PIEZO_DROME
ID PIEZO_DROME Reviewed; 2551 AA.
AC M9MSG8; H6X2P4;
DT 01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 1.
DT 03-AUG-2022, entry version 59.
DE RecName: Full=Piezo-type mechanosensitive ion channel component {ECO:0000305};
GN Name=Piezo {ECO:0000312|FlyBase:FBgn0264953};
GN ORFNames=CG18103 {ECO:0000312|FlyBase:FBgn0264953};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:AFB77909.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=22343900; DOI=10.1038/nature10812;
RA Coste B., Xiao B., Santos J.S., Syeda R., Grandl J., Spencer K.S.,
RA Kim S.E., Schmidt M., Mathur J., Dubin A.E., Montal M., Patapoutian A.;
RT "Piezo proteins are pore-forming subunits of mechanically activated
RT channels.";
RL Nature 483:176-181(2012).
RN [2] {ECO:0000312|EMBL:ADV36969.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|EMBL:ADV36969.1, ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305}
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22343891; DOI=10.1038/nature10801;
RA Kim S.E., Coste B., Chadha A., Cook B., Patapoutian A.;
RT "The role of Drosophila Piezo in mechanical nociception.";
RL Nature 483:209-212(2012).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=32649914; DOI=10.1016/j.cub.2020.05.076;
RA Zhang L., Yu J., Guo X., Wei J., Liu T., Zhang W.;
RT "Parallel Mechanosensory Pathways Direct Oviposition Decision-Making in
RT Drosophila.";
RL Curr. Biol. 30:3075-3088(2020).
CC -!- FUNCTION: Component of a mechanosensitive channel required for rapidly
CC adapting mechanically activated (MA) currents (PubMed:22343900,
CC PubMed:22343891). Plays a major role in nociception (response to strong
CC or painful touch) (PubMed:22343891). Required for maintaining the
CC mechanosensitivity of tarsal bristle mechanosensors (PubMed:32649914).
CC During their evalulation of potential egg-laying sites, females
CC determine the softest substrate for their eggs first by making a coarse
CC evaluation of substrate hardness using mechanosensitive channels nan
CC and Piezo in the leg tarsal bristles, followed by a much finer
CC assessment using nan, iav and Tmc mechanosensitive channels on the
CC labellum (PubMed:32649914). Acts in the nompC- and nan-expressing
CC neurons of the female leg tarsals, to sense the mild differences in
CC egg-laying substrate stiffness (PubMed:32649914).
CC {ECO:0000269|PubMed:22343891, ECO:0000269|PubMed:22343900,
CC ECO:0000269|PubMed:32649914}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22343900};
CC Multi-pass membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Viable and fertile, with no apparent coordination
CC defects (PubMed:22343891). Flies have significant defects in detection
CC of strong touch stimuli, however detection of gentle touch and
CC temperature is unaffected (PubMed:22343891). Decreases firing
CC frequencies of adult leg tarsal bristles (PubMed:32649914). In females,
CC no effect on texture selection during egg-laying (PubMed:32649914).
CC RNAi-mediated knockdown in NompC-expressing neurons, reduces the
CC female's preference for softer egg-laying substrates (PubMed:32649914).
CC {ECO:0000269|PubMed:22343891, ECO:0000269|PubMed:32649914}.
CC -!- MISCELLANEOUS: Piezo comes from the Greek 'piesi' meaning pressure.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PIEZO (TC 1.A.75) family. {ECO:0000305}.
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DR EMBL; JQ425255; AFB77909.1; -; mRNA.
DR EMBL; AE014134; ADV36969.1; -; Genomic_DNA.
DR RefSeq; NP_001188719.1; NM_001201790.2.
DR AlphaFoldDB; M9MSG8; -.
DR SMR; M9MSG8; -.
DR STRING; 7227.FBpp0289561; -.
DR PaxDb; M9MSG8; -.
DR PRIDE; M9MSG8; -.
DR EnsemblMetazoa; FBtr0335179; FBpp0307172; FBgn0264953.
