PICO_DROME
ID PICO_DROME Reviewed; 529 AA.
AC Q9V7S5; O61364; Q8T077; Q9V7S6;
DT 28-MAR-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Putative inorganic phosphate cotransporter;
GN Name=Picot; ORFNames=CG8098;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 449-529.
RC STRAIN=Canton-S;
RA Da Lage J.-L.;
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be an inorganic phosphate cotransporter.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=Q9V7S5-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q9V7S5-2; Sequence=VSP_007009;
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. Sodium/anion
CC cotransporter family. {ECO:0000305}.
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DR EMBL; AE013599; AAF57968.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF57969.1; -; Genomic_DNA.
DR EMBL; AY069501; AAL39646.1; -; mRNA.
DR EMBL; AF022713; AAD09148.1; -; Genomic_DNA.
DR RefSeq; NP_001286496.1; NM_001299567.1. [Q9V7S5-2]
DR RefSeq; NP_001286497.1; NM_001299568.1. [Q9V7S5-2]
DR RefSeq; NP_611146.1; NM_137302.3. [Q9V7S5-2]
DR RefSeq; NP_725600.1; NM_166187.2. [Q9V7S5-1]
DR AlphaFoldDB; Q9V7S5; -.
DR SMR; Q9V7S5; -.
DR BioGRID; 62577; 6.
DR DIP; DIP-22400N; -.
DR STRING; 7227.FBpp0086261; -.
DR TCDB; 2.A.1.14.34; the major facilitator superfamily (mfs).
DR SwissPalm; Q9V7S5; -.
DR PaxDb; Q9V7S5; -.
DR PRIDE; Q9V7S5; -.
DR DNASU; 36865; -.
DR EnsemblMetazoa; FBtr0087114; FBpp0086260; FBgn0024315. [Q9V7S5-2]
DR EnsemblMetazoa; FBtr0087115; FBpp0086261; FBgn0024315. [Q9V7S5-1]
DR EnsemblMetazoa; FBtr0340067; FBpp0309073; FBgn0024315. [Q9V7S5-2]
DR EnsemblMetazoa; FBtr0340068; FBpp0309074; FBgn0024315. [Q9V7S5-2]
DR GeneID; 36865; -.
DR KEGG; dme:Dmel_CG8098; -.
DR UCSC; CG8098-RA; d. melanogaster. [Q9V7S5-1]
DR CTD; 36865; -.
DR FlyBase; FBgn0024315; Picot.
DR VEuPathDB; VectorBase:FBgn0024315; -.
DR eggNOG; KOG2532; Eukaryota.
DR GeneTree; ENSGT00940000173740; -.
DR InParanoid; Q9V7S5; -.
DR OMA; MRFLGYG; -.
DR PhylomeDB; Q9V7S5; -.
DR BioGRID-ORCS; 36865; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36865; -.
DR PRO; PR:Q9V7S5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0024315; Expressed in adult hindgut (Drosophila) and 35 other tissues.
DR ExpressionAtlas; Q9V7S5; baseline and differential.
DR Genevisible; Q9V7S5; DM.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0015114; F:phosphate ion transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006820; P:anion transport; IBA:GO_Central.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR004745; Pi_cotranspt.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00894; 2A0114euk; 1.
DR PROSITE; PS50850; MFS; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..529
FT /note="Putative inorganic phosphate cotransporter"
FT /id="PRO_0000220946"
FT TRANSMEM 37..57
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 110..130
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 429..449
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 495..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 502..529
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..35
FT /note="MPFRRSSLNHRHRDGHVLVWNQRNLHESLEQQPQR -> MSASKEAICGSTE
FT KDLEKPALG (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_007009"
SQ SEQUENCE 529 AA; 58372 MW; 07B89A52D5081EFB CRC64;
MPFRRSSLNH RHRDGHVLVW NQRNLHESLE QQPQRCFATR YFVTFMLFLG MANAYVMRTN
MSVAIVAMVN HTAIKSGEAE EYDDECGDRD IPIDDSQDGE FAWSSALQGY ILSSFFYGYV
ITQIPFGILA KKYGSLRFLG YGMLINSVFA FLVPVAARGG GVWGLCAVRF IQGLGEGPIV
PCTHAMLAKW IPPNERSRMG AAVYAGAQFG TIISMPLSGL LAEYGFDGGW PSIFYVFGIV
GTVWSIAFLI FVHEDPSSHP TIDEREKKYI NDSLWGTDVV KSPPIPFKAI IKSLPFYAIL
FAHMGHNYGY ETLMTELPTY MKQVLRFSLK SNGLLSSLPY LAMWLFSMFI SVVADWMISS
KRFSHTATRK LINSIGQYGP GVALIAASYT GCDRALTLAI LTIGVGLNGG IYSGFKINHL
DLTPRFAGFL MSITNCSANL AGLLAPIAAG HLISDPSKPM MGQWQIVFFI AAFVYIICGT
FYNIFGSGER QYWDNPEDDE QKPALQTTVT TSPARLSNGS TAPAAISSS
