PIEZO_ARATH
ID PIEZO_ARATH Reviewed; 2462 AA.
AC F4IN58; Q9ZU83; Q9ZU84; Q9ZU85;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Piezo-type mechanosensitive ion channel homolog;
GN OrderedLocusNames=At2g48060/At2g48040/At2g48050;
GN ORFNames=T9J23.21/T9J23.19/T9J23.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-339 AND 502-707.
RC STRAIN=cv. Columbia;
RX PubMed=12481096; DOI=10.1104/pp.010207;
RA Xiao Y.-L., Malik M., Whitelaw C.A., Town C.D.;
RT "Cloning and sequencing of cDNAs for hypothetical genes from chromosome 2
RT of Arabidopsis.";
RL Plant Physiol. 130:2118-2128(2002).
CC -!- FUNCTION: Pore-forming subunit of a mechanosensitive non-specific
CC cation channel, that conducts both sodium and potassium ions.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- MISCELLANEOUS: Piezo comes from the Greek 'piesi' meaning pressure.
CC -!- SIMILARITY: Belongs to the PIEZO (TC 1.A.75) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD13708.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to three different genes At2g48040, At2g48050 and At2g48060.; Evidence={ECO:0000305};
CC Sequence=AAD13709.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to three different genes At2g48040, At2g48050 and At2g48060.; Evidence={ECO:0000305};
CC Sequence=AAD13714.1; Type=Erroneous gene model prediction; Note=Was originally thought to correspond to three different genes At2g48040, At2g48050 and At2g48060.; Evidence={ECO:0000305};
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DR EMBL; AC006072; AAD13708.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC006072; AAD13709.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC006072; AAD13714.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10927.1; -; Genomic_DNA.
DR EMBL; AY461629; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AY461630; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; F84922; F84922.
DR PIR; G84922; G84922.
DR PIR; H84922; H84922.
DR RefSeq; NP_182327.6; NM_130373.6.
DR AlphaFoldDB; F4IN58; -.
DR STRING; 3702.AT2G48060.1; -.
DR TCDB; 1.A.75.1.4; the mechanical nociceptor, piezo (piezo) family.
DR iPTMnet; F4IN58; -.
DR PaxDb; F4IN58; -.
DR PRIDE; F4IN58; -.
DR EnsemblPlants; AT2G48060.1; AT2G48060.1; AT2G48060.
DR GeneID; 819418; -.
DR Gramene; AT2G48060.1; AT2G48060.1; AT2G48060.
DR KEGG; ath:AT2G48060; -.
DR Araport; AT2G48060; -.
DR TAIR; locus:2066367; AT2G48060.
DR eggNOG; KOG1893; Eukaryota.
DR HOGENOM; CLU_000444_0_0_1; -.
DR InParanoid; F4IN58; -.
DR PRO; PR:F4IN58; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IN58; baseline and differential.
DR Genevisible; F4IN58; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IEA:InterPro.
DR GO; GO:0050982; P:detection of mechanical stimulus; IMP:TAIR.
DR InterPro; IPR027272; Piezo.
DR InterPro; IPR031334; Piezo_RRas-bd_dom.
DR PANTHER; PTHR13167; PTHR13167; 1.
DR Pfam; PF12166; Piezo_RRas_bdg; 1.
