ID   PIF3_TRYB2              Reviewed;         812 AA.
AC   Q383A1;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=ATP-dependent DNA helicase PIF3;
DE            EC=3.6.4.12;
DE   AltName: Full=DNA repair and recombination helicase PIF3;
GN   Name=PIF3; ORFNames=Tb11.01.3660;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA   Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA   Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA   Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA   Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA   Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA   Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA   Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA   Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA   Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA   Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA   Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA   Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA   Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA   Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA   Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19646907; DOI=10.1016/j.molcel.2009.07.004;
RA   Liu B., Wang J., Yaffe N., Lindsay M.E., Zhao Z., Zick A., Shlomai J.,
RA   Englund P.T.;
RT   "Trypanosomes have six mitochondrial DNA helicases with one controlling
RT   kinetoplast maxicircle replication.";
RL   Mol. Cell 35:490-501(2009).
CC   -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the
CC       maintenance of genome stability. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19646907}.
CC   -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CH464491; EAN80130.1; -; Genomic_DNA.
DR   RefSeq; XP_829242.1; XM_824149.1.
DR   AlphaFoldDB; Q383A1; -.
DR   STRING; 5691.EAN80130; -.
DR   PaxDb; Q383A1; -.
DR   GeneID; 3665724; -.
DR   KEGG; tbr:Tb11.01.3660; -.
DR   eggNOG; KOG0987; Eukaryota.
DR   InParanoid; Q383A1; -.
DR   Proteomes; UP000008524; Chromosome 11 Scaffold 1.
DR   GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR   GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IDA:GeneDB.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IDA:GeneDB.
DR   GO; GO:0051276; P:chromosome organization; ISA:GeneDB.
DR   GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; ISA:GeneDB.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR   GO; GO:0000723; P:telomere maintenance; ISA:GeneDB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR010285; DNA_helicase_pif1-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF05970; PIF1; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; DNA damage; DNA recombination; DNA repair;
KW   DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..812
FT                   /note="ATP-dependent DNA helicase PIF3"
FT                   /id="PRO_0000423749"
FT   DNA_BIND        741..761
FT                   /evidence="ECO:0000255"
FT   BINDING         247..254
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   812 AA;  90236 MW;  0333512DAFC34311 CRC64;
     MRLAAESSLV FFVSHFYFER FFSFVMVAYT LDTLFASDID SATLFYSEGC GPIRLVALSA
     QMRRLVRLGP IRVAKACVEV RPMAPTKEFF EEGRQLQHIE AAGVGDFPDS ERSGDTFVNP
     FTGRLTPTWG RVAKELFSLG FEHSIVNPFR LVWNPTKAAL VSDAALQPFR TSTVTWRRNL
     AALLSRRDAA DAVGEEIHRL YNEITSAYLP PKLDTMHDPR LSTMTMTPDQ QNVIRCALRG
     YSMFIGGSAG TGKTVLLKAI HRKLTEMGLR VAMTATTGVA SVQLGGCTFH LAFGVPIKGE
     EGTRKRWDSN AFRAVDVVII DEVSLLDAEL FETFEEEARM ARLQQSPFGG LQVIACGDFL
     QLAMMDVSIG GPCYQSHAFR HLIPVCLVTS MRQAQGDPFC ELLGQLRVGK FDKKAFKALD
     RPVSGDANNV TYIFPRRCDA QRLNDEKLCE LRSEEMIFAP QRGPLQLVGN FTPAGLVDWG
     RKKDFPKREK IITVLSEEIK RITGVDIVDH NIVVMPAGGE KNAVLIRLRH SEDRNVLICK
     NGGSKEHGAS NEGGAEESHW RAILEATAGR LKGKLHQIYN QDPHNFIPPS VSLMLADASL
     HPNAELISPL RLKLGCRVMI NRNLSRTVSN GSVGIVEAFA APNLDLFPRR HETSPKAFHT
     WSLERNGFQR LPIVRLLSGE VVQLPPLSVM IGGTPSTYFY GHELFVLPLQ LGYGFTVHKV
     QGLTLEGTVV LDCKKFFECP HLVYVACSRV RSMDQLIVRN VRSDMIIVRQ SALDFTNALR
     DASVMSSLDP PDGCTRASWV RRLSPLLVGL TD