PI5KA_ARATH
ID PI5KA_ARATH Reviewed; 427 AA.
AC Q9LMN1; O23705;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 25-OCT-2005, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Phosphatidylinositol 4-phosphate 5-kinase 10;
DE Short=AtPIP5K10;
DE EC=2.7.1.68;
DE AltName: Full=1-phosphatidylinositol 4-phosphate kinase 10;
DE AltName: Full=Diphosphoinositide kinase 10;
DE AltName: Full=PtdIns(4)P-5-kinase 10;
GN Name=PIP5K10; OrderedLocusNames=At1g01460; ORFNames=F22L4.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9666060; DOI=10.1016/s0378-1119(98)00286-8;
RA Terryn N., Gielen J., De Keyser A., Van Den Daele H., Ardiles W., Neyt P.,
RA De Clercq R., Coppieters J., Dehais P., Villarroel R., Rouze P.,
RA van Montagu M.;
RT "Sequence analysis of a 40-kb Arabidopsis thaliana genomic region located
RT at the top of chromosome 1.";
RL Gene 215:11-17(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12226484; DOI=10.1104/pp.004770;
RA Mueller-Roeber B., Pical C.;
RT "Inositol phospholipid metabolism in Arabidopsis. Characterized and
RT putative isoforms of inositol phospholipid kinase and phosphoinositide-
RT specific phospholipase C.";
RL Plant Physiol. 130:22-46(2002).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-
CC phosphate) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
CC inositol-4,5-bisphosphate) + ADP + H(+); Xref=Rhea:RHEA:14425,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:58178,
CC ChEBI:CHEBI:58456, ChEBI:CHEBI:456216; EC=2.7.1.68;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF81306.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Y12776; CAA73312.1; -; Genomic_DNA.
DR EMBL; AC061957; AAF81306.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE27290.1; -; Genomic_DNA.
DR EMBL; DQ056443; AAY78600.1; -; mRNA.
DR PIR; C86145; C86145.
DR RefSeq; NP_171653.2; NM_100028.3.
DR AlphaFoldDB; Q9LMN1; -.
DR SMR; Q9LMN1; -.
DR STRING; 3702.AT1G01460.1; -.
DR PaxDb; Q9LMN1; -.
DR PRIDE; Q9LMN1; -.
DR ProteomicsDB; 234755; -.
DR EnsemblPlants; AT1G01460.1; AT1G01460.1; AT1G01460.
DR GeneID; 839469; -.
DR Gramene; AT1G01460.1; AT1G01460.1; AT1G01460.
DR KEGG; ath:AT1G01460; -.
DR Araport; AT1G01460; -.
DR TAIR; locus:2025336; AT1G01460.
DR eggNOG; KOG0229; Eukaryota.
DR HOGENOM; CLU_004312_6_3_1; -.
DR InParanoid; Q9LMN1; -.
DR OMA; MKSHENA; -.
DR OrthoDB; 1562683at2759; -.
DR PhylomeDB; Q9LMN1; -.
DR BioCyc; ARA:AT1G01460-MON; -.
DR BRENDA; 2.7.1.68; 399.
DR PRO; PR:Q9LMN1; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9LMN1; baseline and differential.
DR Genevisible; Q9LMN1; AT.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046854; P:phosphatidylinositol phosphate biosynthetic process; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR Gene3D; 3.30.800.10; -; 1.
DR InterPro; IPR023610; PInositol-4-P-5-kinase.
DR InterPro; IPR002498; PInositol-4-P-5-kinase_core.
DR InterPro; IPR027484; PInositol-4-P-5-kinase_N.
DR PANTHER; PTHR23086; PTHR23086; 1.
DR Pfam; PF01504; PIP5K; 1.
DR SMART; SM00330; PIPKc; 1.
DR PROSITE; PS51455; PIPK; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Kinase; Nucleotide-binding; Reference proteome; Transferase.
FT CHAIN 1..427
FT /note="Phosphatidylinositol 4-phosphate 5-kinase 10"
FT /id="PRO_0000185482"
FT DOMAIN 1..419
FT /note="PIPK"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00781"
FT REGION 247..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 334..355
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 379..400
FT /note="Activation loop"
FT /evidence="ECO:0000250"
SQ SEQUENCE 427 AA; 48549 MW; 87EDC9019FAFC28F CRC64;
MFTREITAKD VKATEKNRIR YSSKHIKHLP PGTITEFEWK DYCPLGFRLI QELEDINHDE
YMKSICNDET LRKLSTSKVG NMFLLSKDDR FLIKILRKSE IKVILEMLPG YFRHIHKYRS
TLLSKNYGAH SVKPIGGVKT YFVVMSNILQ SDVFMNKVYD LKGSSQGRTN KKIKVRDKTI
LKDIDLDFCF YVDSLARHRL IKQTKLDCEL LEDEGIMDYS LMLGLQVKGS CHGSIDELIP
VYDSFTSRGS VDSNSSKFMK TASNSPDRSS STMYSCTPSR NSVDSENSVN IQSVASISPS
PAQTNASDSP YESLVSKTNL TNIFQNSSST NFGMKIPGRA RRVGRGESGS VVGKQSREGG
EEWYDVILYL GIIDIFQDYG VRKRLEHCYK SIQHSSKTIS AVHPKMYSSR FQDFVSQIFL
PDEDPSH
