
DR InterPro; IPR017163; PIno-4-P-5_kinase_pln.
DR InterPro; IPR023610; PInositol-4-P-5-kinase.
DR InterPro; IPR002498; PInositol-4-P-5-kinase_core.
DR InterPro; IPR027484; PInositol-4-P-5-kinase_N.
DR PANTHER; PTHR23086; PTHR23086; 1.
DR Pfam; PF02493; MORN; 7.
DR Pfam; PF01504; PIP5K; 1.
DR PIRSF; PIRSF037274; PIP5K_plant_prd; 1.
DR SMART; SM00698; MORN; 7.
DR SMART; SM00330; PIPKc; 1.
DR PROSITE; PS51455; PIPK; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Repeat;
KW Transferase.
FT CHAIN 1..715
FT /note="Phosphatidylinositol 4-phosphate 5-kinase 6"
FT /id="PRO_0000185478"
FT REPEAT 32..54
FT /note="MORN 1"
FT REPEAT 55..77
FT /note="MORN 2"
FT REPEAT 78..100
FT /note="MORN 3"
FT REPEAT 101..123
FT /note="MORN 4"
FT REPEAT 124..146
FT /note="MORN 5"
FT REPEAT 147..169
FT /note="MORN 6"
FT REPEAT 170..192
FT /note="MORN 7"
FT REPEAT 193..214
FT /note="MORN 8"
FT DOMAIN 321..711
FT /note="PIPK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00781"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 253..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 671..692
FT /note="Activation loop"
FT /evidence="ECO:0000250"
FT COMPBIAS 265..280
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..306
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 715 AA; 81422 MW; 795D86CEB1FB52E9 CRC64;
MSVAHADDAD DYSRPTGESY HAEKALPSGD FYTGQWRDNL PHGHGKYLWT DGCMYVGDWH
RGKTMGKGRF SWPSGATYEG DFKNGYMDGK GTYIDSSGDL YRGSWVMNLR HGQGTKSYVN
GDCYDGEWRR GLQDGHGRYQ WKNENHYIGQ WKNGLMNGNG TMIWSNGNRY DGSWEDGAPK
GNGTFRWSDG SFYVGVWSKD PKEQNGTYYP STSSGNFDWQ PQQVFYVDLS ECVVCTCQRI
PVLPSQKMPV WYGASEQSSS GNRTKNSERP RRRSVDGRVS NGEMELRSNG SGYLQVDDNA
ESTRSSLGPL RIQPAKKQGQ TISKGHKNYE LMLNLQLGIR HSVGRPAPAT SLDLKASAFD
PKEKLWTKFP SEGSKYTPPH QSCEFKWKDY CPVVFRTLRK LFSVDAADYM LSICGNDALR
ELSSPGKSGS FFYLTNDDRY MIKTMKKAET KVLIRMLPAY YNHVRACENT LVTKFFGLHC
VKLTGTAQKK VRFVIMGNLF CTGHSIHRRF DLKGSSHGRL TTKPESEIDP NTTLKDLDLN
FAFRLQKNWF QEFCRQVDRD CEFLEQERIM DYSLLVGLHF REAAIKDSAT PTSGARTPTG
NSETRLSRAE MDRFLLDASK LASIKLGINM PARVERTARR SDCENQLVGD PTGEFYDVIV
YFGIIDILQD YDISKKLEHA YKSMQYDPTS ISAVDPKQYS RRFRDFIFRV FVEDA
