ID   PID4_CAEEL              Reviewed;         515 AA.
AC   O44749;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Protein pid-4 {ECO:0000305};
DE   AltName: Full=piRNA-induced silencing defective protein 4 {ECO:0000312|WormBase:W03G9.2};
GN   Name=pid-4 {ECO:0000312|WormBase:W03G9.2};
GN   ORFNames=W03G9.2 {ECO:0000312|WormBase:W03G9.2};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, INTERACTION WITH PID-2; APP-1 AND PRMT-5, SUBCELLULAR LOCATION,
RP   AND DEVELOPMENTAL STAGE.
RX   PubMed=33231880; DOI=10.15252/embj.2020105280;
RA   Placentino M., de Jesus Domingues A.M., Schreier J., Dietz S., Hellmann S.,
RA   de Albuquerque B.F., Butter F., Ketting R.F.;
RT   "Intrinsically disordered protein PID-2 modulates Z granules and is
RT   required for heritable piRNA-induced silencing in the Caenorhabditis
RT   elegans embryo.";
RL   EMBO J. 40:e105280-e105280(2021).
CC   -!- FUNCTION: Together with pid-5, it is involved in gene silencing
CC       mediated by a class of 21 nucleotide PIWI-interacting RNAs (piRNAs)
CC       that possess a uracil residue at the 5'-end (also called 21U-RNAs) and
CC       guide the Piwi protein prg-1 to its DNA targets for silencing
CC       (PubMed:33231880). Together with pid-5, it is required for the
CC       biogenesis of secondary and tertiary 22G-siRNAs (PubMed:33231880).
CC       Specifically, promotes the production of 22G-siRNAs from the 5' end of
CC       target mRNAs (PubMed:33231880). Together with pid-5, plays a role in
CC       small RNA-directed transgenerational epigenetic inheritance (also
CC       called RNAe) over several generations and germline immortality
CC       (PubMed:33231880). Together with pid-5, plays a role in the formation
CC       of liquid-like condensates in the cytoplasm called Z granules
CC       (PubMed:33231880). {ECO:0000269|PubMed:33231880}.
CC   -!- SUBUNIT: May interact with pid-2, app-1 and prmt-5.
CC       {ECO:0000269|PubMed:33231880}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, perinuclear region
CC       {ECO:0000269|PubMed:33231880}. Cytoplasm, P-body
CC       {ECO:0000269|PubMed:33231880}. Note=Co-localizes with pid-5 at P
CC       granules in germ cells. {ECO:0000269|PubMed:33231880}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in germ cells of L4 larvae
CC       (PubMed:33231880). Expressed at high levels in the pachytene stage of
CC       the meiotic region of L4 larvae (PubMed:33231880).
CC       {ECO:0000269|PubMed:33231880}.
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DR   EMBL; BX284601; CCD70126.1; -; Genomic_DNA.
DR   PIR; T32752; T32752.
DR   RefSeq; NP_491488.1; NM_059087.1.
DR   AlphaFoldDB; O44749; -.
DR   DIP; DIP-55471N; -.
DR   IntAct; O44749; 3.
DR   STRING; 6239.W03G9.2; -.
DR   EPD; O44749; -.
DR   PaxDb; O44749; -.
DR   PeptideAtlas; O44749; -.
DR   EnsemblMetazoa; W03G9.2.1; W03G9.2.1; WBGene00021014.
DR   GeneID; 189170; -.
DR   KEGG; cel:CELE_W03G9.2; -.
DR   UCSC; W03G9.2; c. elegans.
DR   CTD; 189170; -.
DR   WormBase; W03G9.2; CE14556; WBGene00021014; pid-4.
DR   eggNOG; ENOG502RTA2; Eukaryota.
DR   HOGENOM; CLU_026399_0_0_1; -.
DR   InParanoid; O44749; -.
DR   OMA; MFRRARH; -.
DR   OrthoDB; 1710253at2759; -.
DR   SignaLink; O44749; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00021014; Expressed in germ line (C elegans) and 3 other tissues.
DR   GO; GO:0000932; C:P-body; IEA:UniProtKB-SubCell.
DR   GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0031047; P:gene silencing by RNA; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Cytoplasm; Reference proteome; RNA-mediated gene silencing.
FT   CHAIN           1..515
FT                   /note="Protein pid-4"
FT                   /id="PRO_0000452725"
FT   REGION          496..515
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   515 AA;  59138 MW;  DFB6C19D4F17191B CRC64;
     MNTHNARDSD DESPLESEEH LKRFFAGRNL YSQTKIDDAR KKSHDAKYAK EVEWDAKDTA
     KGKPVFKKYR QEIADKTSRC DAQFINNLHT YYGNGSELRE LPETHYAVDF MGTTIATTEM
     TAELKKLKVL KKKTATSQFV REHRQLGFIP TPSRRSLSCT GRFMNFETKC DERPDRTNPE
     FSVGNNNFLS ATISNLDEDF KTPSNCEHVA SIVPKEISNA LTALIPHVNV FDYEKGEMEI
     GKVLCSNKNL HAYVIPKQHD QEQMLKDFSA RFLAWYNKLS HLQRDTFGTD KDPKRSGKQE
     DGKPMKPYFW RVNVILCLQE FQEEGTMFRR ARHVCGIRPH KSQPGLDLYI EIDTGSMRAL
     KTNYLTVRKL PQEFANVPTP ILEVRFEGAK TEEDVARRIA HIRKTGRAYI KSFDTGKRKI
     YQNSFFNGQP KDWSNIETAV LLPPKESSMS KLFCEDRDCM GLCCTDQWLE NAESSPGIST
     IPASWIADKN KKPFVDRSPQ KFKFPASGSY MKPAN