ID   PIF4_TRYB2              Reviewed;         819 AA.
AC   Q381V6;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=ATP-dependent DNA helicase PIF4;
DE            EC=3.6.4.12;
DE   AltName: Full=DNA repair and recombination helicase PIF4;
DE   Flags: Precursor;
GN   Name=PIF4; ORFNames=Tb11.01.6420;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA   Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA   Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA   Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA   Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA   Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA   Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA   Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA   Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA   Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA   Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA   Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA   Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA   Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA   Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA   Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19646907; DOI=10.1016/j.molcel.2009.07.004;
RA   Liu B., Wang J., Yaffe N., Lindsay M.E., Zhao Z., Zick A., Shlomai J.,
RA   Englund P.T.;
RT   "Trypanosomes have six mitochondrial DNA helicases with one controlling
RT   kinetoplast maxicircle replication.";
RL   Mol. Cell 35:490-501(2009).
CC   -!- FUNCTION: DNA-dependent ATPase and 5'-3' DNA helicase required for the
CC       maintenance of mitochondrial (kinetoplast) genome stability.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix, kinetoplast
CC       {ECO:0000269|PubMed:19646907}. Note=Localizes predominantly to the kDNA
CC       disk.
CC   -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; CH464491; EAN80425.1; -; Genomic_DNA.
DR   RefSeq; XP_829537.1; XM_824444.1.
DR   AlphaFoldDB; Q381V6; -.
DR   STRING; 5691.EAN80425; -.
DR   PaxDb; Q381V6; -.
DR   GeneID; 3665357; -.
DR   KEGG; tbr:Tb11.01.6420; -.
DR   VEuPathDB; TriTrypDB:Tb927.11.14790; -.
DR   eggNOG; KOG0987; Eukaryota.
DR   InParanoid; Q381V6; -.
DR   Proteomes; UP000008524; Chromosome 11 Scaffold 1.
DR   GO; GO:0005737; C:cytoplasm; IDA:GeneDB.
DR   GO; GO:0020023; C:kinetoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IDA:GeneDB.
DR   GO; GO:0005657; C:replication fork; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IC:GeneDB.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003678; F:DNA helicase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000287; F:magnesium ion binding; IC:GeneDB.
DR   GO; GO:0051276; P:chromosome organization; ISA:GeneDB.
DR   GO; GO:0032508; P:DNA duplex unwinding; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; ISA:GeneDB.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IBA:GO_Central.
DR   GO; GO:0000723; P:telomere maintenance; ISA:GeneDB.
DR   Gene3D; 3.40.50.300; -; 2.
DR   InterPro; IPR010285; DNA_helicase_pif1-like.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF05970; PIF1; 1.
DR   SUPFAM; SSF52540; SSF52540; 2.
PE   3: Inferred from homology;
KW   ATP-binding; DNA damage; DNA recombination; DNA repair; DNA-binding;
KW   Helicase; Hydrolase; Kinetoplast; Mitochondrion; Nucleotide-binding;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..84
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           85..819
FT                   /note="ATP-dependent DNA helicase PIF4"
FT                   /id="PRO_0000423750"
FT   DNA_BIND        734..754
FT                   /evidence="ECO:0000255"
FT   REGION          76..101
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         126..133
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   819 AA;  89458 MW;  2CB7F06146244759 CRC64;
     MLLNSTRTLL LTYGRLLFAP GREGRQSRLA KGVSAWTVAA EGEKGGQLSP APESTSCCGA
     AEVNEIKERD TKTCRQASSA GHNDLGLQEK EKSSGDESAF SSLGLNEEKR RALTLVLDGA
     PLFIGGGAGV GKSYMIQSIV TALRAKDLDV VVTASTGIAA LNIGGSTFHS TFGVRVTSVG
     NSETNESCAV SILRYSKSLL AKVDVIVVDE VSLLHARHLE GLDIAARGAP GRIPHLPFGG
     IQVILCGDFM QLMHSTENCT SQDGGGDAGN KIGYRGDETT ENTAGQNRSD VSSTAAVTDQ
     GHIMSAVKNI CVGERQRTSS GLIFESPLFL TCLLHLQLCE VKRHGDTAFL NDLNKLRQGV
     LTRRMMRSAL VNPEDPNAIQ LYPTRRSVAA FNESKMLELD GEEHLFRSIV ESAGLSGPKG
     HASPNSRGAN NVDGCNDVVV LHFLEKMRSS RRWQREVKKF VGQICTRCGI SGIATSVVAP
     PYSSRQPYLK VYVHFCVSKQ YDCLYPVAKM KAEWERSYYG TTPESKSARR FFGRVLFEVK
     HKNSLSTFLR ASLKQAYSKV IESDNVLQSK RLKVGCRVIL LRNLSNEYVN GSTGTVIGFQ
     PVNKSRHLFP KGIRTQLSRK VYASLSRRPV VSSDSSAGSS ENSYGGNGKE QALDVVNYDD
     VIVPIVRMDA DGKDVAIPWL SLPLPDLQDR VFCTARVVTM PLVPAYAFTV HKTQGLTLDH
     SILLDCKGFF PCNHIIYVAA SRVKKFSQLR MINVSPRMVT VHPGALHFSS SLPNVAEAET
     KWKKWKELQR MVNNGKALSA SNPSVLELAL YCATWKHHK