ID   PIF1_DANRE              Reviewed;         639 AA.
AC   Q7ZV90;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=ATP-dependent DNA helicase PIF1 {ECO:0000255|HAMAP-Rule:MF_03176};
DE            EC=3.6.4.12 {ECO:0000255|HAMAP-Rule:MF_03176};
DE   AltName: Full=DNA repair and recombination helicase PIF1 {ECO:0000255|HAMAP-Rule:MF_03176};
GN   Name=pif1; ORFNames=zgc:56161;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the
CC       maintenance of both mitochondrial and nuclear genome stability.
CC       {ECO:0000255|HAMAP-Rule:MF_03176}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_03176};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_03176};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_03176}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|HAMAP-Rule:MF_03176}.
CC       Mitochondrion {ECO:0000255|HAMAP-Rule:MF_03176}.
CC   -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC       {ECO:0000255|HAMAP-Rule:MF_03176}.
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DR   EMBL; BC045956; AAH45956.1; -; mRNA.
DR   RefSeq; NP_942102.1; NM_198807.1.
DR   AlphaFoldDB; Q7ZV90; -.
DR   SMR; Q7ZV90; -.
DR   STRING; 7955.ENSDARP00000022087; -.
DR   PaxDb; Q7ZV90; -.
DR   GeneID; 326729; -.
DR   KEGG; dre:326729; -.
DR   CTD; 80119; -.
DR   ZFIN; ZDB-GENE-030131-4928; pif1.
DR   eggNOG; KOG0987; Eukaryota.
DR   InParanoid; Q7ZV90; -.
DR   OrthoDB; 931726at2759; -.
DR   PhylomeDB; Q7ZV90; -.
DR   PRO; PR:Q7ZV90; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR   GO; GO:0043139; F:5'-3' DNA helicase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR   GO; GO:0000002; P:mitochondrial genome maintenance; IEA:UniProtKB-UniRule.
DR   GO; GO:0000723; P:telomere maintenance; IEA:InterPro.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_03176; PIF1; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR010285; DNA_helicase_pif1-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF05970; PIF1; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   2: Evidence at transcript level;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Mitochondrion; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..639
FT                   /note="ATP-dependent DNA helicase PIF1"
FT                   /id="PRO_0000295093"
FT   DNA_BIND        577..596
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03176"
FT   BINDING         228..235
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03176"
SQ   SEQUENCE   639 AA;  71411 MW;  E318B2C645F354CD CRC64;
     MLGQMEPAEL QCTVAVERLN PAGQAIKRQL IRKGTVTLGR NEFQEMILRV HDGKAPQNFM
     LRDFQLFTRF AKDGKCTVKF VPENTQVLVS DCPPDRLKMF LKTLSIKHQA SQSSKPLSDR
     EKLRAGLPRA FETVSPLQLK DVEKANELRS RVNAPMQPRC LAERPVNKVL GDRKQVKRPR
     PDCDASPVKA LHPNKKPVLV LPVAQKLSKE QTAVLNAVLS GKNVFFTGSA GTGKSFLLKR
     IVGSLPPKST YATASTGVAA CHIGGTTLHS FAGIGSGSAP LEQCIELAQR PGVLRHWTSC
     KHLIIDEISM VEAEFFDKLE AIARSIRRST EPFGGIQLIV CGDFLQLPPV TKGKEKANFC
     FQSRSWRKCI HMNMELMEVR RQTDKTFISL LQAVRVGRVT EEVTAQLLKS ANHCIERDGI
     LATRLCTHKD DVELTNENKL KQLPGVVRMY EAVDSDPMLV QTIDAQSPVS RLLQLKVGAQ
     VMLTKNLDVQ RGLVNGARGV VVDFQPGNQG LPRVRFLCGA VEVMKRERWM FKAPGGLYLS
     RQQLPLKLAW AISIHKSQGM TLDCVEISLA RVFESGQAYV ALSRARSLEG LRVMDFDPRV
     VQANQDVLIF YKRLRKERLL MQSSMNDFVG QSNKENSRW