PE 2: Evidence at transcript level;
KW Coiled coil; Ion channel; Ion transport; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..2462
FT /note="Piezo-type mechanosensitive ion channel homolog"
FT /id="PRO_0000430288"
FT TRANSMEM 5..25
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..77
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 347..367
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 404..424
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 467..487
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TRANSMEM 502..522
FT /note="Helical; Name=13"
FT /evidence="ECO:0000255"
FT TRANSMEM 554..574
FT /note="Helical; Name=14"
FT /evidence="ECO:0000255"
FT TRANSMEM 653..673
FT /note="Helical; Name=15"
FT /evidence="ECO:0000255"
FT TRANSMEM 694..714
FT /note="Helical; Name=16"
FT /evidence="ECO:0000255"
FT TRANSMEM 730..750
FT /note="Helical; Name=17"
FT /evidence="ECO:0000255"
FT TRANSMEM 792..812
FT /note="Helical; Name=18"
FT /evidence="ECO:0000255"
FT TRANSMEM 826..846
FT /note="Helical; Name=19"
FT /evidence="ECO:0000255"
FT TRANSMEM 1027..1047
FT /note="Helical; Name=20"
FT /evidence="ECO:0000255"
FT TRANSMEM 1050..1070
FT /note="Helical; Name=21"
FT /evidence="ECO:0000255"
FT TRANSMEM 1078..1098
FT /note="Helical; Name=22"
FT /evidence="ECO:0000255"
FT TRANSMEM 1143..1160
FT /note="Helical; Name=23"
FT /evidence="ECO:0000255"
FT TRANSMEM 1204..1224
FT /note="Helical; Name=24"
FT /evidence="ECO:0000255"
FT TRANSMEM 1228..1248
FT /note="Helical; Name=25"
FT /evidence="ECO:0000255"
FT TRANSMEM 1260..1280
FT /note="Helical; Name=26"
FT /evidence="ECO:0000255"
FT TRANSMEM 1310..1330
FT /note="Helical; Name=27"
FT /evidence="ECO:0000255"
FT TRANSMEM 1611..1631
FT /note="Helical; Name=28"
FT /evidence="ECO:0000255"
FT TRANSMEM 1647..1667
FT /note="Helical; Name=29"
FT /evidence="ECO:0000255"
FT TRANSMEM 1916..1936
FT /note="Helical; Name=30"
FT /evidence="ECO:0000255"
FT TRANSMEM 1956..1976
FT /note="Helical; Name=31"
FT /evidence="ECO:0000255"
FT TRANSMEM 1984..2004
FT /note="Helical; Name=32"
FT /evidence="ECO:0000255"
FT TRANSMEM 2012..2032
FT /note="Helical; Name=33"
FT /evidence="ECO:0000255"
FT TRANSMEM 2130..2150
FT /note="Helical; Name=34"
FT /evidence="ECO:0000255"
FT TRANSMEM 2369..2389
FT /note="Helical; Name=35"
FT /evidence="ECO:0000255"
FT REGION 927..947
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1543..1583
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 1347..1400
FT /evidence="ECO:0000255"
FT COMPBIAS 1556..1572
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 22
FT /note="W -> R (in Ref. 3; AY461629)"
FT /evidence="ECO:0000305"
FT CONFLICT 318
FT /note="F -> V (in Ref. 3; AY461629)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2462 AA; 282275 MW; 46555B2B7A7512F0 CRC64;