DR GeneID; 34112; -.
DR KEGG; dme:Dmel_CG44122; -.
DR CTD; 34112; -.
DR FlyBase; FBgn0264953; Piezo.
DR VEuPathDB; VectorBase:FBgn0264953; -.
DR eggNOG; KOG1893; Eukaryota.
DR GeneTree; ENSGT00940000166986; -.
DR BioGRID-ORCS; 34112; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34112; -.
DR PRO; PR:M9MSG8; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0264953; Expressed in head epidermis primordium (Drosophila) and 49 other tissues.
DR ExpressionAtlas; M9MSG8; baseline and differential.
DR Genevisible; M9MSG8; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:FlyBase.
DR GO; GO:0005261; F:cation channel activity; IBA:GO_Central.
DR GO; GO:0008381; F:mechanosensitive ion channel activity; IDA:FlyBase.
DR GO; GO:0098655; P:cation transmembrane transport; IDA:FlyBase.
DR GO; GO:0071260; P:cellular response to mechanical stimulus; IMP:FlyBase.
DR GO; GO:0050982; P:detection of mechanical stimulus; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IMP:UniProtKB.
DR GO; GO:0007638; P:mechanosensory behavior; IMP:FlyBase.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR InterPro; IPR027272; Piezo.
DR InterPro; IPR031805; Piezo_dom.
DR InterPro; IPR031334; Piezo_RRas-bd_dom.
DR PANTHER; PTHR13167; PTHR13167; 1.
DR Pfam; PF15917; PIEZO; 1.
DR Pfam; PF12166; Piezo_RRas_bdg; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW Sensory transduction; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..2551
FT /note="Piezo-type mechanosensitive ion channel component"
FT /id="PRO_0000432404"
FT TRANSMEM 5..25
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TRANSMEM 106..126
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..226
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TRANSMEM 231..250
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TRANSMEM 320..340
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TRANSMEM 424..444
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TRANSMEM 516..536
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TRANSMEM 548..568
FT /note="Helical; Name=13"
FT /evidence="ECO:0000255"
FT TRANSMEM 588..608
FT /note="Helical; Name=14"
FT /evidence="ECO:0000255"
FT TRANSMEM 611..631
FT /note="Helical; Name=15"
FT /evidence="ECO:0000255"
FT TRANSMEM 639..659
FT /note="Helical; Name=16"
FT /evidence="ECO:0000255"
FT TRANSMEM 695..715
FT /note="Helical; Name=17"
FT /evidence="ECO:0000255"
FT TRANSMEM 819..839
FT /note="Helical; Name=18"
FT /evidence="ECO:0000255"
FT TRANSMEM 857..877
FT /note="Helical; Name=19"
FT /evidence="ECO:0000255"
FT TRANSMEM 910..930
FT /note="Helical; Name=20"
FT /evidence="ECO:0000255"
FT TRANSMEM 973..993
FT /note="Helical; Name=21"
FT /evidence="ECO:0000255"
