ID   PIFC_ECOLI              Reviewed;         362 AA.
AC   P10030; P13950;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Transcriptional repressor PifC;
DE            Short=Protein C;
GN   Name=pifC; Synonyms=C, repC; OrderedLocusNames=ECOK12F041;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3027660; DOI=10.1093/nar/14.24.9699;
RA   Caughey P.A., de Feyter R., Lane H.E.D.;
RT   "The miniF plasmid C protein: sequence, purification and DNA binding.";
RL   Nucleic Acids Res. 14:9699-9712(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Eichenlaub R.;
RT   "F plasmid DNA complete mini-F region (F coordinates 40.301F to 49.869F).";
RL   Submitted (AUG-1986) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-172.
RX   PubMed=6096563; DOI=10.1016/s0022-2836(84)80004-2;
RA   Lane D., Hill D., Caughey P., Gunn P.;
RT   "The mini-F primary origin. Sequence analysis and multiple activities.";
RL   J. Mol. Biol. 180:267-282(1984).
RN   [5]
RP   CHARACTERIZATION.
RX   PubMed=6413493; DOI=10.1128/jb.156.1.338-347.1983;
RA   Miller J.F., Malamy M.H.;
RT   "Identification of the pifC gene and its role in negative control of F
RT   factor pif gene expression.";
RL   J. Bacteriol. 156:338-347(1983).
CC   -!- FUNCTION: Transcription repression of its own gene by binding to the
CC       PIF operator (pifO) and replication initiation from the primary origin
CC       (ori-1). Transcriptional repressor of the pifA and pifB.
CC   -!- MISCELLANEOUS: C protein has a much higher affinity for pifO than for
CC       ori-1.
CC   -!- MISCELLANEOUS: C protein, like p22 proteins, contains a number of
CC       alpha-helical regions and these may be involved in forming a pattern of
CC       non-covalent contacts with DNA which is different from that formed by
CC       most DNA-binding proteins.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAA24897.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAA97911.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=CAA25877.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; M12987; AAA24897.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X04968; CAA28641.1; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97911.1; ALT_INIT; Genomic_DNA.
DR   EMBL; X01740; CAA25877.1; ALT_INIT; Genomic_DNA.
DR   PIR; A24710; A24710.
DR   RefSeq; NP_061420.1; NC_002483.1.
DR   RefSeq; WP_000952217.1; NZ_CP014273.1.
DR   AlphaFoldDB; P10030; -.
DR   PRIDE; P10030; -.
DR   PRO; PR:P10030; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   DNA-binding; Plasmid; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..362
FT                   /note="Transcriptional repressor PifC"
FT                   /id="PRO_0000068414"
FT   CONFLICT        316..362
FT                   /note="GFFGIDGLRIFMPAEAFETLVRELATRCQEGPLAEALTGLRCLYGDL -> A
FT                   SSG (in Ref. 2; AAA24897)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   362 AA;  40653 MW;  C15F82156CE300C8 CRC64;
     MLSQLNLRFH KKLIEALKTR AGRENTSVNA LAERFLDDGL KTVAPGDGYF QLIADPEATV
     RQLYRHIILG QTFGTSALSR DELRFVLVHV REAFLRGHNR LATLPALDTL LDITGNLLAW
     QVEHDRPVDG HYLKGIFRLA GKNWTEEFEA FRAALRPVVD QMYAEHLLRP LESDCFGLAE
     VPDAVLAEIF TLPRLKAVFP LMLRGLDWNT EQARTLAQEL RPVISAVTET IEAGTLRLEI
     RVDGQHPGER PGAWYTTPRL HLLITGQDFV VPYGWEALSE LLGLFTLYAR HPEALTHGHQ
     GERVMFSPPG NVTPEGFFGI DGLRIFMPAE AFETLVRELA TRCQEGPLAE ALTGLRCLYG
     DL