MASFLVGFLL PSLLLAAALI NWSVISFLDL IAFLLVHYIA PEIGYRFQRR HWLLWPIFIF
SFAVFLAQVV YLVIWAALGQ DWDTPDTGWM RVIGFMILKS WRNPTVMYFL ALQLLTSLVA
LADIYSSRFG FARWRDTWWS HFSGIFEHLG SHLRVASCLL LPAVQLAVGI CNPSWVSLPF
FIGSCAGLVD WSLTSNVSGL FRWWRVLYIY AGFNIVLLYL YQLPINFSDM IRWIASFIGL
FRISLETEGP DICSGLFLVL FYIMLSYVRS DLEDMDFIMS TSENNLAERL LPPKYSFFIR
ESRAGVRHTN VLLRGAVFKT FSINFFTYGF PVSLFALSFW SFHFASLCAF GLLAYVGYII
YAFPSLFQLH RLNGLLLVFI LLWAVSTYIF NVAFSFLNTK VGKFGLGMLV ALGNLVNNSV
FLYLSEESSR SSNERSYVEA DEETKVLVVA TIAWGLRKCS RAIMLALIFL IAMKPGFFHA
VYVIFFLMYL LSHNINRKIR KSLILLCEVH FALLYILEID LVSNSLKQEG SASREVLFQL
GLLRSESSWD FLEIALLACF CAIHNHGFEV LFSFSAIVRH TPSPPIGFSI LKAGLNKSVL
LSVYSSPSSS YSQDNTTYER HIASFLSAIG QKFLSMYRSC GTYIAFITIL ISVYLVKPNY
VSFGYIFLLL LWITGRQLFE ETKRRLWFPL KAYAVLVFMF IYCLSSFVSL QLWLSGFIDL
YFYLGYNSKA PLLDNVWESL AVLIVMQLYS YERRQSGHYI PGQSSLLHPG VFGFFERFLA
WHGQKILFAA LFYASLSPIS VFGFVYLLGL VICTTFPKSS SIPSKSFLIY TGFLVSAEYL
FQLWGMQAQM FPGQKYAELS FYLGLRVYEP GFWGIESGLR GKVLVVAACT LQYNVFRWLE
RTSGLTVIKG KYEEPCPLFV SAEDTTASVS SSNGENPSST DHASISMKQG EATSNSWPFF
SPRGNQGAGF LHPKTGGSES GSSRKFSFGH FWGSIKESHR WNRRRILALK KERFETQKNL
LKIYLKFWIE NMFNLYGLEI NMIALLLASF ALLNAISMVY IALLAACVLL RRRVIQKLWP
VVVFLFASIL AIEYVATWNS FLPSDQAPSE TSVHCHDCWS IAALYFKFCR ECWLGVRVDD
PRTLISYFVV FMLACFKLRA DHISSFSESS TYHQMKSQRK NSFVWRDLSF ETKSMWTVLD
YLRLYCYVHL LDVVLILILI TGTLEYDILH LGYLAFALVF ARMRLEILKK KNKIFRFLRV
YNFVLIIFSL AYQSPFVGNF NDGKCETVDY IYEVIGFYKY DYGFRITARS ALVEIIIFML
VSLQSYMFSS QEFDYVSRYL EAEQIGAIVR EQEKKAARKT EQLQQIREAE EKKRQRNLQV
EKMKSEMLNL RVQLHRMNSD SNFGVASPRT EGLRRRKSPY LIPDSGAASP EIDGVVHRKE
EQPIDEDSQY PFEAHEFPVS TTPEALDSPE YSFGASPCEI TEVQQDLDVM SMERERKQKS
EGKENPLISA VQLIGDGVSQ VQFIGNQAVN NLVNFLNISP ENSDTNEQSS VDDEVYDEME
SQKRKHTPFE RSTSLQSDRS SDGTSFQIGR IFRHIWSRMQ SNNDIVCYCC FIIAFLWNFS
LLSMVYLAAL FLYALCVHTG PTHIFWVIML MYTEIYILLQ YLYQIIIQHC GLSIDAPLLH
ELGFPTQRIK SSFVVSSLPL FLIYIFTLIQ SSITVKDGDW VPSADFTSRR NARGSQKDLT
RIRLSQRILD VFKKLRDSAK LVIRSIYRYW ISLTRGAESP PYFVQVTMDV HMWPEDGIQP
ERVECRMNQL LRLVHNERCE KGNPDLCPYS SRVHVQSIER STETPNEALV VLEVEYASPT
NGCSSAEWYK SLTPASDVAK EIRKAQHSGL GEGTGFPYPI LSVIGGGKRD TDLYAYIFGA
DLIVFFLVAI FYQSVIKNKS EFIDVYQLED QFPFDFVIIL MVIFFLIVVD RVIYLCSFAT
GKVVYYLFSL ILFTYAVTEY AWSIYPTQQH AAGLALRIIF LAKAMSLALQ AIQIRYGLPH
KSTLYRQFLT SEVSRINYYG YRLYRALPFL YELRCVLDWS CTATSLTMYD WLKLEDVNAS
LYLVKCDTVL NRATHKHGEK QTKMTKCCNG ICLFFILLCV IWAPMLMYSS GNPTNIANPI
KDASVQIDLK TVGGKLTLYQ TTLCERISGD NIDLGLDLGS QSFLPTYNKN DIQLICCQAD
ASVLWLVPDT VVTRFIQSLD WDTDMDITFT WVLNRDRPKG KETVKYERSV DPLDLPKRSD
IQMVLNGSMD GFRVHNLYPK FFRVTGSGDV RSFEDQTDEV SADILINHAN FKWWWSFHNL
KASENISACE GMDGPVAIIM SEETPPQGFL GDTLSKFSIW GLYITFVLAV GRFIRLQCSD
LRMRIPYENL PSCDRLIAIC EDLYAARAEG ELGVEEVLYW TLVKIYRSPH MLLEYTKLDY
DA