FT TRANSMEM 994..1014
FT /note="Helical; Name=22"
FT /evidence="ECO:0000255"
FT TRANSMEM 1022..1042
FT /note="Helical; Name=23"
FT /evidence="ECO:0000255"
FT TRANSMEM 1071..1091
FT /note="Helical; Name=24"
FT /evidence="ECO:0000255"
FT TRANSMEM 1152..1172
FT /note="Helical; Name=25"
FT /evidence="ECO:0000255"
FT TRANSMEM 1174..1194
FT /note="Helical; Name=26"
FT /evidence="ECO:0000255"
FT TRANSMEM 1198..1218
FT /note="Helical; Name=27"
FT /evidence="ECO:0000255"
FT TRANSMEM 1239..1259
FT /note="Helical; Name=28"
FT /evidence="ECO:0000255"
FT TRANSMEM 1275..1295
FT /note="Helical; Name=29"
FT /evidence="ECO:0000255"
FT TRANSMEM 1718..1738
FT /note="Helical; Name=30"
FT /evidence="ECO:0000255"
FT TRANSMEM 1741..1761
FT /note="Helical; Name=31"
FT /evidence="ECO:0000255"
FT TRANSMEM 1770..1790
FT /note="Helical; Name=32"
FT /evidence="ECO:0000255"
FT TRANSMEM 1817..1837
FT /note="Helical; Name=33"
FT /evidence="ECO:0000255"
FT TRANSMEM 1937..1957
FT /note="Helical; Name=34"
FT /evidence="ECO:0000255"
FT TRANSMEM 1979..1999
FT /note="Helical; Name=35"
FT /evidence="ECO:0000255"
FT TRANSMEM 2008..2028
FT /note="Helical; Name=36"
FT /evidence="ECO:0000255"
FT TRANSMEM 2033..2053
FT /note="Helical; Name=37"
FT /evidence="ECO:0000255"
FT TRANSMEM 2075..2095
FT /note="Helical; Name=38"
FT /evidence="ECO:0000255"
FT TRANSMEM 2151..2171
FT /note="Helical; Name=39"
FT /evidence="ECO:0000255"
FT TRANSMEM 2431..2451
FT /note="Helical; Name=40"
FT /evidence="ECO:0000255"
FT REGION 354..375
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 731..772
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1426..1521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1592..1658
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1854..1876
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2522..2551
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..375
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1426..1445
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1449..1476
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1499..1521
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1594..1609
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1616..1635
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2522..2536
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2537..2551
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1640..1642
FT /note="Missing (in Ref. 1; AFB77909)"
FT /evidence="ECO:0000305"
FT CONFLICT 2326
FT /note="Y -> H (in Ref. 1; AFB77909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 2551 AA; 289447 MW; CC463BCC881AF6F0 CRC64;
MVFSYACMVL QRIVVPAVLV LAALMRPVGI SFVYLLMFFV SPFVPLATRR NFKGSVTAFF
IILLTLSTLV LLGHITLQIL AVSLTLPIYN CSFSERLLRH IGFVSFIDLQ PFAIIEWLVP
