ID   PIF8_TRYB2              Reviewed;         693 AA.
AC   Q57VU6; D6XK18;
DT   16-OCT-2013, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=ATP-dependent DNA helicase-like PIF8;
DE   AltName: Full=DNA repair and recombination protein PIF8;
DE   Flags: Precursor;
GN   Name=PIF8; ORFNames=Tb927.7.1000;
OS   Trypanosoma brucei brucei (strain 927/4 GUTat10.1).
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma.
OX   NCBI_TaxID=185431;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=927/4 GUTat10.1 {ECO:0000312|Proteomes:UP000008524};
RX   PubMed=16020726; DOI=10.1126/science.1112642;
RA   Berriman M., Ghedin E., Hertz-Fowler C., Blandin G., Renauld H.,
RA   Bartholomeu D.C., Lennard N.J., Caler E., Hamlin N.E., Haas B., Bohme U.,
RA   Hannick L., Aslett M.A., Shallom J., Marcello L., Hou L., Wickstead B.,
RA   Alsmark U.C.M., Arrowsmith C., Atkin R.J., Barron A.J., Bringaud F.,
RA   Brooks K., Carrington M., Cherevach I., Chillingworth T.J., Churcher C.,
RA   Clark L.N., Corton C.H., Cronin A., Davies R.M., Doggett J., Djikeng A.,
RA   Feldblyum T., Field M.C., Fraser A., Goodhead I., Hance Z., Harper D.,
RA   Harris B.R., Hauser H., Hostetler J., Ivens A., Jagels K., Johnson D.,
RA   Johnson J., Jones K., Kerhornou A.X., Koo H., Larke N., Landfear S.,
RA   Larkin C., Leech V., Line A., Lord A., Macleod A., Mooney P.J., Moule S.,
RA   Martin D.M., Morgan G.W., Mungall K., Norbertczak H., Ormond D., Pai G.,
RA   Peacock C.S., Peterson J., Quail M.A., Rabbinowitsch E., Rajandream M.A.,
RA   Reitter C., Salzberg S.L., Sanders M., Schobel S., Sharp S., Simmonds M.,
RA   Simpson A.J., Tallon L., Turner C.M., Tait A., Tivey A.R., Van Aken S.,
RA   Walker D., Wanless D., Wang S., White B., White O., Whitehead S.,
RA   Woodward J., Wortman J., Adams M.D., Embley T.M., Gull K., Ullu E.,
RA   Barry J.D., Fairlamb A.H., Opperdoes F., Barrell B.G., Donelson J.E.,
RA   Hall N., Fraser C.M., Melville S.E., El-Sayed N.M.A.;
RT   "The genome of the African trypanosome Trypanosoma brucei.";
RL   Science 309:416-422(2005).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=19646907; DOI=10.1016/j.molcel.2009.07.004;
RA   Liu B., Wang J., Yaffe N., Lindsay M.E., Zhao Z., Zick A., Shlomai J.,
RA   Englund P.T.;
RT   "Trypanosomes have six mitochondrial DNA helicases with one controlling
RT   kinetoplast maxicircle replication.";
RL   Mol. Cell 35:490-501(2009).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=22220754; DOI=10.1111/j.1365-2958.2011.07938.x;
RA   Wang J., Englund P.T., Jensen R.E.;
RT   "TbPIF8, a Trypanosoma brucei protein related to the yeast Pif1 helicase,
RT   is essential for cell viability and mitochondrial genome maintenance.";
RL   Mol. Microbiol. 83:471-485(2012).
CC   -!- FUNCTION: Required for the maintenance of mitochondrial (kinetoplast)
CC       genome stability. Essential for cell viability. Has probably no
CC       helicase activity, missing at least two of seven conserved helicase
CC       motifs. {ECO:0000269|PubMed:22220754}.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix, kinetoplast
CC       {ECO:0000269|PubMed:19646907, ECO:0000269|PubMed:22220754}.
CC       Note=Localizes primarily to the distal face of the kDNA disk.
CC       Localization varies with different kDNA replication stages.
CC   -!- SIMILARITY: Belongs to the helicase family. PIF1 subfamily.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC159437; AAX70273.1; -; Genomic_DNA.
DR   EMBL; CP000070; AAZ12165.1; -; Genomic_DNA.
DR   RefSeq; XP_845724.1; XM_840631.1.
DR   AlphaFoldDB; Q57VU6; -.
DR   STRING; 5691.AAZ12165; -.
DR   PaxDb; Q57VU6; -.
DR   GeneID; 3658305; -.
DR   KEGG; tbr:Tb927.7.1000; -.
DR   VEuPathDB; TriTrypDB:Tb927.7.1000; -.
DR   eggNOG; KOG0987; Eukaryota.
DR   InParanoid; Q57VU6; -.
DR   OMA; MSIAQWT; -.
DR   Proteomes; UP000008524; Chromosome 7.
DR   GO; GO:0020023; C:kinetoplast; IDA:GeneDB.
DR   GO; GO:0005739; C:mitochondrion; IDA:GeneDB.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0000002; P:mitochondrial genome maintenance; IMP:GeneDB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA recombination; DNA repair; Kinetoplast; Mitochondrion;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..67
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           68..693
FT                   /note="ATP-dependent DNA helicase-like PIF8"
FT                   /id="PRO_0000423754"
FT   REGION          300..348
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        318..332
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   693 AA;  77920 MW;  D9755C6350543F14 CRC64;
     MTRCRLVGAS SGGKYISTTN AKTRPVMGCL RFASRLSALG STQQPKFFAL PLLATTRGFG
     HLVREVDMRG FRGDFGQLRH KNLASTQRYV TDALGAKKNV IFCGAYHTGK LTILKAIGEA
     CEARGKKVAY VSCNPQRASR FGGFLLYHFV GLRYLFRNEI PSRDQLDGAL ERHARLCEST
     YAGCVPSLKS VDVLIFDAVD QLEPTILASM DAVCRRLRGK PNDAFGGLRV FAAADFWRLP
     VHPSSDTGGY IFQLDNWGEL FPKQHLLKKI YGQTKALTTL VNKAYYGQLS AEDIEELEAR
     SSAGRTESGA ACGGSKKEAS ETSGSFSVSD DGSRQVEVEE EGAGSGLLPP QRLISNAEAI
     IKFTSRFPKQ PSIRVLPPRF RTLKRTEIGN YIVNMMVQSS TLHSFGLVDS LNLDIGASVH
     LLFDGTADFG VTAGTVGEVL QVKEHALVVH FPSVHRTVDV PRMRISVYHP FYPEVRYEVQ
     QFPLYPRHCI SPINMLTYHH AYQVHIDCHQ LADTNDLGNI LARMRTFDDF TIERVHDFAH
     LDGMVHEPTR IYYQRIDNQP LSSAAEQWCR NCKSYVSTSD FYTHWDKCVR QVRWCTVCNT
     RIPLERLGPH QEKHQIVLCL DCGRAVEWRR WEGHRLTCSA MMREVSPENE FLPLRTRQLA
     LEMGLDKRDL HTMKGFNRGM LPKSRKQCNG GEA