ID   PIFA_ECOLI              Reviewed;         741 AA.
AC   P96329;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Phage T7 exclusion protein;
GN   Name=pifA; OrderedLocusNames=ECOK12F040;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 411-735.
RC   STRAIN=K12;
RA   Molineux I.J.;
RL   Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   FUNCTION.
RX   PubMed=5283385; DOI=10.1038/newbio231037a0;
RA   Morrison T.G., Malamy M.H.;
RT   "T7 translational control mechanisms and their inhibition by F factors.";
RL   Nature New Biol. 231:37-41(1971).
CC   -!- FUNCTION: Responsible for the exclusion of phage T7 by plasmid F.
CC       Growth of bacteriophage T7 is inhibited in cells of E.coli that carries
CC       the plasmid F. {ECO:0000269|PubMed:5283385}.
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DR   EMBL; AP001918; BAA97910.1; -; Genomic_DNA.
DR   EMBL; U89941; AAB48033.1; -; Genomic_DNA.
DR   RefSeq; NP_061419.1; NC_002483.1.
DR   RefSeq; WP_000698737.1; NZ_CP014273.1.
DR   AlphaFoldDB; P96329; -.
DR   PRIDE; P96329; -.
DR   PRO; PR:P96329; -.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR011646; KAP_P-loop.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF07693; KAP_NTPase; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   4: Predicted;
KW   Plasmid.
FT   CHAIN           1..741
FT                   /note="Phage T7 exclusion protein"
FT                   /id="PRO_0000068413"
FT   DOMAIN          27..334
FT                   /note="KAP NTPase"
FT   CONFLICT        701..741
FT                   /note="DQVSPFFDDWPAVEEKLKVMLSGNELTPEQEALKTALENDD -> VTRSVRS
FT                   LMTGQQSKRS (in Ref. 2; AAB48033)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   741 AA;  84966 MW;  4C373DF929AFCD65 CRC64;
     MKILRQLWNQ KGLDAAVEDV PEDRYGFGNI AENISRSILT LPLEASNVVG IEGAWGSGKT
     SLLNLILRNL ALKKDAHTHV LHISPWLSGG SPVEALFLPV ATVIQQEMEI RYPPKGFKKL
     WRKYLLSPEA QKVIEYAQDT SSRVLPLVQY IGQFSSIINW IAGGIKVFSD SRLAVDQKTT
     TKLRAEIAGQ LVSLDLKFIV VMDDLDRLEP SQVAEVFRLV RAVADLPRFT HILCYDRQII
     THAVEHALNI EDGSRYLQKI IQLSFKLPRP EAFDLRNEFR QRAEALYQQI NNQPPDSGMV
     RDLIAVTDTY GAALSTPREI HQAINSLIFL YPGMRDFVYF PDLCLLQLIR VTNPALYDWT
     EHYLTERSVI ETGQGMLSDG EKADFREGLI RCMKTFRASN ADSFLTLADW IPGISGHNDE
     YLNLFEPVSE DFRHIQTTGK RLSSLTHWRY YFAFSSPQNV LPPEFFRQLF EQAGVSEKQQ
     QLSELLLSKI NSVGSLSGTW FEHILSRLTP GLIRERNFEE CAGLVHFFFD HTDEVSTRFS
     IRNPWFSLRE MAINEVVRHL LKHMQDIDET RTITLMEKLI VTGASPFWIA DFMRDLIWEH
     GLAQNAVPSP SDALFSRDIT ERLRDRFAER MNQPELQQQL LLRKSLLGYL YAWRDMSSGE
     TVKQWVREVT TTDEGLVNLL IRLQTSVFSS HRGAYRRIAR DQVSPFFDDW PAVEEKLKVM
     LSGNELTPEQ EALKTALEND D