PIE_DROME
ID PIE_DROME Reviewed; 582 AA.
AC Q9VKW2; Q9NGN6;
DT 25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Pineapple eye protein {ECO:0000303|PubMed:14704172, ECO:0000312|FlyBase:FBgn0005683};
GN Name=pie {ECO:0000312|FlyBase:FBgn0005683};
GN Synonyms=l(2)31Ek {ECO:0000312|FlyBase:FBgn0005683},
GN l(2)54 {ECO:0000312|FlyBase:FBgn0005683},
GN mat(2)ea-E {ECO:0000312|FlyBase:FBgn0005683},
GN mat(2)earlyQM47 {ECO:0000312|FlyBase:FBgn0005683},
GN mat(2)QM47 {ECO:0000312|FlyBase:FBgn0005683};
GN ORFNames=CG5354 {ECO:0000312|FlyBase:FBgn0005683};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:AAF63389.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF 391-GLN--SER-582.
RC TISSUE=Imaginal disk {ECO:0000312|EMBL:AAF63389.1};
RX PubMed=14704172; DOI=10.1093/genetics/165.4.1869;
RA Shi W., Stampas A., Zapata C., Baker N.E.;
RT "The pineapple eye gene is required for survival of Drosophila imaginal
RT disc cells.";
RL Genetics 165:1869-1879(2003).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|FlyBase:FBgn0005683}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|FlyBase:FBgn0005683};
RC TISSUE=Embryo {ECO:0000312|FlyBase:FBgn0005683};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=22413088; DOI=10.1534/g3.111.001651;
RA Xing Y., Kurtz I., Thuparani M., Legard J., Ruohola-Baker H.;
RT "Loss-of-Function Screen Reveals Novel Regulators Required for Drosophila
RT Germline Stem Cell Self-Renewal.";
RL G3 (Bethesda) 2:343-351(2012).
CC -!- FUNCTION: Required for survival of imaginal disk cells possibly by
CC regulation of cell apoptosis (PubMed:14704172). Required for germline
CC stem cell self-renewal through mediation of BMP signaling
CC (PubMed:22413088). {ECO:0000269|PubMed:14704172,
CC ECO:0000269|PubMed:22413088}.
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DR EMBL; AF247501; AAF63389.1; -; mRNA.
DR EMBL; AE014134; AAF52945.1; -; Genomic_DNA.
DR EMBL; AE014134; AGB92883.1; -; Genomic_DNA.
DR EMBL; AY061141; AAL28689.1; -; mRNA.
DR RefSeq; NP_001260348.1; NM_001273419.1.
DR RefSeq; NP_524740.2; NM_080001.4.
DR AlphaFoldDB; Q9VKW2; -.
DR SMR; Q9VKW2; -.
DR IntAct; Q9VKW2; 30.
DR STRING; 7227.FBpp0305674; -.
DR PaxDb; Q9VKW2; -.
DR DNASU; 44315; -.
DR EnsemblMetazoa; FBtr0080046; FBpp0079636; FBgn0005683.
DR EnsemblMetazoa; FBtr0333490; FBpp0305674; FBgn0005683.
DR GeneID; 44315; -.
DR KEGG; dme:Dmel_CG5354; -.
DR UCSC; CG5354-RA; d. melanogaster.
DR CTD; 44315; -.
DR FlyBase; FBgn0005683; pie.
DR VEuPathDB; VectorBase:FBgn0005683; -.
DR eggNOG; KOG1084; Eukaryota.
DR GeneTree; ENSGT00950000182865; -.
DR HOGENOM; CLU_558100_0_0_1; -.
DR InParanoid; Q9VKW2; -.
DR OMA; EPFFYLV; -.
DR OrthoDB; 199998at2759; -.
DR PhylomeDB; Q9VKW2; -.
DR BioGRID-ORCS; 44315; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 44315; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0005683; Expressed in adult abdomen and 21 other tissues.
DR ExpressionAtlas; Q9VKW2; baseline and differential.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0042078; P:germ-line stem cell division; IMP:FlyBase.
DR GO; GO:0036335; P:intestinal stem cell homeostasis; IMP:FlyBase.
DR GO; GO:2000035; P:regulation of stem cell division; IMP:UniProtKB.
DR GO; GO:0035019; P:somatic stem cell population maintenance; IMP:FlyBase.
DR Gene3D; 3.30.40.10; -; 2.
DR InterPro; IPR034732; EPHD.
DR InterPro; IPR011011; Znf_FYVE_PHD.
DR InterPro; IPR001965; Znf_PHD.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR SMART; SM00249; PHD; 2.
DR SUPFAM; SSF57903; SSF57903; 1.
DR PROSITE; PS51805; EPHD; 1.
PE 1: Evidence at protein level;
KW Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT CHAIN 1..582
FT /note="Pineapple eye protein"
FT /id="PRO_0000455703"
FT ZN_FING 6..44
FT /note="C2HC pre-PHD-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01146"
FT ZN_FING 72..119
FT /note="PHD-type; atypical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01146"
FT REGION 6..119
FT /note="Extended PHD domain (ePHD)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01146"
FT REGION 292..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 323..422
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 341..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 380..422
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 391..582
FT /note="Missing: Increases apoptosis in the eye imaginal
FT disk thereby leading to a rough eye phenotype."
FT /evidence="ECO:0000269|PubMed:14704172"
FT CONFLICT 131
FT /note="N -> K (in Ref. 1; AAF63389)"
FT /evidence="ECO:0000305"
FT CONFLICT 398
FT /note="T -> I (in Ref. 1; AAF63389)"
FT /evidence="ECO:0000305"
FT CONFLICT 457
FT /note="S -> P (in Ref. 1; AAF63389)"
FT /evidence="ECO:0000305"
FT CONFLICT 467
FT /note="C -> S (in Ref. 1; AAF63389)"
FT /evidence="ECO:0000305"
FT CONFLICT 477
FT /note="K -> E (in Ref. 1; AAF63389)"
FT /evidence="ECO:0000305"
FT CONFLICT 550
FT /note="N -> K (in Ref. 1; AAF63389)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 582 AA; 66071 MW; 0814817298AEF7A2 CRC64;
MEDNKELQCL ICKYSDTDDL VFGEWMIVRN LQVHYFCLLL STHLPQRGGD SSGILGFLLR
DIREEAAAAE KRKCWYCNKI GASLQCDRCR SLFHLKCGLE NRAVFEFCGQ YKSYCYKCRP
MDDYKRQLQS NPPRNATCPI CFSSIYKVEL HCVVYGDCCR LGFAHKKCMR QYALTSGYYL
RCIWCRSERF RDSIRLQSVF VPDRDATWEK QRNAYRELHE RNLKCDQPNC LCPSGRTYNR
LSWVILCCSS CAATSAHLKC LVGALRLPKK RERTDFKCSM CLDVERRIAE GPARTTEETN
ADGDNQVDGS FYVQKLGPDA ATRSLTQTPV FSEEDESERS SNITVIFSQP KSNATSERLS
LSPPQEEMIV EIPDSPEASP KTSIDENHSP QPIARRDTSD SPQPIAASEI PDSPQPTAAS
EIPDLPQTTA INVNPELTQQ TALNTIPHSP QPEASFSTQL VSQTFDCPQP QEQAVAKAPN
SPSLPKEDPN TLLVLKSGFQ CPGEPFFYLV IYEFEHGTCM GECIGTCVLR FKEDDPRIQD
TSQAALERVN ITPDDVWCRS EDRGIFEHIE KFHEWYRSEG FS