EVLVFATSLG SYLTVKRVAS QPVGAEQLEN GEVVDGQAEN AQTSSQPSAA DANGGDVQQA
TVTTPLQQQQ QQLRKRVSMI SQHIHFEGLV KISPLFCLAT LFFAAVLRPS VPGGFYFLIF
LLSGTYWATC QTLQRGFALL LRCVMVVLVL HSLSIVSYQT PWMQSHLNHT TLTARLIGLE
PLIESYCSPD IRVFLYNNKL SLDSYLNPFA LFFAYFALAL TTKHLIKPRL VRQSTRKART
PQPLESGSSV APSVTQRGND MQLESMEQRS EQENTTTSIL DQISYGFVSV GGFIYQNSYI
FTNILMMAWS IVYHSWLTFV LLLSANVLWM IPNQRKAMMR SSPFIVLYAE ALLIAQYIYG
MDLNNEELPT SVPTAGINLQ QIGFERPIEN QMRPCVPLIV KTAFVLMFWV TSRQFFKEKR
DRRRDSTLAD FIAPLQITVG SAGSSYLIND GKKTSKFLKK AGDVIKNLLV RLWIWLLVLV
IFLCAITGEN MTGFRICYMA LFLFFLLVFQ SSSKAWVKIM YGFWLFLIFY AMSILILIYT
YQFDKFDTYW SDYLNVSATL QKDIGLKRYQ TKDLFLHLVS PTIIVILTVI QVHYFHKRFI
ASLQQQPLAG GSAQQKPTET TALEPAPSKR RGSAGSLRKS QGPSAEAAPG ATTDFETSVR
DLVRISFRKI KNKSEYIFKN FKDVFWRFLE LHIMKAVYIA AFVCSVSEVC VLHIIFVGFC
VLGATSRKAV QVVISRLISF IVTVIVLSKM IYQIEYLSHS QHNVVCSDNR TANNAEWIGL
TKADKVTGGL MSLLRTYIIY MVIVTMHAVI SLRQLQMRVK IGALNAPPTK LLFPNIIRAD
AEKDLVGLVK YLLNFGFYKF GIEISLIALV STITYRQDIV AVVYALWLVV LLLLRRSQCA
KIWGVFQAFF AISILTQYIV LVGLPPSSCL VFPWDEGPFG EGIQRWAMLP GALHFNHVPK
LIFDFIVLVI LNRQKSIFCI EQRYASNDDY PGGSNRSVIA DIAQLGRVPF DNPTHDFCSY
IRNYSDILKN GVLCGFYWFT LAVVFLAGTN IADLLALGYL IGAFIFLWQG SDFYLRPIHT
IIFRWKWLLA FNVANILIKT SFQMAGCLFM TQLTKDCCWL VHMLGITCTS NVLTEQIMLP
EEAELALKPG ECPKITHQVV LLWDTICFAF IIFQLRIFKS HYFCHIITDT KANNILASRG
ADIIESLRHK QIAHRHDHEK QVLHKIKRKM ERIRATQQKM LRPLDKQTHF DEHGYPLPAP
TVRRRKEIKL HPHATRAGDY YMFEEMDDKF ELDLIHDEID FLEEENITES EMKMQRRKTL
YDKSKDAPTG EFPSTSKGIS KERDAATASS SASPAPTRDV GDLPVIPPPS TGLGREQTSK
ETSDSKSKME VDSGEVTAKD SDEDFDTNPI IRLLEGFLVT LTIRLNRFSR NYRFVNRILA
GEKKTLKESS SLNRLGLSSA AAMFHFLKSN LESDESEPPA SSSTPRRVVI APPNATEHSD
PTSTTLNTNT TTTPLSPPEP LQPLQPLQPN TTSTPQQQHQ HIRAAEEIIE LPVDTVDGVA
HRKQSINSSP PAKGAGEFNL EEENFAQRDH HIIVEVLISS WYALLANTDL ICYIVVFINQ
VVNASLISLP LPIMVFLWGT LSLPRPTKTF WVTLIAYTQA IVLIKCIFQF KLIWSNYHQL
PNQPLTPAKI FGVENKAHYA IYDLILLLVL FLHRYLLKSQ GLWKSGYKDT DNQFTKPTAS
IDERDDSDNL SQPDSRQLND DAAQKLSLQV SQASLPGSPE FSKTGINQLE RTKYTSSLYK
FFFSLVHKSR LATDVYALMF LCDFVNFFVL LFGFTAFGTQ QTESDEGVQT YLAENKVPIP
FLIMLLVQFL LIVIDRALYL RKALVNKIIF HFFSVIGIHI WMFFVVPAVT ERTFNSLAPP
IIFYVIKCFY MLLSSYQIKS GYPKRILGNF FTKGFSMVNM IAFKVYMQIP FLYELRTILD
WVCIDSTMTI FDWLKMEDIF SNIYLIRCTR QSETDFPAMR AQKKASLSKL IMGGTIVLLI
VICIWGPLCL FALGNAVGTS NVPFHVSLSI RIGPYDPIYT TNNYDSIFEI NPEMYSQMTN
AYIKEKQALT FIAGYDATDV AAVRLAGNSP SLWNIAPPDR QRLLNDLRNN HTLKARFSYS
LTRKAPAKGL KENVGDEHAI SLDESFEGRA ALIHMLSETH DVEPIYSNGT TNGTTPEVEE
VVVIPGMIPK FIKVLNSGDA AVVSVLSPKH YDYRPLVIKM HRDNETNGLW WEIRDYCNDT
FYNETLSKFA YSNCTSGIVM YTFNDKKFPS TFSFLTAGGI IGLYTTFVLL ASRFMKSFIG
GQNRKIMFED LPYVDRVLQL CLDIYLVREA LEFALEEDLF AKLLFLYRSP ETLIKWTRPK
EEYVDDDGDT DSIPSRMSVR RPEQLQPQQP Q
